丹参对大鼠烫伤早期肝组织含水量的影响

目的观察大鼠严重烫伤早期肝组织含水量的变化及丹参对它们的影响。方法建立30%III度烫伤模型,88只Wistar大鼠随机分为正常对照组(A组,8只)、平衡液复苏组(B组,40只)和丹参治疗组(C组,40只)。B、C两组烫伤后立即按Parkland公式腹腔注射平衡液120ml/kg,C组在同样治疗的基础上腹腔注射丹参注射液3ml/kg,B、C两组分别于伤后3,6,12,24,48h测定肝组织含水量。结果与对照组相比较,复苏组肝组织含水量在伤后6h即明显高于对照组,48h达高峰;丹参治疗组在各相应时相点与对应的复苏组比较,两者的变化趋势基本一致,但肝组织含水量均低于复苏组,伤后6h起显著低于复苏组。结论(1)大鼠严重烫伤早期肝组织含水量即明显升高。(2)丹参可使严重烫伤早期肝损伤的肝组织含水量降低,对烫伤早期肝损伤有保护作用。     

腹壁子宫内膜异位症28例临床分析

子宫内膜异位症是生育年龄常见病，分布范围广。腹壁子宫内膜异位症临床少见，多发生于中期妊娠剖宫取胎或足月剖宫产术后。近年来，随着剖宫产率的增加，腹壁子宫内膜异位症逐渐上升。现对我院自1990年1月至2005年7月经手术及病理确诊的腹壁子宫内膜异位症28例，进行回顾性分析报道如下     

急性心肌梗死溶栓血管再通早晚与梗死面积的关系

目的：观察经静脉溶栓治疗急性心肌梗死（AMI）患者，梗死相关血管（IRA）再通早晚与其心肌梗死面积之关系。方法；选取经溶栓后IRA再灌流患者71例，发病2～3周行99mTC－MIBI心肌断层显像测定AMI梗死范围指数，二维超声测定在室射血分数（LVEF）。结果：1）57例距发病≤6hIRA再通患者的梗死范围指数低于14例＞6h组（0．326±0．075比0．373±0．071，P＜0．05）；2）梗死范围指数与LVEF中度相关（r＝0．5139，P＜0．001）。结论：AMI患者静脉溶栓治疗IRA早开通比晚开通者的心梗范围缩小，心功能得到更好保护。     

Guattegaumerine的神经细胞保护及细胞内钙调节作用

目的探讨guattegaumerine(Gua)对H2O2合并血清剥夺诱导的培养大鼠神经细胞的保护作用,以及对H2O2、KCl诱导的大鼠皮质神经元内钙超载和缓激肽诱导的人神经母细胞瘤细胞(SH-SY5Y)内钙超载的影响。方法 H2O2合并血清剥夺诱导培养大鼠皮质神经元损伤,Hoechst33258染色法检测Gua对细胞凋亡的影响;钙荧光探针Furo-2/AM标记细胞,荧光显微图像分析系统和双荧光波长分光光度计检测Gua对细胞内钙浓度的影响。结果 Gua能显著抑制H2O2导致的神经元凋亡、H2O2和KCl引起的培养皮质神经细胞[Ca2+]i升高(P<0.05)和缓激肽所诱导的培养人神经母细胞瘤细胞SH-SY5Y[Ca2+]i升高(P<0.01)。结论 Gua具有一定的神经细胞保护作用,该作用可能与其抑制细胞外钙内流及内质网内钙释放有关。     

虹膜周边切除术47例临床观察

随访观察近五年虹膜周边切除术病人47例78眼。检查、记录其视力、眼压、房角图，对比二小时暗室低头试验前后高低眼压变动下房角变化情况。结果表明：宽基底虹膜周边切除术对急闭临床前期和间歇期，慢闭早期和混合性期，虹膜根赘合并单青均有较好的疗效。提示对虹膜周边切除术后眼压升高及二小时暗室低头试验结果阳性的“残余性”青光眼患者均需作进一步补充性治疗。同时也应避免因选择术式不当造成的“医源性”青光眼。     

Antioxidant and anti-inflammatory activities of the phenolic extracts of Sapium sebiferum (L.) Roxb. leaves

Ethnopharmacological relevance

The leaves of Sapium sebiferum have long been used in Traditional Chinese Medicine (TCM) for the treatment of eczema, shingles, edema, swelling, ascites, scabs, and snakebites, among other maladies.

Aim of this study

The present study aimed to investigate the antioxidant and anti-inflammatory effects of the phenolic extracts of Sapium sebiferum leaves using in vitro and in vivo models.

Materials and methods

The in vitro antioxidant activities of the extracts were measured using common chemical methods (total phenolic content; total flavonoid content; scavenging of DPPH·, ABTS+·, superoxide, and nitrite radicals; reducing power; β-carotene bleaching; and FTC assays). The in vivo topical anti-inflammatory activities were tested using the 12-O-tetradecanoylphorbol-13-acetate (TPA)-induced dermatitis animal model. The SOD and CAT activities and the GSH content of ear tissue were also determined using test kits.

Results

The extracts of Sapium sebiferum leaves exhibited strong in vitro antioxidant activities. They also showed significant (P<0.001) and dose-dependent anti-inflammatory activities in an acute dermatitis model at the doses of 0.03 mg/ear, 0.1 mg/ear, and 0.3 mg/ear. The application of Sapium sebiferum leaf extracts increased the SOD and CAT activities and the GSH content relative to those of the TPA treatment group. The anti-inflammatory effect of the Sapium sebiferum leaf extract was positively correlated with its antioxidant activity.

Conclusion

These results demonstrate that Sapium sebiferum leaf extract is an effective anti-inflammatory agent in the TPA-induced dermatitis model, and its anti-inflammatory effect is related, at least in part, to its antioxidant activity.