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目的:通过建立大鼠双侧卵巢切除及异丙肾上腺素诱导的心肌损伤模型,研究雌激素对异丙肾上腺素致大鼠心肌损伤及心肌细胞凋亡的影响。方法50只雌性 SD大鼠行双侧卵巢切除术和假手术后分为5组(每组10只):假手术组(Sham组);双侧卵巢切除组(OVX组);心肌损伤组(OVX+ISO+Vehi组),雌激素4μg· kg-1· d-1治疗组(OVX+ISO+E2 a组),雌激素40μg·kg-1·d-1治疗组(OVX+ISO+E2 b组)。分别测量大鼠大体指标,颈总动脉置管监测心脏血流动力学参数,分离培养单个心肌细胞观察形态与收缩功能改变,以及蛋白免疫印迹法检测心肌细胞凋亡蛋白的表达。结果异丙肾上腺素明显降低心肌功能,增加心肌细胞肥大与凋亡,降低单个心肌细胞收缩功能(P<0.05)。高剂量雌激素(40μg·kg-1·d-1)替代治疗显著改善异丙肾上腺素引起的心肌损伤与心肌功能下降(P<0.05),并通过增加 Bcl-2蛋白表达,减少 Bax蛋白表达与 Caspase-3的激活,降低心肌细胞肥大与凋亡(P<0.05)。而低剂量雌激素(4μg·kg-1·d-1)只表现出轻度抗异丙肾上腺素心肌损伤的作用,但差异无统计学意义(P>0.05)。结论适宜剂量的雌激素替代治疗通过降低心肌细胞凋亡,提高心肌细胞收缩功能,从而对异丙肾上腺素诱导的心肌损伤发挥保护作用。 相似文献
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Yizhu Dong Jianchun Huang Xing Lin Shijun ZhangYang Jiao Tao LiangZhaoni Chen Renbin Huang 《Journal of ethnopharmacology》2014
Ethnopharmacological relevance
Yulangsan polysaccharide (YLSPS) is often used in popular folk medicine in the Guangxi Zhuang Autonomous Region of China as a chief ingredient of Millettia pulchra, which is used as an hepatic protection, anti-aging and memory improving agent. In this study, the hepatoprotective effects of YLSPS against isoniazid (INH) or rifampicin and isoniazid (RFP+INH)-induced liver injury were investigated in mice.Materials and methods
The liver injury was induced by intragastric administration of INH or RFP+INH daily for 10 days. During the experiment, the model group received INH or RFP+INH only, and the normal control group received an equal volume of saline. Treatment groups received not only INH or RFP+INH but also the corresponding drugs, DDB (200 mg/kg/day) or YLSPS (100, 200, and 400 mg/kg/day) 2 h after the administration of INH or RFP+INH.Results
Analysis experiments showed that YLSPS significantly alleviated liver injury as indicated by the decreased levels of ALT and AST and the increased levels of SOD, GSH and GSH-Px. Moreover, YLSPS could effectively reduce the pathological tissue damage. The research on the mechanisms underlying the hepatoprotective effect showed that YLSPS was able to reduce lipid peroxidation and activate the anti-oxidative defense system.Conclusion
Our results show that YLSPS is effective in attenuating hepatic injury in the INH or RFP+INH-induced mouse model, and could be developed as a new drug for treatment of liver injury. 相似文献3.
目的 研究低压、控压膀胱冲洗在经尿道前列腺电切(TUR—P)中的价值。方法 将我院2003年1月至2005年5月需要行TUR—P患者98例随机分3组。常规冲洗电切组32例;低压、控压冲洗组34例;低压非控压组32例。常规组冲洗压80cmH2O、冲放式冲洗。低压、控压冲洗组经耻骨上膀胱穿刺造瘘,30-35cmH2O压力持续冲洗,利用引流管虹吸作用加快液体流速,切除突入膀胱的腺体时让膀胱保持适度充盈。低压非控压组经耻骨上膀胱穿刺造瘘,30—35cmH2O压力持续冲洗。结果 常规电切组平均手术时间(71±12)min;出血量(110±21)ml,电切综合征发生3例;膀胱或输尿管损伤5例。低压、控压持续冲洗组手术时间(44±11)min,出血量(49±15)ml,无电切综合征发生。膀胱或输尿管损伤0例。低压非控压组平均手术时间(44±11)min,出血量(45±10)ml,无电切综合征发生,膀胱或输尿管损伤4例。结论 经尿道前列腺电切中采用低压、控压膀胱冲洗,有手术时间短,副损伤少,并发症低的优点。 相似文献
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目的:探讨妊娠中晚期孕妇血清25羟维生素D[25(OH)D]营养状态对妊娠结局的影响。方法收集中晚期孕妇143例行问卷调查了解生活习惯。应用高效液相色谱‐串联质谱法检测血清25(OH)D ,根据血清25(OH)D水平将受试者分为维生素D充足组(n=32)、欠当组(n=49)、不足组(n=51)及缺乏组(n=11)。采用回顾性分析的方法,对孕妇的妊娠结局进行跟踪随访。结果(1)妊娠中晚期孕妇血清25(O H )D平均值为(22.49±9.51)ng/m L。(2)流产率在充足组、欠当组、不足组、缺乏组分别为3.1%(1/32)、2.0%(1/49)、11.8%(6/51)、36.4%(4/11),胎儿窘迫发生率3.1%(1/32)、8.2%(4/49)、11.8%(6/51)、36.4%(4/11)。维生素D缺乏组流产、胎儿窘迫发生率高于其余各组,差异有统计学意义(P<0.05)。(3)各组细菌性阴道病发生率为0(0/32)、4.1%(2/49)、13.7%(7/51)、27.3%(3/11),维生素D缺乏组、不足组细菌性阴道病发生率高于其余各组,差异有统计学意义( P<0.05)。(4)各组孕妇妊娠周期为(39.48±1.44)、(39.00±1.70)、(38.09±2.01)、(37.29±0.49)周,维生素D不足组、缺乏组孕周较充足组、欠当组明显缩短,差异有统计学意义(P<0.05)。结论妊娠中晚期维生素D缺乏可增加流产、胎儿窘迫、细菌性阴道病的发生率,并显著缩短妊娠周期。 相似文献
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