穿心莲片的工艺改进

采用浸膏(比重:95℃,1.30)与辅料直接混合制粒法生产的穿心莲片,具有产品生产周期缩短,成本降低,质量和收率提高等优点,并通过留样观察,证明此法是可行的。     

具有降低血中过氧化脂质的汉方方剂

活性氧被认为是很多疾病发病的原因之一,近年来不断引起人们的重视,因而本文采用活性氧增加,血中过氧化脂质上升作为实验模型,观察汉方方剂在体内抗氧化能力。当用20～25gddy系雄性小鼠,12h禁食后,给所试药物,1h后,iv内毒素30mg/kg,又5h从眼底取血,用TBA荧光法测定过氧     

溃疡局部切除治疗消化性溃疡穿孔和出血81例分析

目的：探讨溃疡局部切除治疗消化性溃疡穿孔和出血的可行性和临床意义。方法：回顾性分析我院收治的81例消化性溃疡局部切除患者的临床资料。结果：无溃疡再穿孔和再出血病例,1例死于肺部感染,溃疡复发7例,复发伴出血2例,复发病例都经非手术治疗治愈。结论：溃疡局部切除治疗消化性溃疡穿孔和出血,具有创伤小、操作简单、对患者打击小、并发症少等优点,同时维持了患者胃的正常容量和生理解剖通路,值得推广。     

游离型精索睾丸扭转合并嵌顿疝1例分析

对游离型精索睾丸扭转合并嵌顿疝1例分析如下。 1病历摘要 男,15岁。因左侧腹股沟区包块伴疼痛5h而入院。入院前5h,患者因左侧腹股沟区疼痛而于夜间睡眠时被痛醒,为持续性胀痛,阵发性加重,伴有恶心、呕吐,局部可扪及包块。查体：急性痛苦病容,左侧腹股沟区可扪及一3．0cm×2．0cm的包块,明显压痛,包块不能还纳入腹腔,左侧睾丸较对侧偏大,有触痛。诊断考虑为左侧腹股沟疝嵌顿,急诊手术治疗。     

乳头巨大软纤维瘤一例

患者,女,40岁。因发现左乳头包块30年入院。查体:左侧乳房因包块牵拉而下垂。且皮肤浅表血管清晰可见。与对侧相比左侧乳房浅表静脉明显扩张。乳头被拉长,约5．0 cm,表面有少许乳白色的分泌物。乳头悬垂一带蒂的包块,大小约11cm×6cm×5cm,分叶状,柔软,表面不光滑,有直径约0．2～0．3 cm结节,呈“桑椹”样改变(图1)。左侧乳房未扪及包块,同侧腋窝、锁骨上无肿大的淋巴结。右侧乳房无异常发现。入院诊断为左乳头软纤维瘤．行左乳头肿物单纯切除术,术后一月随访左乳头无缺血坏死表现。病理检查结果证实为软纤维瘤(图2)。     

糖基化终末产物对人外周血内皮祖细胞生物学特性的影响

Objective In addition to be involved in the angiogenesis, endothelial progenitor cells (EPCs) have roles in endothelium repairing, wound healing, and for protecting blood vessels from restenosis, Advanced glycation end products (AGEs) facilitate the development and progression of atherosclerosis, diabetes associated vascular complications and uremia through various mechanisms such as damaging the endothelium, promoting leukocyte adhension, increasing the aggregation of platelets, and stimulating the proliferation of vascular smooth muscles. This study was designed to explore whether AGEs have effects on biological characteristics of EPCs in cultured human peripheral blood cells. Methods Total mononuclear cells (MNCs), isolated from human peripheral blood by density gradient centrifugatian and adherence cells filtration, were incuba-ted in fibronectin-coated culture dishes. Endothelial cells were identified by means of the adsorption of ulex eurepaeus-aggluti-nin- Ⅰ (UEA- Ⅰ) labelled with fluorescein isothiacyanate (FITC) and Dil-acLDL internalization. Four days later,various con-centrations of AGEs were added to the adherent cells and remained for48 hours. MTT assay and Boyden chamber were used for observing the proliferation and migration of EPCs. Human fibronectin was used to examine the adhesion ability of EPCs. Apop-tosis was induced in the EPCs with formaldehyde and Dnase Ⅰ as a positive control group. Annexin V-FITC/PI and TUNEL method of flow cytometry were used for evaluating the effects of AGEs on the rate of apeptosis in the EPCs. Results AGEs at high concentration decreased the number of EPCs independently (P < 0.01) ; reduced the proliferation (P < 0.01), migration (P<0.001) and adhesive capacity (P<0.05) of EPCs significantly,as well as increasing the apoptasis rate of EPCs in the early stage (P < 0.001). Conclusion AGEs may have adverse effects on EPCs from cultured human peripheral MNCs, such as decreasing their numbers and impairing their functions.     

新生儿先天被动免疫性血小板减少性紫癜一家二例

例1 男,生后28小时,生时头顶部有一血肿,生后半小时血肿增大,颜面有小出血点,2小时全身皮肤出现出血点,20小时全身有小抽动(考虑为颅内出血所致),查血小板数2.5万。患儿为足月顺产,其母曾患原发性血小板减少性紫癜,分娩前血小板计数3.8万。体检:体温37℃,脉搏144次,体重2750g     

B超在急性阑尾炎中的诊断价值探讨

目的:探讨B超对急性阑尾炎的诊断价值.方法:回顾性分析450例临床拟诊急性阑尾炎患者的超声、手术及病理资料.结果:B超诊断急性阑尾炎符合率90%以上,炎症较轻的急性单纯性阑尾炎的诊断符合率为85.7%,化脓性、坏疽性阑尾炎及阑尾周围脓肿的符合率达93%以上.结论:B超对急性阑尾炎检出率较高,具有方便、快捷、无创、可反复...     

糖基化终末产物对人外周血内皮祖细胞生物学特性的影响

目的 观察糖基化终末产物(AGEs)对体外培养的人外周血内皮祖细胞(EPCs)生物学特性的影响.方法 以密度梯度离心法获取人外周血单个核细胞(MNCs),由激光共聚焦显微镜鉴定FITC-UEA-1和Dil-acLDL双染色阳性细胞为正在分化的EPCs.加入不同浓度AGEs培养48h,然后分别采用MTT法、Boyden小室测定来观察EPCs的增殖、迁移能力;以人纤维连接蛋白(hFN)检测EPCs的黏附能力;分别采用甲醛和Dnase Ⅰ诱导EPCs凋亡作为阳性对照组,用AnnexinV-FITC/PI和TUNEL法流式细胞仪检测AGEs对EPCs凋亡率的影响.结果 高浓度AGEs可减少EPCs贴壁细胞数量(P<0.01),明显减弱EPCs的黏附(P<0.01)、增殖(P<0.001)、迁移能力(P<0.05),提高EPCs的早期凋亡率(P<0.001).结论 AGEs可减少EPCs的数昔并使EPCs的功能受损.     

内皮祖细胞功能失调与糖尿病血管病变

血管病变为糖尿病常见的并发症,严重影响了患者生活质量。糖尿病的血管修复能力降低,主要表现在创伤愈合能力受损和缺血时侧支循环形成减少,而内皮祖细胞(EPC)在这些过程中有重要的调节作用[1]。EPC在人和动物模型已经显示能促进血管新生和再内皮化,表明它在维持内皮完整性中有