自制组织芯片检测宫颈上皮内瘤变中p16蛋白的表达

目的:研究p16蛋白在宫颈上皮内瘤变(cervical intraepithelial neoplasia,CIN)中的表达及探讨其辅助诊断价值.方法:利用自制组织芯片进行184例CIN病变上皮和30例正常上皮HE光镜观察和免疫组化检测p16蛋白表达.结果:①自制组织芯片切片染色满意,达到较大标本量研究要求;②p16蛋白在10例正常对照和20例病变组织中的正常鳞状上皮与腺上皮均阴性表达,在CIN1中为88.1%(52/59),在CIN 2、CIN 3中均为100.0%表达,且表达的强度与CIN分级明显相关,有显著统计学意义(P＜0.001).CIN 1大部分阳性细胞限于上皮层的下1/3,CIN 2限于上皮的下2/3,而CIN 3则较多超过上皮的2/3或全层弥漫阳性,其表达层次与CIN分级明显相关,有显著统计学意义(P＜0.001).结论:在宫颈CIN的诊断及分级中,p16蛋白作为辅助诊断标记物有较好的应用价值;而自制的组织芯片成本低,质量上能达到科研要求,值得基层单位应用.     

丹参对兔肾热缺血时超微结构保护作用的初步观察

丹参和肌苷对实验兔肾近曲小管上皮细胞具有相似的保护作用。在热缺血60min和120min后,该处微绒毛和线粒体的缺血性损害比对照组兔为轻。但用肌苷保护的肾小管间毛细血管内皮细胞的缺血性损害比用丹参保护的要重。缺血肾恢复血流30min后,丹参与肌苷两组的微绒毛和线粒体均基本恢复正常,而对照组的这些结构未能恢复正常。丹参的主要作用之一,可能与提供维持近曲小管细胞的微绒毛有规则地排列,以及修复热缺血期间引起的局部损害所需的能量有关。     

鞘内注射JNK特异性抑制剂SP600125对CCI大鼠痛行为的影响

Objective To observe the effect of intrathecal administration of SP600125 on both MWT and TWL of rats after chronic constriction injury (CCI) of the sciatic nerve. Methods 40 male SD rats were randomized to deride into 5 groups (n=8). Rats in group SP5 received SP600125 5 μg after CCI; rats in group SP25 received SP600125 25 μg after CCI; rats in group SP50 received SP600125 50 μg after CCI; rats in group DMSO received 2% DMSO 10 μl after CCI; rats in group Naive received SP600125 50 μg without sciatic nerve injury. SP600125 was dissolved in 10 μl 2%DMSO solvent. On the 7th day after CCI, MWT and TWL were determined with yon Frey filaments and thermal radiation apparatus repectively after intrathecal administration of SP600125. Results Intrathecal administration certain dosage of SP600125 could attenuate the established mechanical allodynia and thermal hyperalgesia induced by CCI rather than normal rats. Conclusion Intrathecal administration certain dosage of SP600125 could attenuate the established mechanical allodynia and thermal hyperalgesia induced by CCI.     

鞘内注射JNK特异性抑制剂SP600125对CCI大鼠痛行为的影响

Objective To observe the effect of intrathecal administration of SP600125 on both MWT and TWL of rats after chronic constriction injury (CCI) of the sciatic nerve. Methods 40 male SD rats were randomized to deride into 5 groups (n=8). Rats in group SP5 received SP600125 5 μg after CCI; rats in group SP25 received SP600125 25 μg after CCI; rats in group SP50 received SP600125 50 μg after CCI; rats in group DMSO received 2% DMSO 10 μl after CCI; rats in group Naive received SP600125 50 μg without sciatic nerve injury. SP600125 was dissolved in 10 μl 2%DMSO solvent. On the 7th day after CCI, MWT and TWL were determined with yon Frey filaments and thermal radiation apparatus repectively after intrathecal administration of SP600125. Results Intrathecal administration certain dosage of SP600125 could attenuate the established mechanical allodynia and thermal hyperalgesia induced by CCI rather than normal rats. Conclusion Intrathecal administration certain dosage of SP600125 could attenuate the established mechanical allodynia and thermal hyperalgesia induced by CCI.     

脾炎症性肌纤维母细胞瘤肝转移1例报告并文献复习

目的：探讨炎性肌纤维母细胞瘤的临床病理特点、鉴别诊断及预后。方法：观察分析1例脾炎症性肌纤维母细胞瘤术后4 a肝转移病例的临床病理特点及免疫表型,并对相关文献复习。结果：瘤细胞梭形,呈束状、旋涡状或席纹状排列,间质大量炎症细胞弥漫浸润,脾肿瘤细胞形态温和无明显异型,肝转移灶瘤细胞轻度异型,局部呈浸润性生长;免疫组化Vimentin、SMA阳性,ALK阴性。结论：炎性肌纤维母细胞瘤是少见肿瘤,诊断须依赖病理形态及免疫组化,大多预后较好，出现异型提示预后不良。     

头痛安胶囊治疗偏头痛的临床疗效观察

目的 :研究头痛安胶囊治疗偏头痛的疗效及其机制。方法 :10 5例偏头痛患者随机分为治疗组和对照组。治疗组给予头痛安胶囊 ,对照组给予养血清脑颗粒。结果 :治疗组较对照组MCA、ACA、PCA的血流速度明显减慢 (P <0 0 5 ) ;两组血液流变学指标治疗后均有显著变化 ;治疗组头痛持续时间及发作次数明显低于对照组 (P <0 0 5 ) ;治疗组总有效率为 88 33% ,对照组总有效率为 77 78% (P <0 0 5 )。结论 :头痛安胶囊可能通过解除血管痉挛来有效控制偏头痛发作。     

痛风的饮食与禁忌

痛风,高尿酸血症的发病与体质、遗传因素有很大关系,但环境与生活方式也会产生重大影响,当痛风发作时,除治疗之外,应同时注意饮食生活的改善.     

怎样说话爸妈爱听

父母的年纪越来越大,我们做小辈的又常年不在身边,好不容易见一面,不是没话说,就是说不到一起,请问在交流方面我们应该怎样做?米女士态度上充分尊敬.面对父母时,一定要心怀敬畏,让他们感受到自己的价值和权威.可以把遇到的问题向他们请教,或者放低姿态、主动找他们商量.这样做不仅会让我们自己受益,父母也会非常有成就感.     

表没食子儿茶素没食子酸酯对人非小细胞肺癌细胞增殖的作用及机制研究

目的探讨表没食子儿茶素没食子酸酯(EGCG)对人非小细胞肺癌细胞增殖的作用及机制。方法人肺癌A549细胞随机分为三组:对照组、实验1组与实验2组,分别用含0μM、50μM、100μM浓度EGCG的DMEM培养液处理,24 h、48 h后CCK-8检测细胞增殖指数,流式细胞术检测细胞凋亡指数,48 h后流式细胞术检测细胞周期,24 h、48 h后蛋白质印迹法检测Caspase-3蛋白表达水平。结果细胞处理后24 h、48 h,实验2组与实验1组细胞增殖指数显著低于对照组(P 0. 05),实验2组也显著低于实验1组(P 0. 05)。实验2组48 h的细胞增殖指数显著高于24 h,其余两组在这两个时间点的对比无显著差异。实验2组与实验1组的细胞凋亡指数、Caspase-3蛋白相对表达水平显著高于对照组(P 0. 05),实验2组也显著高于实验1组(P 0. 05)。对照组48 h的细胞凋亡指数显著高于24 h,其余各组的细胞凋亡指数及三组的Caspase-3表达在这两个时间点的对比无显著差异。细胞处理后48 h,实验2组与实验1组的G1期细胞比率显著高于对照组(P 0. 05),G2期细胞比率显著低于对照组(P 0. 05)。结论表没食子儿茶素没食子酸酯呈剂量依赖式抑制人非小细胞肺癌细胞的增殖,促进其凋亡,调节其细胞周期,促进Caspase-3的表达,可发挥潜在的抑瘤作用。