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排序方式: 共有121条查询结果,搜索用时 10 毫秒
1.
J. S. Sarkissian A. A. Galoyan R. G. Kamalyan V. A. Chavushyan I. B. Meliksetyan M. V. Poghosyan O. V. Gevorkyan A. S. Hovsepyan Z. E. Avakyan S. A. Kazaryan M. K. Manucharyan 《Neurochemical Journal》2007,1(3):227-234
The changes in spike activity of single neurons of the compact part of the substantia nigra, evoked by nucleus caudatus stimulation under conditions of long-term registration of the single and multiple, isolated and combined actions of GABA, GABA-amide, glutamine, and ethanolamine-O-sulfate (EOS) were studied in albino rats. Inhibition of poststimulus activity under GABA action was recorded and the inhibitory effect of GABA-amide was revealed. Primary excitatory and subsequent inhibitory effects of glutamine in combination with EOS were shown. The subsequent administration of bacterial melanin, synthesized by a mutant culture of Bacillus thuringiensis (BT-M) evoked a clear-cut and prolonged excitatory reaction during all the combined actions of GABA, GABA-amide, glutamine, and EOS. Preliminary administration of BT-M abolished the inhibitory poststimulus effects of GABA, GABA-amide, and EOS, as well as glutamine-induced excitation. 相似文献
2.
Progesterone stimulation of Xenopus oocyte maturation requires the cytoplasmic polyadenylation-induced translation of mos and cyclin B mRNAs. One cis element that drives polyadenylation is the CPE, which is bound by the protein CPEB. Polyadenylation is stimulated by Aurora A (Eg2)-catalyzed CPEB serine 174 phosphorylation, which occurs soon after oocytes are exposed to progesterone. Here, we show that insulin also stimulates Aurora A-catalyzed CPEB S174 phosphorylation, cytoplasmic polyadenylation, translation, and oocyte maturation. However, these insulin-induced events are uniquely controlled by PI3 kinase and PKC-zeta, which act upstream of Aurora A. The intersection of the progesterone and insulin signaling pathways occurs at glycogen synthase kinase 3 (GSK-3), which regulates the activity of Aurora A. GSK-3 and Aurora A interact in vivo, and overexpressed GSK-3 inhibits Aurora A-catalyzed CPEB phosphorylation. In vitro, GSK-3 phosphorylates Aurora A on S290/291, the result of which is an autophosphorylation of serine 349. GSK-3 phosphorylated Aurora A, or Aurora A proteins with S290/291D or S349D mutations, have reduced or no capacity to phosphorylate CPEB. Conversely, Aurora A proteins with S290/291A or S349A mutations are constitutively active. These results suggest that the progesterone and insulin stimulate maturation by inhibiting GSK-3, which allows Aurora A activation and CPEB-mediated translation. 相似文献
3.
Regulated CPEB phosphorylation during meiotic progression suggests a mechanism for temporal control of maternal mRNA translation
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CPEB is an mRNA-binding protein that stimulates polyadenylation-induced translation of maternal mRNA once it is phosphorylated on Ser 174 or Thr 171 (species-dependent). Disruption of the CPEB gene in mice causes an arrest of oogenesis at embryonic day 16.5 (E16.5), when most oocytes are in pachytene of prophase I. Here, we show that CPEB undergoes Thr 171 phosphorylation at E16.5, but dephosphorylation at the E18.5, when most oocytes are entering diplotene. Although phosphorylation is mediated by the kinase aurora, the dephosphorylation is due to the phosphatase PP1. The temporal control of CPEB phosphorylation suggests a mechanism in which CPE-containing mRNA translation is stimulated at pachytene and metaphase I. 相似文献
4.
DIFFERENCES IN FREE AMINO ACID CONTENT OF SEEDLINGS OF AWNED AND HOODED BARLEY, AND THEIR ALTERATION BY CHLORAMPHENICOL TREATMENT 总被引:1,自引:0,他引:1
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Blockade of angiotensin type 1 (AT1) receptors induces smooth muscle cell (SMC) death and regression of aortic hypertrophy in spontaneously hypertensive rats (SHR). We postulated that SMC death and vascular remodeling in this model may be attenuated by z-Val-Ala-Asp(OMe)-CH2F (z-VAD-fmk), a tripeptide inhibitor of caspase enzymes mediating apoptosis. To determine the time course of SMC death and aortic remodeling, SHR were treated with losartan (30 mg/kg per day) for up to 9.5 days. Transient SMC apoptosis occurred in the aortic media with a peak around day 5 of treatment, with increases in the Bax to Bcl-2 protein ratio (>3-fold), in active caspase-3 (5.6-fold), in TUNEL-positive nuclei (19-fold), preceding by 24 hours the peak activation of capase-9 (3.8-fold), and significant reductions in SMC number (46%) and aortic cross-sectional area (8.5%) at 5.5 days. The decrease in total aortic DNA reached significance at 6.5 days (29%). Blood pressure reduction with losartan was progressive and reached significance at day 7 of treatment. Next, we examined the causal link between vascular apoptosis and remodeling. SHR received placebo or losartan (30 mg/kg per day) for 6 days. During the last 24 hours, a subgroup of losartan-treated rats received 3 IV injections of z-VAD-fmk (cumulative dose: 4.4 mg x kg(-1)). All other rats received the vehicle, DMSO. The 24-hour cotreatment with z-VAD-fmk effectively prevented losartan-induced caspase-3 activation and internucleosomal DNA fragmentation, as well as SMC depletion and the reductions in aortic mass and DNA content. Together, these data suggest that caspase-dependent SMC death mediates the early phase of vascular remodeling in response to AT1 receptor blockade in this model of hypertension. 相似文献
7.
A. L. Minasyan A. V. Aznauryan I. B. Meliksetyan V. A. Chavushyan J. S. Sarkissian 《Neurochemical Journal》2011,5(1):52-68
We performed morphological, histochemical, and electrophysiological analysis of changes in the state of the rat sciatic nerve
after nerve crushing. The activity of Ca2+-dependent acidic phosphatase (AP) was measured in sections of the nerve fibers to trace the recovery of the nerve after its
injury. The systemic application of parathyroid hormone (PTH) resulted in the acceleration of injured nerve recovery. Proliferation
of both endoneurium and Schwann cells was observed on the 3rd day after the crushing of the ipsilateral extensor (N. peroneus communis) and flexor (N. gastrocnemius) nerve bundles. A complete recovery of the AP enzymatic activity was found on the 7–9th days after the crushing. From the
5th to 35th days after the crushing, we performed online registration and mathematical analysis of pulse activity. In the
control experiments, from the 35th to 70th days, high-frequency stimulation of the nerves showed the regeneration of the extensor
nerve only accompanied by alterations in fiber direction. In the presence of PTH, the regeneration was significantly accelerated
and it was associated with early regeneration of the flexor nerve and more structured fiber morphology. We found a correlation
between the electrophysiological and morphological data. 相似文献
8.
A. L. Minasyan A. V. Aznauryan I. B. Meliksetyan V. A. Chavushyan J. S. Sarkissian A. A. Galoyan 《Neurochemical Journal》2011,5(4):278-284
A comparative morphological and histochemical study of the dynamics of de- and regenerative processes in the damaged sciatic
nerve (SN) area was conducted in rats at different time points after crushing in the presence of proline-rich peptide (PRP-1).
We measured the activity of Ca2+-dependent acid phosphatase (AP). At the fifth day after SN crushing on the site of injury, we observed changes in the directions
of nerve fibers and disruption of their structure, in particular, swelling of Schwann hollow tubes. Later, we found proliferation
of cells of the endoneurium and Schwann cells in the extensor bundle (N. peroneus communis), which promoted recovery of nerve fibers and was accompanied by appearance of the maximum activity AP. We also showed partial
recovery of the flexor bundle (N. Gastrocnemius). At 17 days after the trauma, as a result of successful regeneration of the damaged nerve, we observed the recovery of nerve
fibers and their structures, which confirms the neuroprotective characteristics of PRP-1 相似文献
9.
Renal ischemia-reperfusion injury in the rat is prevented by a novel immune modulation therapy 总被引:3,自引:0,他引:3
Tremblay J Chen H Peng J Kunes J Vu MD Der Sarkissian S deBlois D Bolton AE Gaboury L Marshansky V Gouadon E Hamet P 《Transplantation》2002,74(10):1425-1433
BACKGROUND: Vasogen Inc.'s (Mississauga, Ontario, Canada) immune modulation therapy (IMT) is a therapy in which cells from the patient's own blood are modified by ex vivo exposure to specific physicochemical stressors, including oxidation, ultraviolet (UV) light, and an elevated temperature. The therapy has been shown to have a beneficial effect in models of inflammation and vascular diseases. This study tested the hypothesis that IMT can prevent renal ischemia-reperfusion (I/R) injury in rats. METHODS: Whole blood was collected from syngeneic age-matched donors by cardiac puncture. It was treated with a combination of controlled physiochemical stressors consisting of elevated temperature, a gas mixture of medical oxygen containing ozone, and UV light. The treated blood (150 microL) was injected in the gluteal muscle. Control animals received the same volume of untreated blood or physiological saline. Transient (45 or 60 minutes) left-renal ischemia was produced with simultaneous contralateral nephrectomy in treated and control spontaneously hypertensive rats (SHR). Young and old male and female rats were studied. Plasma creatinine, diuresis, and the survival rates of each group were compared. Renal apoptosis-necrosis was estimated by DNA laddering, histology, and in situ terminal deoxynucleotidyl transferase assay. mRNA levels of several regulators of apoptosis-regeneration were determined in control and postischemic kidneys by Northern blotting. RESULTS: IMT pretreatment of SHR significantly reduced renal I/R injury compared with equivalent placebo treatments consisting of untreated blood- or saline-injected SHR, as evidenced by a significant increase of the survival rate curves in young and old male SHR, which correlated with 24-hour postischemic diuresis. The increases in plasma creatinine following renal I/R were significantly lower in IMT-treated young male and old female SHR compared with saline or untreated blood-injected controls. Dilution analysis showed that the protective effect of treated blood was lost by dilution. Loss of epithelial cells was reduced in IMT-treated rats, with a significant decline in the peak of apoptosis 12 hours after acute ischemic renal injury. IMT did not modify the pattern of mRNA levels of several genes involved in the inflammation and regeneration processes. CONCLUSION: Our data demonstrate that IMT prevents the destruction of kidney tissue and the resulting animal death caused by renal I/R injury. 相似文献
10.