前列腺癌基因诊断芯片的临床应用

目的应用基因芯片诊断前列腺癌.方法提取前列腺癌及正常前列腺组织总DNA并纯化mRNA,以包含了9个前列腺癌相关的特异基因和1个参照基因的xy检测系统cDNA临床芯片,对前列腺癌及正常前列腺组织的基因表达谱进行分析.结果9个前列腺癌相关基因检测中癌与正常组织存在显著差异,其中显著上调的有7条;显著下调有2条.结论前列腺癌临床基因诊断芯片作为前列腺癌分子水平的诊断的方法,有望提高前列腺癌的检出率.     

应用输尿管镜放置双J管早期治疗输尿管阴道瘘

Objective To evaluate the clinical effect of placing double J stent using a ureteroscope in early managing ureterovaginal fistula.Methods Twenty-eight patients cases with ureterovaginal fistula from 2002 to 2008 were treated early with placing double J stent using a ureteroscope and the clinical data were reviewed.Results Twenty-two of 28 cases were treated and double J stent was placed in them by a uretero-scope and 75% (21/28)of cases were cured.Four of 21 cases were treated twice by a ureteroscope and were cured finally.7 cases with failure ureterovaginal treatment underwent ureterocystostomy and were cured.The follow-up from 6 months to 33 months (average 10.1±6.4 months)showed that all of the 28 cases had been cured and had no urinary fistula.Conclusion Placing double J stent using a ureteroscope is the first choice of operative procedure for the early treatment of ureterovaginal fistula.     

应用输尿管镜放置双J管早期治疗输尿管阴道瘘

Objective To evaluate the clinical effect of placing double J stent using a ureteroscope in early managing ureterovaginal fistula.Methods Twenty-eight patients cases with ureterovaginal fistula from 2002 to 2008 were treated early with placing double J stent using a ureteroscope and the clinical data were reviewed.Results Twenty-two of 28 cases were treated and double J stent was placed in them by a uretero-scope and 75% (21/28)of cases were cured.Four of 21 cases were treated twice by a ureteroscope and were cured finally.7 cases with failure ureterovaginal treatment underwent ureterocystostomy and were cured.The follow-up from 6 months to 33 months (average 10.1±6.4 months)showed that all of the 28 cases had been cured and had no urinary fistula.Conclusion Placing double J stent using a ureteroscope is the first choice of operative procedure for the early treatment of ureterovaginal fistula.     

应用输尿管镜放置双J管早期治疗输尿管阴道瘘

Objective To evaluate the clinical effect of placing double J stent using a ureteroscope in early managing ureterovaginal fistula.Methods Twenty-eight patients cases with ureterovaginal fistula from 2002 to 2008 were treated early with placing double J stent using a ureteroscope and the clinical data were reviewed.Results Twenty-two of 28 cases were treated and double J stent was placed in them by a uretero-scope and 75% (21/28)of cases were cured.Four of 21 cases were treated twice by a ureteroscope and were cured finally.7 cases with failure ureterovaginal treatment underwent ureterocystostomy and were cured.The follow-up from 6 months to 33 months (average 10.1±6.4 months)showed that all of the 28 cases had been cured and had no urinary fistula.Conclusion Placing double J stent using a ureteroscope is the first choice of operative procedure for the early treatment of ureterovaginal fistula.     

Madigan术与传统开放术式治疗前列腺增生症术后并发症的比较

目的：比较Ｍａｄｉｎｇａｎ术与传统术式治疗前列腺增生症术后并发症。方法：以统计学方法比较两种术式中手术时间，出血量及术后各种并发症的发生率。结果：Ｍａｄｉｇａｎ手术组病人无论在术中还是在术后并发症的发生率均低于传统开放手术组。结论：Ｍａｄｉｇａｎ术为前列腺增生症病人提供了一种安全、有效、经济的手术方法。     

骨盆骨折并后尿道损伤的腔内与开放治疗疗效比较

目的：探讨骨盆骨折并后尿道损伤腔内治疗与开放手术治疗的疗效比较，方法：93例骨盆骨折并后尿道损伤病人中45例进行开放性后尿道端端吻合术，48例行腔内治疗术，术后根据排尿功能恢复及并发症发生率对疗效进行评估比较，结果：腔内治疗病人治疗有效率达到或接近开放手术组，分别为88．5％与83．3％，并发症发生率腔内治疗组较低，结论：腔内手术为骨盆骨折并后尿道损伤提供了一种有效，安全的外科治疗方法。     

腔内与开放术治疗骨盆骨折并后尿道损伤的疗效—成本分析

目的探讨骨盆骨折并后尿道损伤腔内与开放手术治疗的疗效一成本关系.方法93例中45例进行开放性后尿道端端吻合术48例行腔内治疗术,对其疗效评估及医疗成本分析.结果腔内治疗组有效率达到或接近开放手术组,分别为88.5%与83.3%,并发症发生率腔内治疗组较低,医疗费用方面则明显低于开放手术组,平均分别为(4*!978±194.2)与(6*!796±251.8)元.结论腔内手术为骨盆骨折并后尿道损伤提供了一种有效、安全、经济的外科治疗方法.     

Ha-ras mRNA与端粒酶在人膀胱肿瘤组织中的表达

目的　探讨人膀胱癌中Ha rasmRNA及端粒酶的表达与肿瘤临床指标的关系。方法采用原位杂交及银染端粒重复序扩增程序 (TRAP)反应的方法对 91例膀胱癌标本进行Ha rasmRNA及端粒酶活性的表达检测。结果　 91例膀胱癌标本中Ha rasmRNA与端粒酶活性检出率为 73 %与 92 % ,两者阳性率及阳性强度与肿瘤的恶性程度呈正相关 ,在不同临床分期中两者表达差异无显著性 (P >0 .0 5 )。结论　Ha ras癌基因与端粒酶是参与肿瘤的发生并影响肿瘤发展的重要因素 ,有望成为判断肿瘤恶性程度、自然病程及预后的评估指标。     

前列腺增生与前列腺癌中KLK11/TMPRSSmRNA表达比值

目的 以实时荧光定量PCR技术测定前列腺增生(BPH)与前列腺癌(PCa)组织标本KLK11/TMPRSS mRNA比值,探讨KLK11/TMPRSS比值在前列腺癌诊断的特异性意义.方法 通过实时荧光定量PCR对23例PCa、37例BPH及3例正常前列腺组织KLK11/TMPRSS的表达,比较其在PCa与BPH中组织定量的差异.结果 BPH与PCa组织KLK11/TMPRSS mRNA的定量表达值分别为2.264±0.460与5.905±0.780,差异有统计学意义(P＜0.05).结论 实时荧光RTPCR定量检测KLK11/TMPRSS mRNA为前列腺癌的诊断提供了可靠的辅助指标.     

慢性前列腺炎三种药物疗法的费用效果分析

目的评价吾真宁、可乐必妥、阿奇霉素(泰力特)3种治疗慢性前列腺炎药物经济学效果。方法对75例慢性前列腺炎病人分别应用吾真宁、可乐必妥、泰力特治疗，运用药物经济学的成本-效果分析方法进行评估。结果无论是症状消失还是体征消失的效果，吾真宁组的成本效果比均低于可乐必妥及泰力特组。结论吾真宁为慢性前列腺炎患者提供了一种有效、安全、经济的口服药物。