排序方式: 共有22条查询结果,搜索用时 31 毫秒
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目的:探讨龙胆紫染色区分存活精子和死亡精子可能性,以提供一种判定精子存活率的新方法,为男性精液检测和质量评价提供依据。方法:采用不同浓度的龙胆紫溶液对精液样本染色,在光学显微镜下观察染色结果,讨论区分存活精子和死亡精子的标准,并计数一定数量的总精子数,计算存活率,并将计算结果与标准方法比较。结果:经过龙胆紫溶液(0.05%,M/V)染色后在显微镜下存活精子呈现淡蓝色,而死亡精子呈现深紫色,计算获得的存活率与伊红染色法(0.5%,M/V)的结果比较差异统计学意义(t=0.862,双侧P=0.403)。结论:一定浓度的龙胆紫染色后能够清楚地区分存活精子和死亡精子,该法获得的精子存活率结果可靠,龙胆紫染色法可以作为一种新的分析精子存活率的方法。 相似文献
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目的探讨腹腔镜胰十二指肠切除术(LPD)的安全性和有效性。方法回顾性分析2014年5~12月于华中科技大学同济医学院附属同济医院胆胰外科完成的60例胰十二指肠切除术患者的围术期临床资料,其中LPD 30例,开腹胰十二指肠切除术(OPD)组30例。比较两组患者围术期结果间的差异。结果 LPD组患者手术时间长于OPD组[(542.6±58.6)min vs(383.1±64.6)min,P0.001)],但术中出血量更少[(190.3±192.0)ml vs(373.3±363.8)ml,P=0.018)],术中输血率更低(30%vs 66.7%,P=0.004),术后胃排空延迟发生率更低(6.7%vs 26.7%,P=0.038),入住科室ICU时间[(2.3±0.9)d vs(5.0±0.9)d,P0.001)]及住院时间[(14.7±1.2)d vs(18.5±2.1)d,P0.001)]更短,肿瘤直径更小[(2.6±0.7)cm vs(3.9±2.4)cm,P=0.009)]。两组患者术后胰瘘、胆瘘、出血、腹腔感染/脓肿、切口感染、二次手术率、总并发症发生率、病死率、手术切缘(R0切除率)、术中切除淋巴结数、阳性淋巴结数、血管浸润率及肿瘤分化程度间比较,差异均无统计学意义。结论 LPD虽手术复杂、难度大,然对经验丰富的胰腺外科医师而言,该术式是安全有效的。 相似文献
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老龙皮经热水提取,乙醇沉淀及进一步精制得LOK(Lobaria kurokauae)多糖,经SephadexG-75柱层析为一组均一多糖,糖含量为82.4%。气相色谱分析含有鼠李糖、阿拉伯糖、木糖、甘露糖、葡萄糖、半乳糖。其克分子比为1.00:1.34:1.12:10.13:6.99:6.47。经Sephadex G-200柱层析测定,平均分子量为1.5×10~4。经高碘酸氧化,Smith降解,有甲酸、丙三醇、丁四醇生成,红外光谱在842cm~(-1)有吸收,说明LOK多糖结构主要以α(1→4)、a(1→6)苷键连接而成的杂多糖。 相似文献
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Zhuoma Dawa Yang Bai Yan Zhou Suolang Gesang Ping A Lisheng Ding 《Journal of natural medicines》2009,63(3):327-330
The chemical constituents of the traditional Tibetan medicine of Saussurea medusa Maxim. (Compositae) were investigated and a new flavonoid glucoside, together with 14 known compounds, was isolated. The
structure of the new compound was established as 6″-O-crotonoylhomoplantaginin by using one- and two-dimensional nuclear magnetic resonance spectroscopy and mass spectrometry
analyses. 相似文献
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通过维医药辨证分型,对辨证治疗冠心病心绞痛的疗效作出了综述,指出其仍需要临床分析并加以报道。 相似文献
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Dawa Z Zhou Y Bai Y Gesang S Bai B Ding L 《Journal of pharmaceutical and biomedical analysis》2008,48(4):1076-1081
An improved HPLC-DAD-ESI-MS(n) method has been developed to simultaneously quantify eight major compounds in Saussurea tridactyla Sch.-Bip. ex Hook. f. which has long been used as a traditional Tibetan medicine. This method was validated to be sensitive, precise and accurate with the LODs of 0.11-5.01 microg/ml, the overall intra-day and inter-day variations less than 2.70%, and the overall recovery over 98.0%, respectively. The correlation coefficients (r(2)) of the calibration curves were higher than 0.991. This newly established method was successfully applied to reveal the difference in the chemical profiles and contents of these analyses in S. tridactyla from different localities. In addition, by comparison UV and MS spectra with those of authentic compounds and literatures, a total of fourteen peaks were identified. It can be concluded that this method was effective to ensure the safety and efficacy consistency of S. tridactyla, and can be applied to other traditional Tibetan medicinal plants from different resources in Tibet. 相似文献
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孕中期唐氏筛查及诊断的结果分析 总被引:1,自引:0,他引:1
目的 探讨女性妊娠中期唐氏筛查对宫内诊断的临床价值以及对不同年龄段的异常率差异分析.方法 采用磁微粒化学发光法对14 742例妊娠中期女性进行血清甲胎蛋白(AFP)、游离β-人绒毛膜促性腺激素(f-β-HCG)和游离雌三醇(uE3)检测,并计算风险值.结果 621例孕妇筛查结果为高风险,阳性率为4.21%,检出NTD高风险孕妇378例,筛查阳性率为1.40%,检出爱德华综合征(Es)高风险孕妇36例,筛查阳性率为0.24%(36/14 742),在不同年龄的高风险分组中,大于或等于35岁组与其他组别作统计比较,唐氏综合征(Ds)风险率差异有统计学意义(P<0.05),各年龄组胎儿神经管缺陷(NTDs)风险率差异无统计学意义(P>0.05).结论 产前筛查对于宫内诊断具有重要的临床价值,高风险对产前诊断起重要的指导作用. 相似文献