铁调节蛋白2基因多态性与阿尔茨海默病及血管性痴呆的相关分析

Objective To evaluate the association of 2616c/T polymorphism in iron regulatory protein 2(IRP2)gene with Alzheimer disease(AD)and Vascular dementia(VD).Methods In this study,281 patients with AD,60 with VD,and 285 normal aged were recruited.The 2616C/T polymorphism in IRP2 gene was analyzed by using polymerase chain reaction-restriction fragment length polymorphism.And the cognitive function was assessed with the Mini-Mental State Examination(MMSE).Results (1)No significant difieFences were demonstrated in IRP2 genotype or allele frequencies between AD patients and controls(χ2=2.46,P=0.292;χ2=2.17,P=0.141 respectively).However,when AD patients were stratified by disease severity.the frequency of T allele carriers in the moderate to severe AD patients was 78.0%,significantly higher than that in controls(69.8%;χ2=4.106,P＜0.05).Logistic regression analysis demonstrated that the age-,sex-and ApoE-adiusted OR of modcrate to severe AD patient with T allele was 1.62(95% CI=1.03-2.54).The frequency of T allele carriers or T allele in VD patients was higher than that of controls,but the difference was not statistically significant(P＞0.05).(2)The frequency of tit genotype or T allele in the moderate to severe AD patients was significantly higher than that in mild AD patients(25.8%vs.12.5%,χ2=5.477,P＜0.05;51.9%vs.40.3%,χ2=5.803,P＜0.05 respectively).(3)MMSE scores of the AD patients with TT genotype was significantly lower than ones with CC or CT genotype(P=0.028;P=0.014 respectively).Conclusion The 2616C/T polymorphism in the IRP2 gene is possibly associated with moderate to severe AD.but not associated with VD.And the TT genotype may be a risk factor for cognitive impairment of patients with AD in Chinese Han.     

铁调节蛋白2基因多态性与阿尔茨海默病及血管性痴呆的相关分析

Objective To evaluate the association of 2616c/T polymorphism in iron regulatory protein 2(IRP2)gene with Alzheimer disease(AD)and Vascular dementia(VD).Methods In this study,281 patients with AD,60 with VD,and 285 normal aged were recruited.The 2616C/T polymorphism in IRP2 gene was analyzed by using polymerase chain reaction-restriction fragment length polymorphism.And the cognitive function was assessed with the Mini-Mental State Examination(MMSE).Results (1)No significant difieFences were demonstrated in IRP2 genotype or allele frequencies between AD patients and controls(χ2=2.46,P=0.292;χ2=2.17,P=0.141 respectively).However,when AD patients were stratified by disease severity.the frequency of T allele carriers in the moderate to severe AD patients was 78.0%,significantly higher than that in controls(69.8%;χ2=4.106,P＜0.05).Logistic regression analysis demonstrated that the age-,sex-and ApoE-adiusted OR of modcrate to severe AD patient with T allele was 1.62(95% CI=1.03-2.54).The frequency of T allele carriers or T allele in VD patients was higher than that of controls,but the difference was not statistically significant(P＞0.05).(2)The frequency of tit genotype or T allele in the moderate to severe AD patients was significantly higher than that in mild AD patients(25.8%vs.12.5%,χ2=5.477,P＜0.05;51.9%vs.40.3%,χ2=5.803,P＜0.05 respectively).(3)MMSE scores of the AD patients with TT genotype was significantly lower than ones with CC or CT genotype(P=0.028;P=0.014 respectively).Conclusion The 2616C/T polymorphism in the IRP2 gene is possibly associated with moderate to severe AD.but not associated with VD.And the TT genotype may be a risk factor for cognitive impairment of patients with AD in Chinese Han.     

α2-巨球蛋白基因I1000V多态性与散发阿尔茨海默氏病的关联研究

目的近年来有研究发现α2-巨球蛋白基因(α2-macroglobulin，A2M)Ile1000Val多态与阿尔茨海默氏病(Alzheimer’s disease，AD)发病有关联，但也有相悖的研究结果报道。因此．我们利用较大的样本，观察了A2M基因Ile1000Val多态在广州及成都地区汉族老年人中的分布，并探讨其与散发AD的相关性。方法以广州地区257例散发AD患者和242名正常老年人、成都地区112例散发AD患者和113名正常老年人为对象进行病例一对照研究。用聚合酶链反应一限制性片段长度多态性方法分析A2M基因11000V多态性和载脂蛋白E基因(apolipoprotelnE，apoE)多态性。结果(1)在两地合并样本中，AD患者与对照组中等位基因A2M-1000V的频率分别为7．7％与8．7％，广州与成都地区AD患者与对照组中A2M基因I1000V多态的分布差异无统计学意义。(2)散发AD无论按是否伴有apoE—ε4或按发病年龄分成不同亚组后，A2M基因I1000V多态的分布在病例组与对照组之间差异无统计学意义。结论广州与成都汉族人群中A2M基因I1000V多态与散发AD不具有关联。     

SLC1A1基因多态性与中国汉族人群强迫症的相关性

目的：探讨谷氨酸转运体基因（SLC1A1）与中国汉族人群强迫症（OCD）的关系。方法：采用病例对照研究,以125例患者（OCD组）和125例正常人（对照组）作为研究对象,检测SLC1A1基因的3个位点（rs301430、rs301434、rs301979）,分析这些位点等位基因、基因型分布,以及单倍体与OCD之间的关系。结果：3个位点的等位基因和基因型频率分布在OCD组和对照组中的差异无统计学意义（P＞0.05）。经性别分层分析,相较于对照组,女性患者rs301979位点等位基因和基因型频率分布与对照组差异有统计学意义（χ2=7.875,P=0.019;χ2=7.331,P=0.007）,而男性患者中所有位点的等位基因和基因型频率分布差异均无统计学意义（P＞0.05）。三位点单倍体T-A-G在2组中的差异有统计学意义（χ2=6.206,P=0.013）。结论：SLC1A1基因rs301979位点可能是女性OCD的易感基因位点,rs301430、rs301434、rs301979的单倍体T-A-G可能与中国汉族人群OCD发病相关。     

成都地区≥55岁人群抑郁障碍患病率调查

目的调查成都地区≥55岁人群抑郁障碍的患病率。方法采用分层随机整群抽样方法,从成都市所辖19个区、市、县抽取样本,调查分为筛查、复查诊断2个阶段。筛查用一般情况量表、中文版简易精神状态检查表(MMSE)、日常生活功能量表(ADL)、流调用抑郁自评量表(CESD)、体格检查及神经系统检查表,复查诊断用DSMⅢR定式临床检查患者版(SCIDP)。结果抑郁障碍总患病率为440%,女性高于男性。心境恶劣、抑郁症、躯体疾病伴抑郁、适应障碍是其主要类型,患病率分别为194%、087%、079%、051%。结论抑郁障碍患病率有增高趋势。     

生活习惯及社会心理因素对广州市社区老人认知功能的影响

目的 探讨生活习惯及社会心理因素对广州市社区老人认知功能的影响.方法 采用蒙特利尔量表（MoCA）对广州市某社区≥60岁老年人进行认知功能评定,收集一般人口学及躯体疾病资料,完成生活习惯调查表、生活事件量表、社会支持评定量表等,并行相关统计分析.结果 以MoCA总分为因变量,以生活习惯、生活事件为自变量,单因素分析显示坚持运动与否与MoCA得分差异有统计学意义[（20.75±4.88）, （17.37±6.63）,P＜0.01],余组间差异无统计学意义（P＞0.05）,MoCA总分与客观社会支持、主观社会支持、支持利用度、社会支持总分均相关（r分别为0.201,0.280,0.092,0.266,均P＜0.01）;考虑性别、年龄、受教育年限、躯体疾病等因素,采用多元回归分析,进入回归方程的因素有性别、年龄、受教育年限、社会支持总分、支持利用度评分（偏回归系数分别为：-0.220,-1.530,0.313,1.654,0.503）.结论 社会支持及对社会支持的利用是老年人认知功能的保护因素,而生活习惯、生活事件对认知功能影响不大.     

美金刚口服液治疗阿尔采末病的多中心、随机、双盲、平行对照试验

目的 评价美金刚口服液治疗中、重度阿尔采末病(AD)的有效性及安全性.方法 将237例中、重度AD患者随机分为2组,试验组服用美金刚口服溶液20 mL·d-1,对照组服用美金刚片20 mg·d-1,治疗16 wk,在wk 4、8、12和16末进行AD认知评定量表(ADAS-Cog)、日常生活能力量表(ADL)和临床疗效...     

难治性抑郁症的研究进展

本文就难治性抑郁症的定义及治疗方面的研究进展进行综述。     

阿尔茨海默病及轻度认知功能障碍的生物学标志物

随着年龄的增加,MCI进展为AD的几率愈来愈大,而早期AD与其它痴呆有时又难以鉴别,所以寻找对MCI、AD早期诊断及鉴别诊断特异性高的生物学标志物成为临床研究的重点。本文就MCI、AD患者脑脊液及血液方面的生物学标志物进行综述。