《实验诊断学》实验课教学模式新探索

《实验诊断学》在基础医学和临床医学中发挥重要的桥梁作用.近年来,分子生物学、免疫学、遗传学等相关学科的高速发展极大推动了检验医学的发展,也推动了实验诊断学的发展.……     

焦虑障碍的催产素-HPA轴异常及针刺干预机理探析

新近研究发现,催产素(Oxytocin,OT)系统的激活可抑制下丘脑-垂体-肾上腺轴(hypothalamo-pituitary-adrenalaxis,HPA轴)亢进而发挥抗焦虑效应;OT-HPA轴活性异常与焦虑障碍的发生密切相关。针刺对焦虑障碍有良好的调节作用。针刺调节焦虑障碍与OT-HPA轴是否相关,尚未见文献研究与分析报道。据此,本文拟从OT、HPA轴和OT-HPA轴与焦虑障碍,以及针刺调节OT-HPA轴这四方面,分析探讨针刺治疗焦虑障碍的OT-HPA轴调节机理,从而为针刺治疗焦虑障碍的新机制研究提供思路。     

CD62P和CD63在糖尿病肾病的临床应用

美国糖尿病联合会的新分类法认为2型糖尿病(T2DM)是最常见的糖尿病。笔者应用流式细胞仪及特定蛋白分析仪检测T2DM患者外周血血小板的活化程度及尿中微量球蛋白(β2M)的表达，探讨血小板表面α-颗粒膜蛋白(CD62P)和血小板溶酶体α-颗粒膜蛋白(CD63)对早期诊断T2DM肾损害的临床价值。     

高敏C反应蛋白检测在脑血管疾病中的意义

目的:探讨血清高敏C反应蛋白(hight-sensitive C-reactive protein,hs-C R P)与脑血管疾病的关系。方法:采用免疫散射速率比浊法,检测60例脑血管疾病患者和25例健康体检对照者血清hs-C R P水平。结果:18例短暂脑缺血发作(TIA)组hs-C R P为(1.94±1.40)m g/L,25例脑梗死组为(15.51±6.91)m g/L,17例脑出血组为(11.95±4.88)m g/L,20例正常对照组为(1.09±0.66)m g/L,脑梗死组、脑出血组与对照组比较血清hs-C R P水平升高,差异均具有显著性(P<0.001);TIA组与对照组比较差异无显著性(P>0.05)。结论:血清hs-C R P水平与脑血管疾病密切相关,可预测脑血管疾病的危险性,并可作为监测病情和预后判断的指标。     

恶性肿瘤患者血清Kappa与Lambda轻链检测及临床意义

Objective To investigate the abnormal expressions of immunoglobulin light chains kappa(Igκ)and Lambda(Igλ)in malignant tumors.Methods The concentrations of the Igκ and Igλ in serum were measured by rat nephelometry in 60 cases of malignant tumors and 15 cases of healthy people.Results The liver tumor patients had a higher concentration of Igκ and Igλ in serum than normal people(P＜0.01).There wre not significant differences between the levels of Igκ,Igλ and κ/λ ratio in the stomach tumor,lung tumor,proctologic/colonic tumor,mammary tumor,oesophagus tumor and normal peoples(P＞0.05).Conclusion The measurment of Igκ,Igλ and κ/λ ratio in serum would be helpful for the diagnosis of the liver tumor.By continous observation with Igκ,Igλ and κ/λratio in serum could predict the development of disease.     

恶性肿瘤患者血清Kappa与Lambda轻链检测及临床意义

Objective To investigate the abnormal expressions of immunoglobulin light chains kappa(Igκ)and Lambda(Igλ)in malignant tumors.Methods The concentrations of the Igκ and Igλ in serum were measured by rat nephelometry in 60 cases of malignant tumors and 15 cases of healthy people.Results The liver tumor patients had a higher concentration of Igκ and Igλ in serum than normal people(P＜0.01).There wre not significant differences between the levels of Igκ,Igλ and κ/λ ratio in the stomach tumor,lung tumor,proctologic/colonic tumor,mammary tumor,oesophagus tumor and normal peoples(P＞0.05).Conclusion The measurment of Igκ,Igλ and κ/λ ratio in serum would be helpful for the diagnosis of the liver tumor.By continous observation with Igκ,Igλ and κ/λratio in serum could predict the development of disease.     

前带反应伴溶血现象致ABO定型困难一例

前带反应伴溶血现象致ＡＢＯ定型困难一例姚弘（江苏吴县红十字医院，江苏吴县２１５１２８）王雪明（苏州医学院附属一院）最近，我们遇见一例因ＩｇＭ抗－Ｂ凝集效价较高，同时伴有溶血现象，致ＡＢＯ反定型困难一例，现报道如下：１病例摘要患者王某，女性，４０岁，于...     

WNT信号通路在KRAS基因突变型结直肠癌中的作用

目的:探讨WNT信号通路在KRAS基因突变型结直肠癌的作用.方法:收集206例郑州大学第一附属医院结直肠癌患者的临床病理资料及其新鲜组织,采用qRT-PCR方法检测WNT信号通路相关蛋白β-catenin和Cyclin D1 mRNA的表达.将KRAS基因突变的结直肠癌细胞SW480分为β-catenin siRNA组...     

恶性肿瘤患者血清Kappa与Lambda轻链检测及临床意义

Objective To investigate the abnormal expressions of immunoglobulin light chains kappa(Igκ)and Lambda(Igλ)in malignant tumors.Methods The concentrations of the Igκ and Igλ in serum were measured by rat nephelometry in 60 cases of malignant tumors and 15 cases of healthy people.Results The liver tumor patients had a higher concentration of Igκ and Igλ in serum than normal people(P＜0.01).There wre not significant differences between the levels of Igκ,Igλ and κ/λ ratio in the stomach tumor,lung tumor,proctologic/colonic tumor,mammary tumor,oesophagus tumor and normal peoples(P＞0.05).Conclusion The measurment of Igκ,Igλ and κ/λ ratio in serum would be helpful for the diagnosis of the liver tumor.By continous observation with Igκ,Igλ and κ/λratio in serum could predict the development of disease.     

PCR法检测中国结直肠癌患者MSI状态的研究

目的 开发一种灵敏度、特异性、准确性的检测微卫星不稳定(MSI)状态的PCR扩增方法,以期为中国人群MSI检测位点提供最佳临床数据.方法 选取郑州大学第一附属医院2017年至2019年收治的201例结直肠癌患者样本,利用PCR法检测5个标准MSI检测位点(BAT25,BAT26,D2S123,D5S346和D17S25...