MiR-200c-3p inhibits cell migration and invasion of clear cell renal cell carcinoma via regulating SLC6A1

In this study, we investigated the mechanism of miR-200c-3p and SLC6A1 in regulating cell activity of clear cell renal cell carcinoma (CCRCC). The mRNA and miRNA expressions of tissue specimens were analyzed by CapitalBio Corporation (Beijing, China). The expression of SLC6A1 in CCRCC cells was examined through qRT-PCR and western blot. The migration and invasion ability of 786-O cells was testified by transwell assay after transfected. 786-O cell proliferation ability was detected by MTT assay. Dual luciferase reporter assay verified the association between SLC6A1 and miR-200c-3p. SLC6A1 was high expressed and miR-200c-3p was low expressed in CCRCC tissues and cells. Besides, lower SLC6A1 expression indicated longer survival time and higher survival rate. MiR-200c-3p could directly target at SLC6A1 and reduce its expression. MiR-200c-3p inhibited the proliferation, migration and invasion in 786-O cells by down-regulating SLC6A1 expression. The results suggested that the miR-200c-3p served as a suppressor for CCRCC via down-regulating SLC6A1.     

Unusual electrophysiological findings in a Chinese ALS 4 family with SETX-L389S mutation: a three-year follow-up

Journal of Neurology - Amyotrophic lateral sclerosis type 4 (ALS4) is a familial form of ALS caused by mutations in the SETX gene. To date, there are seven unrelated ALS4 families with four...     

产后6个月内乳母营养摄入与哺乳的跟踪观察

对北京市城乡50名产后哺乳妇女6个月内营养素摄入量和乳汁分泌以及乳成分进行跟踪观察。结果表明:乳母的营养素摄入量在产后第1个月内除了钙以外均超过我国膳食营养素供给量(RDA),但第3和第6个月时都下降,尤以钙、维生素A及B_2明显。母乳的分泌量在产后前3个月,城乡分别为689g及803g,至6个月时下降,以城区的下降更明显,但都能接近或超过发达国家乳母的母乳分泌量。城区母乳中热能、蛋白质、脂肪和乳糖的含量均高于农村的,可能与城市乳母膳食质量较高有关。从母乳分泌量及其营养成分看,母乳喂养婴儿到3个月能满足婴儿营养的需要,而农村还可以维持到6个月。     

喉组织癌前病变的DNA流式定量研究

应用流式细胞术对80例喉癌前病变细胞中的DNA含量进行定量检测.结果显示,29例I级不典型增生中出现DNA异倍体1例,30例Ⅱ级不典型增生中出现DNA异倍体2例,21例Ⅲ级不典型增生中出现DNA异倍体11例.提示喉癌前病变的DNA异倍体率随不典型增生病变的分级增高而增加,不典型增生程度越高,癌变率越高,提示DNA异倍体可能是喉癌变早期的分子标志物.     

米非司酮联合氨甲蝶呤及微波治疗异位妊娠的疗效分析

目的:观察米非司酮联合氨甲蝶呤及微波治疗异位妊娠的临床效果。方法:将82例异位妊娠患者随机分为两组,观察组41例采用米非司酮联合氨甲蝶呤及微波治疗,对照组41例仅采用米非司酮联合氨甲蝶呤进行治疗,比较两组的治疗成功率及治疗前后β-HCG水平和包块直径变化。结果:观察组的治疗成功率明显高于对照组(P<0.05)。两组治疗后每周包块直径逐渐下降,并且观察组治疗后β-HCG水平和包块直径均明显低于同期对照组,两组比较差异有统计学意义(P<0.05)。结论:米非司酮联合氨甲蝶呤及微波对异位妊娠进行治疗,能够明显提高临床疗效,显著降低β-HCG水平和减小包块直径,值得临床推广应用。     

新疆农牧区卫生事业在改革中快速发展

新疆维吾尔自治区现有人口 184 6万 ,面积 160万平方公里 ,是一个以维吾尔为主体民族 ,多民族聚居的边远少数民族省区 ,农牧业人口占 65 .4 5 %。因此 ,大力加强和发展农牧区卫生事业一直是自治区卫生改革工作的重点。 1996年中央召开全国卫生工作大会以来 ,按照《中共中央、国务院关于卫生改革与发展的决定》精神 ,自治区党委和人民政府对卫生改革特别是农牧区卫生事业的改革与发展给予了高度重视。在各级党政和有关部门的重视与支持下 ,经过卫生等部门的共同努力 ,农牧区卫生事业取得了长足的进步和快速发展。1　推进农牧区卫生工作的做法…     

托幼儿童身长体重及营养素摄入水平的研究:四年系统观察

1979～1983连续四年观察了两个全托托儿所和一个全托幼儿园儿童的身长、体重及膳食营养状况。结果表明,到1982年时,儿童身长、体重在标准均值以上的人数均超过50％,托儿所儿童能量及蛋白质的摄入量加上零食中的含量能达到供给量标准。各类食物摄入量也能达到或超过北京市推荐的托儿所儿童参考食物量标准。幼儿园儿童营养素的摄入量与供给量比较都偏低,但其生长发育良好,故对该年龄组儿童的营养素供给量应再进行研究。     

Ketamine Induces Toxicity in Human Neurons Differentiated from Embryonic Stem Cells via Mitochondrial Apoptosis Pathway

Ketamine is widely used for anesthesia in pediatric patients. Growing evidence indicates that ketamine causes neurotoxicity in a variety of developing animal models. Our understanding of anesthesia neurotoxicity in humans is currently limited by difficulties in obtaining neurons and performing developmental toxicity studies in fetal and pediatric populations. It may be possible to overcome these challenges by obtaining neurons from human embryonic stem cells (hESCs) in vitro. hESCs are able to replicate indefinitely and differentiate into every cell type. In this study, we investigated the toxic effect of ketamine on neurons differentiated from hESCs. Two-week-old neurons were treated with different doses and durations of ketamine with or without the reactive oxygen species (ROS) scavenger, Trolox. Cell viability, ultrastructure, mitochondrial membrane potential (ΔΨm), cytochrome c distribution within cells, apoptosis, and ROS production were evaluated. Here we show that ketamine induced ultrastructural abnormalities and dose- and timedependently caused cell death. In addition, ketamine decreased ΔΨm and increased cytochrome c release from mitochondria. Ketamine also increased ROS production and induced differential expression of oxidative stress-related genes. Specifically, abnormal ultrastructural and ΔΨm changes occurred earlier than cell death in the ketamine-induced toxicity process. Furthermore, Trolox significantly decreased ROS generation and attenuated cell death caused by ketamine in a dose-dependent manner. In conclusion, this study illustrates that ketamine time- and dose-dependently induces human neurotoxicity at supraclinical concentrations via ROS-mediated mitochondrial apoptosis pathway and that these side effects can be prevented by the antioxidant agent Trolox. Thus, hESC-derived neurons might provide a promising tool for studying anesthetic-induced developmental neurotoxicity and prevention strategies.     

细胞间黏附分子-1及血管细胞黏附分子-1与突发性耳聋

突发性耳聋的发病机制目前尚未阐明.近年来,国外学者报道突发性耳聋患者血浆中细胞间黏附分子-1(ICAM-1)和血管细胞黏附分子-1(VCAM-1)水平增高.现将有关突发性耳聋与ICAM-1和VCAM-1关系作一综述,为突发性耳聋的发病机制及治疗提供新的思路.     

丙泊酚复合瑞芬太尼全凭静脉或地氟醚平衡麻醉对听神经瘤手术后苏醒的影响

目的 观察丙泊酚复合瑞芬太尼全凭静脉或地氟醚平衡麻醉对听神经瘤手术后苏醒的影响。方法 40例ASAⅠ~Ⅱ级择期行乙状窦入路或颅中窝入路听神经瘤手术的成年患者,随机分为丙泊酚复合瑞芬太尼组(PR组,n=20) 和地氟醚平衡麻醉组(DR组,n=20)。记录患者一般情况;记录唤醒时间、拔除气管导管时间和定向力恢复时间;记录2组患者手术结束时的麻醉深度、肌肉松弛恢复情况、拔管时心率和血压变化;记录术后不良事件。结果 PR组唤醒时间早于DR组2.4 min (P=0.033);PR组拔除气管导管时间短于DR组4.9 min (P=0.002);PR组定向力恢复时间短于DR组23.7 min(P=0.049);PR组拔除气管导管时血压和心率变化均小于DR组(P<0.05)。结论 丙泊酚复合瑞芬太尼全凭静脉麻醉用于听神经瘤手术利于患者术后快速苏醒,地氟醚组患者拔管时心率和血压变化较明显。