ACTIVIT OF HUMAN PLACENTAL GAMMA GLOBULININ BLOCKING IMMUNE FUNCTIONS IN VITRO AND IN ABROGATING THE XENOGENEIC, LOCAL GRAFT-VERSUS-HOST-REEACTEON

In order to understand the mechanism of immunosuppression caused by infusion of placental gamma globulin (PGG) in patients with renal allografts, rheumatoid arthritis, and graft – versus –host disease (GCHD) following bone marrow transplantation (BMT) ,we have examined the effect of PGG in vitro and in a model of the xenogeneic , local graft –ver- sus – host reaction (LGVHR) .PGG inhibited lymphocyte proliferation in mixed lymphocyte cultures (MLC) (P<0.005) and depressed interleukin -2 (IL-2) levels in such cultures at 72 hours (P<0.01) . In contrast phytohemagglutinin (PHA) –and pokeweed mitogen (PWM) –induced T and B lymphocyte blastogenesis was not affected by such PGG treatment .PGG treatment .PGG neither decreased the [3H] TdR pulse incorporation in unstimulated lymphocytes nor affected cell viability .Cell cycle analysis by flow cytometry showed that PGG reduced the percentage of cells in S and G2, M phases during the MLC, but did not alter cell cycling during PWM-stimulated proliferation . An immunosuppressive effect of PGG on the LGCHR was tested in a model of intracutaneous transplantation of PGG –treat human lymphocytes into cyclophosphamide – immunosuppressed rats. Lymphoprep – separated human tonsillar lymphocytes were incubated with RPMI-1640 buffer containing：（1）PGG,4mg/ml,(2) human plasma albumin,4mg/ml,(3)mitomycin-C,25ug/ml, or (4) no additive. Cell of each preparation (3x107cells in 0.1ml) were injected intracutaneously into cyclophosphamide-treated male rats at separate abdominal locations. A fifth site received only the buffer solution. Five days after injection of cells ,each rat received [125 I]IUdR (10uCi) intraperitoneally and was killed after 5 hours. For each site of injection, the diameters of induration were measured and 125 I was counted . There was no difference between buffer – treated and a ibumin – treated groups either in the diameter of the area of induration (t=0.66;P>0.5)or in radioactive counts(t=0.22;P>0.05).In the PGG –treated group, the induration and radioactivity measurements were significantly less than in control groups (t=3.72 and P<0.1;t=2.62 and P<0.02,respectively ) Cytophilic antibodies in PGG were thought to inhibit an early phase of T cell activation, and not to be cytotoxicity .In the LGVHR, the immune response might be abrogated either by immuno- regulatory suppression of T cell function or by toxicity to the infused lymphoid cells. For some clinical purposes, immuno- modulating, human antibodies might be preferred to murine, monoclonal antibodies.     

HL-60细胞体外分化过程中表面抗原的改变

人早幼粒白血病细胞株(HL-60)在体外分别与1,25二羟维生素D3(1,25(OH)_2D_3)、维甲酸、二甲基亚砜(DMSO)和TPA共同培养5天后,在上述各分化诱导剂的作用下可向单核-巨噬细胞或粒细胞分化。在其分化前后,用抗单核-巨噬细胞或粒细胞的各种单克隆抗体进行间接免疫荧光检查,以比较诱导分化的白血病细胞与处于相应分化阶段的正常细胞抗原性是否相同。结果表明,TPA和1,25(OH)_2D_3(10~(-8)M)可诱导单核-巨噬细胞分化。组化分析证明,ANAE中度或强阳性的细胞经诱导培养后由原来的8％升至73％。但是,经1.25(OH)_2D_3诱导的细胞形态类似单核细胞,可与单克隆抗体FMC17及FMC32有较强的结合     

胎盘血丙种球蛋白的研究和应用

按照以往资料,从胎盘血提取出的丙种球蛋白(PGG)与从静脉血提取者相比,具有若干独特之处,即呈现出较强的体外免疫抑制效应,抗细胞抗体活性和阻断 EA 花结形成的能力。一些研究者将其应用于临床实践,也获得一定的效果。PGG 的这些作用不能用被动抗体增强抵抗力来解释。一、PGG 的免疫学特征1.体外免疫抑制效应实验表明,PGG 能够显著抑制单向和双相混合淋巴细胞培养(MLC),并表现出剂量依赖关系.在多数报告中,仅用数十至数百     

正常人骨髓红系集落的琼脂培养

培养正常人造血组织的红系集落惯用甲基纤维素(MC)或血浆凝块法。用MC的主要弊端是不能制备用于细胞学或细胞化学分析的永久性标本。本研究的基本目的是:确定琼脂是否象MC一样有效地支持正常人红系细胞的体外生长,其次是通过缩小培养体系来节省红细胞生成素(Epo)。 MC培养依据Guilbert和Iscove的方法。其培养体系为:含终浓度30%的胎牛血清(FBS)的Iscove改良的Dulbecco培养基(IMDM)。Epo的终浓度为2u/ml,细胞浓度为2×10~5/ml。全部实验用同一批     

蒙药芯芭粉末及茎横切片的显微鉴别

目的:对蒙药芯芭属两种植物达乌里芯芭和蒙古芯芭的粉末和茎横切片进行显微观察,为今后对蒙药芯芭的进一步研究提供参考。方法:用显微镜观察两种芯芭粉末和茎的横切面的特征。结果:两种芯芭粉末均可见非腺毛、腺毛、纤维、环纹导管、孔纹导管、不定式气孔、花粉粒、棕色块和果实片段。两种芯芭茎的横切面均可见表皮、皮层、初生韧皮部、束中形成层、初生木质部、髓、非腺毛。二者的显微区别主要为非腺毛与腺毛数量的多少。结论:以上显微特征对蒙药芯芭有鉴别意义。     

构建药学专业“一体化、阶梯式、多层次”实践教学体系的探索与实践

在分析国内外药学教育模式现状的基础上,结合本院特点,提出药学专业"一体化、阶梯式、多层次"实践教学新模式,介绍了在改革教学内容、教学模式、教学方法、考核评价机制等基础上对构建药学专业"一体化、阶梯式、多层次"实践教学体系的探索。     

中药党参贮藏方法的观察

目的探求贮藏党参药材的最佳方法。方法采用酒精封存法、石灰封存法、低温干燥贮存法、常温贮存法观察中药党参的贮存效果。结论酒精封存法和低温干燥法是保证党参品质的良好贮存方法。     

硫代硫酸钠溶液与盐酸溶液配用治疗疥疮疗效分析

作者在边防部队工作期间从事药剂工作,常见许多官兵患有疥疮且相互传染.在临床上常选用硫磺软膏外涂,但其油性较强,使用时有很多不便.我们采用40%硫代硫酸钠溶液与4%盐酸溶液配合进行治疗,疗效满意,现将结果报道如下.     

成人而不是儿童急性淋巴细胞白血病细胞产生T细胞生长因子

对白血病细胞的广泛研究表明,不同类型的急性淋巴细胞白血病(ALL)相当于正常淋巴细胞分化的不同阶段。在儿童ALL,常有ALL共同抗原(CALLA )的ALL细胞可能是B前体细胞。作者研究了7例儿童和3例成人ALL细胞T细胞生长因子(TCGF)的生成及其对TCGF的反应。采用单克隆抗体间接免疫荧光法用流式细胞光度计(荧光激活细胞分类器,FACS)分析细胞表型,证明在这些儿童ALL中有5例子-ALL,4例非T非B型(儿童和成人ALL各2例),裸细胞-ALL 1例(成人)。3例成人白血病细胞均为TdT阳性。正常或病人细胞的TCGF生成,按Gillis法测定;ALL细胞对TCGF的反应则以加入外源性TCGF培养3天后测定~3H-TdR的掺入率为指标。结果表明:不论表型如何,儿童ALL细胞均不能产生TCGF;相反,所有3例成人ALL的细胞都可以生成显著活性的TCGF。提示,TCGF的产生只限于