全文获取类型
收费全文 | 903篇 |
免费 | 94篇 |
国内免费 | 6篇 |
专业分类
耳鼻咽喉 | 13篇 |
儿科学 | 23篇 |
妇产科学 | 10篇 |
基础医学 | 193篇 |
口腔科学 | 60篇 |
临床医学 | 76篇 |
内科学 | 245篇 |
皮肤病学 | 12篇 |
神经病学 | 63篇 |
特种医学 | 39篇 |
外科学 | 74篇 |
综合类 | 29篇 |
预防医学 | 66篇 |
眼科学 | 26篇 |
药学 | 54篇 |
中国医学 | 3篇 |
肿瘤学 | 17篇 |
出版年
2023年 | 7篇 |
2022年 | 19篇 |
2021年 | 41篇 |
2020年 | 16篇 |
2019年 | 24篇 |
2018年 | 31篇 |
2017年 | 23篇 |
2016年 | 26篇 |
2015年 | 22篇 |
2014年 | 41篇 |
2013年 | 46篇 |
2012年 | 47篇 |
2011年 | 64篇 |
2010年 | 51篇 |
2009年 | 39篇 |
2008年 | 34篇 |
2007年 | 53篇 |
2006年 | 58篇 |
2005年 | 55篇 |
2004年 | 26篇 |
2003年 | 18篇 |
2002年 | 19篇 |
2001年 | 20篇 |
2000年 | 19篇 |
1999年 | 19篇 |
1998年 | 23篇 |
1997年 | 18篇 |
1996年 | 14篇 |
1995年 | 10篇 |
1994年 | 13篇 |
1993年 | 10篇 |
1992年 | 12篇 |
1991年 | 6篇 |
1990年 | 7篇 |
1989年 | 3篇 |
1988年 | 8篇 |
1987年 | 6篇 |
1986年 | 9篇 |
1985年 | 3篇 |
1984年 | 3篇 |
1983年 | 6篇 |
1981年 | 6篇 |
1975年 | 5篇 |
1974年 | 1篇 |
1971年 | 4篇 |
1970年 | 1篇 |
1969年 | 2篇 |
1968年 | 5篇 |
1967年 | 3篇 |
1966年 | 1篇 |
排序方式: 共有1003条查询结果,搜索用时 46 毫秒
1.
J A L Barbosa J Maciel A C Vale A C Saraiva 《European journal of surgical oncology》2005,31(4):406-409
OBJECTIVES: Assessment of morphological characteristics of lymphnodes studied ex-corpore by echoendoscopy--shape, sharpness of limits, echogenicity and dimensions--to categorize them either as metastatic or non-metastatic. MATERIAL AND METHODS: Fifty-four lymphnodes were studied. They were identified and studied by echoendoscopy in fresh surgical specimens of oesophagus, stomach and rectum. Eight nodal characteristics were evaluated. The data obtained were studied by multivariate analysis using the logistical estimate method in two different statistical models. RESULTS: In model 1, logistical estimate demonstrated that well-defined limits (WDL) of the lymphnodes and the association of hypoechogenicity and round shape were the most significant variables suggesting the presence of invasion. In model 2, the most significant variables were WDL, round shape and association of round shape with WDL. The first model had a high sensitivity of 93.1% and specificity of 68% whereas the second model had a greater specificity of 84%, with a slight fall in sensitivity. CONCLUSION: These models might have an application to clinical practice particularly in the pre-operative assessment of patients with oesophageal carcinoma, gastric carcinoma or rectal carcinoma. 相似文献
2.
高效液相色谱法测定右旋儿茶素血浆浓度及药代动力学参数 总被引:1,自引:0,他引:1
本文建立了体液中右旋儿茶素的RP-HPLC测定方法。采用C_(18)键合相硅胶为填料的固相提取柱进行样品预处理,右旋儿茶素的提取回收率为79.8%.应用二极管阵列检测器对色谱峰纯度进行鉴定。该法精密度好,方法回收率近100%,日内、日间的变异系数为2.4~5.6%,血浓69.6~1160 ng/ml范围内呈线性关系,r=0.9993。家兔静注右旋儿茶素18mg/kg,其药代动力学过程符合二室模型,分布相半衰期为0.129 h,消除相半衰期为1.19h。 相似文献
3.
4.
Pamella Huey Mei Wang Marcos Antonio Cenedeze Joo Bosco Pesquero Alvaro Pacheco-Silva Niels Olsen Saraiva Cmara 《International immunopharmacology》2006,6(13-14):1960
Bradykinin B1 receptors are exclusively expressed in inflamed tissues. For this reason, they have been related with the outcomes of several pathologies. Ischemia–reperfusion injury is caused by the activation of inflammatory and cytoprotective genes, such as macrophage chemoattractant protein-1 and heme oxygenase-1, respectively. This study was aimed to analyze the involvement of bradykinin B1 and B2 receptors (B1R and B2R) in tissue response after renal ischemia–reperfusion injury. For that, B1R (B1−/−), B2R (B2−/−) knockout animals and its control (wild-type mice, B1B2+/+) were subjected to renal bilateral ischemia, followed by 24, 48 and 120 h of reperfusion. At these time points, blood serum samples were collected for creatinine and urea dosages. Kidneys were harvested for histology and molecular analyses by real-time PCR. At 24 and 48 h of reperfusion, B1−/− group resulted in the lowest serum creatinine and urea levels, indicating less renal damage, which was proved by renal histology. Renal protection associated with B1−/− mice was also related with higher expression of HO-1 and lower expression of MCP-1. In conclusion, the absence of B1R had a protective role against inflammatory responses developed after renal ischemia–reperfusion injury. 相似文献
5.
6.
7.
Sousa MM Ferrão J Fernandes R Guimarães A Geraldes JB Perdigoto R Tomé L Mota O Negrão L Furtado AL Saraiva MJ 《Laboratory investigation; a journal of technical methods and pathology》2004,84(7):865-873
Familial amyloid polyneuropathy (FAP) is characterized by deposition of mutated transthyretin (TTR) in the peripheral nervous system. Prior to amyloid fibrils, nonfibrillar TTR aggregates are deposited inducing oxidative stress with increased nitration (3-NT). As the major source of TTR is the liver, liver transplantation (LT) is used to halt FAP. Given the shortage of liver donors, domino LT (DLT) using FAP livers is performed. The correlation between TTR deposition in the skin and nerve was tested in biopsies from normal individuals, asymptomatic carriers (FAP 0) and FAP patients; in FAP 0, nonfibrillar TTR was observed both in the skin and nerve in the same individuals; in patients, amyloid was detected in both tissues. The occurrence of amyloidosis in recipients of FAP livers was evaluated 1-7 years after DLT: TTR deposition occurred in the skin 3 years after transplantation either as amyloid or aggregates; in one of the recipients, fibrillar TTR was present in the epineurium 6 years after DLT. Deposits were scarce and 3-NT immunostaining was irrelevant. Nerve biopsies from DLT recipients had no FAP-related neuropathy. Our findings suggest that TTR amyloid formation occurs faster than predicted and that TTR of liver origin can cross the blood-nerve barrier. Recipients of FAP livers should be under surveillance for TTR deposition and tissue damage. 相似文献
8.
The UTX gene escapes X inactivation in mice and humans 总被引:7,自引:3,他引:7
Greenfield A; Carrel L; Pennisi D; Philippe C; Quaderi N; Siggers P; Steiner K; Tam PP; Monaco AP; Willard HF; Koopman P 《Human molecular genetics》1998,7(4):737-742
We recently have identified a ubiquitously transcribed mouse Y chromosome
gene, Uty , which encodes a tetratricopeptide repeat (TPR) protein. A
peptide derived from the UTY protein confers H-Y antigenicity on male
cells. Here we report the characterization of a widely transcribed X-linked
homologue of Uty , called Utx , which maps to the proximal region of the
mouse X chromosome and which detects a human X-linked homologue at Xp11.2.
Given that Uty is ubiquitously transcribed, we assayed for Utx expression
from the inactive X chromosome (Xi) in mice and found that Utx escapes X
chromosome inactivation. Only Smcx and the pseudoautosomal Sts gene on the
mouse X chromosome have been reported previously to escape inactivation.
The human UTX gene was also found to be expressed from Xi. We discuss the
significance of these data for our understanding of dosage compensation of
X-Y homologous genes in humans and mice.
相似文献
9.
10.
Common origin of the Val30Met mutation responsible for the amyloidogenic transthyretin type of familial amyloidotic polyneuropathy 总被引:3,自引:0,他引:3