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1.
Comparison of spot esculin hydrolysis with the PathoTec strip test for rapid differentiation of anaerobic bacteria.
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S M Qadri S Johnson J C Smith S Zubairi R L Gillum 《Journal of clinical microbiology》1981,13(3):459-462
The ability of several anaerobic bacteria to hydrolyze esculin to esculetin is used by clinical microbiologists and taxonomists in the differentiation and identification of both gram-positive and gram-negative microorganisms. Conventional methods used for determining esculin hydrolysis by anaerobic bacteria require 24 to 48 h for completion. In this paper we evaluate two procedures which yield rapid results. A total of 738 anaerobic bacteria were used in this study. A total of 99% of the esculin-hydrolyzing anaerobic bacteria gave positive results with the spot test in 1 h, whereas the other test method, the PathoTec strip test (General Diagnostics, Morris Plains, N.J.), required 4 h for 96% of the strains tested to yield positive reactions. Both tests showed a 100% specificity when compared with the standard broth test and are easy to perform, accurate, and economical. The spot test is superior to the PathoTec strip test in yielding results more rapidly. 相似文献
2.
Akbar Jaleel Zubairi Haroon Rashi Rizwan Haroon Rashi Moiz Ali Pervaiz Mehmood Hashmi 《中华创伤杂志(英文版)》2017,20(3):147-150
Objective: To evaluate the effectiveness of Judet''s quadricepsplasty for treatment of knee contractures and to identify the effect of local infiltration of epinephrine on blood loss associated with this procedure.
Methods: A retrospective cohort study was conducted in which all cases of knee contractures managed
with Judet''s quadricepsplasty from 1st January 2009 to 31st December 2013 were included and were
divided into two groups. The epinephrine group included patients who were infiltrated with diluted
epinephrine (1:400,000) along with xylocaine, around the operative field 15 min prior to the incision
time, while the control group did not receive any infiltration. Judet''s outcome, blood loss, drop in hemoglobin and required blood transfusion were noted for all patients and compared between both groups.
Results: Most common preceding pathology identified for the development of knee contractures was
periarticular fracture while ilizarov application was the most common etiology. Both groups were found similar in all preoperative characteristics except preoperative flexion contracture (p=0.02). All functional outcome measures including Judet''s outcome were similar in both groups. In contrast, duration of surgery (p=0.01), blood loss (p=0.02), drop in hemoglobin (p=0.01) and number of transfusions (p=0.03) were significantly reduced in epinephrine group.
Conclusion: Judet''s quadricepsplasty is a useful procedure to increase the range of motion of rigid knees and local infiltration of epinephrine is effective in decreasing the amount of subsequent blood loss and transfusion requirements. 相似文献
3.
Basit A Zubairi A Rehman AZ 《Journal of the College of Physicians and Surgeons--Pakistan : JCPSP》2004,14(6):374-375
A case report of lytic lesion of the first metacarpal bone is presented, which was a conventional grade III giant cell tumour. It was excised and reconstructed by a free fibular graft with gratifying result after three years follow-up. 相似文献
4.
Ashraf K Raza SS Ashraf O Memon W Memon A Zubairi TA 《The British journal of radiology》2007,80(954):e117-e118
Renal lymphangiectasia is a benign disorder of renal lymphatics. Seldom observed, the pathophysiology is unclear. The clinical course may vary, and management alternatives range from percutaneous drainage in symptomatic cases to pharmacological substitutes in the form of anti-hypertensives and diuretics. We present a case of bilateral perinephric collections on imaging, which presented with gross ascites, abdominal pain and reversible hypertension. Ultrasound examination indicated ascites. Computerized tomography revealed bilateral symmetrical large perinephric collections. This is consistent with the appearance of renal lymphangiectasis (enlarged kidneys with fluid collections seen to be abutting the surrounding structures) reported in the literature. Needle aspiration of the perinephric fluid was undertaken, and laboratory analysis was carried out, which revealed a protein level of 643 mg dl(-1) and a total leucocyte count of 50, of which 80% were lymphocytes. Thereafter, a diagnosis of renal lymphangiectasia was made, and conservative treatment with diuretics and anti-hypertensives was initiated. Reassessment at subsequent follow up visits showed improvement in the patient's clinical condition. 相似文献
5.
6.
H Nawaz S Z Imam A B S Zubairi A H Pabaney Y J Sepah M Islam J A Khan 《The international journal of tuberculosis and lung disease》2007,11(8):915-919
OBJECTIVE: To assess the habits, knowledge and attitudes towards smoking among Pakistani medical students. METHODS: A cross-sectional study was conducted from March 2004 to July 2005 at three medical colleges using a World Health Organization (WHO) questionnaire. A total of 1,029 medical students participated in the study. RESULTS: The prevalence of smoking was found to be 11.2%. Smoking was more prevalent among males, hostel residents and first year medical students. Almost half of the smokers had tried to quit smoking. A family member or a friend was considered to be the most likely person to help quit smoking. The majority believed that passive smoking was harmful to health and were generally supportive of legislative measures to reduce tobacco use, such as the restriction of smoking in public places and the prohibition of sale of tobacco to children. Lesser but significant numbers thought that there should be a complete ban on smoking advertisements and that the price of tobacco products should be increased. CONCLUSION: The study shows a high prevalence of tobacco use in future physicians in spite of adequate knowledge about and a satisfactory attitude towards smoking. 相似文献
7.
SahBandar IN Takahashi K Motomura K Djoerban Z Firmansyah I Kitamura K Sato H Pohan HT Sato S 《AIDS research and human retroviruses》2011,27(1):97-102
Cocirculation of subtype B and CRF01_AE in Southeast Asia has led to the establishment of new recombinant forms. In our previous study, we found five samples suspected of being recombinants between subtype B and CRF01_AE, and here, we analyzed near full-length sequences of two samples and compared them to known CRFs_01B, subtype B, and CRF01_AE. Five overlapped segments were amplified with nested PCR from PBMC DNA, sequenced, and analyzed for genome mosaicism. The two Indonesian samples, 07IDJKT189 and 07IDJKT194, showed genome-mosaic patterns similar to CRF33_01B references from Malaysia, with one short segment in the 3' end of the p31 integrase-coding region, which was rather more similar to subtype B than CRF01_AE, consisting of unclassified sequences. These results suggest gene-specific continuous diversification and spread of the CRF33_01B genomes in Southeast Asia. 相似文献
8.
Adoptive immunotherapy against experimental visceral leishmaniasis with CD8+ T cells requires the presence of cognate antigen
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Polley R Stager S Prickett S Maroof A Zubairi S Smith DF Kaye PM 《Infection and immunity》2006,74(1):773-776
CD8+ T cells have a protective role in experimental visceral leishmaniasis. However, the observation that inflammatory cytokines induce bystander activation of CD8+ T cells questions the need for antigen-dependent effector function. Here, we demonstrate that successful adoptive immunotherapy with CD8+ T cells is strictly dependent upon the presence of cognate antigen. 相似文献
9.
Muhammad Arsalan Raffat Naila Irum Hadi Mervyn Hosein Adnan Mustafa Zubairi Sana Ikram Zohaib Akram 《Saudi Dental Journal》2019,31(1)
Aim
To investigate the expression of salivary S100A7 levels among patients with oral submucous fibrosis (OSF) and healthy controls.Method
A total number of 60 participants were included in the study (30 OSF cases and 30 healthy controls). Demographic data was collected using a structured baseline questionnaire. Salivary S100A7 levels were quantified using enzyme-linked immunosorbent assay. Data was analyzed using Student t-test. Pearson correlation test was used to evaluate correlation between S100A7 levels and independent variables such as frequency and duration of areca nut use, gutka use, and mouth opening.Results
The mean value of salivary S100A7 for OSF group was 0.275?ng/ml, whereas mean value of salivary S100A7 for healthy controls was 0.195?ng/ml. Student t-test indicated that there was statistically significantly higher levels of S100A7 in OSF group as compared to healthy controls (p?<?.001). When the clinical variables of individual groups were analysed, a significant negative correlation was found between salivary S100A7 and duration of areca nut (p?=?.009) and gutka chewing (p?=?.03), whereas a significant positive correlation was found for mouth opening (p?=?.04).Conclusion
OSF presented higher levels of salivary S100A7 levels as compared with healthy individuals and may be used as surrogate measure to identify subjects at risk for OSF. 相似文献10.
Bronchiolitis obliterans organising pneumonia is a clinicopathological syndrome characterised by indolent course of flu-like illness followed by cough, dyspnoea and fever, and responds well to steroid treatment. We describe a 76-year-old woman who presented with a short three-day history and progressed rapidly to respiratory failure. She was successfully treated with high-dose steroids. 相似文献