全文获取类型
收费全文 | 1020篇 |
免费 | 99篇 |
专业分类
耳鼻咽喉 | 15篇 |
儿科学 | 32篇 |
妇产科学 | 37篇 |
基础医学 | 201篇 |
口腔科学 | 4篇 |
临床医学 | 66篇 |
内科学 | 123篇 |
皮肤病学 | 6篇 |
神经病学 | 148篇 |
特种医学 | 68篇 |
外科学 | 124篇 |
综合类 | 10篇 |
预防医学 | 82篇 |
眼科学 | 50篇 |
药学 | 34篇 |
中国医学 | 1篇 |
肿瘤学 | 118篇 |
出版年
2023年 | 9篇 |
2022年 | 7篇 |
2021年 | 18篇 |
2020年 | 10篇 |
2019年 | 26篇 |
2018年 | 38篇 |
2017年 | 17篇 |
2016年 | 20篇 |
2015年 | 15篇 |
2014年 | 34篇 |
2013年 | 49篇 |
2012年 | 73篇 |
2011年 | 79篇 |
2010年 | 34篇 |
2009年 | 32篇 |
2008年 | 57篇 |
2007年 | 73篇 |
2006年 | 55篇 |
2005年 | 54篇 |
2004年 | 37篇 |
2003年 | 28篇 |
2002年 | 33篇 |
2001年 | 15篇 |
2000年 | 17篇 |
1999年 | 17篇 |
1998年 | 12篇 |
1997年 | 6篇 |
1995年 | 9篇 |
1994年 | 7篇 |
1993年 | 12篇 |
1992年 | 17篇 |
1991年 | 9篇 |
1990年 | 6篇 |
1989年 | 9篇 |
1988年 | 23篇 |
1987年 | 21篇 |
1985年 | 10篇 |
1984年 | 6篇 |
1983年 | 5篇 |
1982年 | 5篇 |
1981年 | 5篇 |
1980年 | 7篇 |
1979年 | 11篇 |
1978年 | 7篇 |
1977年 | 6篇 |
1974年 | 10篇 |
1972年 | 11篇 |
1971年 | 9篇 |
1970年 | 6篇 |
1966年 | 5篇 |
排序方式: 共有1119条查询结果,搜索用时 250 毫秒
1.
Iver A. Langmoen Tryggve Lundar Ingebjørg Storm-Mathisen Sverre O. Lie Karl H. Hovind 《Child's nervous system》1991,7(1):13-15
We present 36 consecutive patients with intrinsic glioma of the pons. Tumors with exophytic expansion were excluded. There were 16 females and 20 males, ranging in age from 2 to 13 years, median 6 years. The most common presenting symptoms were cranial nerve dysfunction. unsteadiness of gait, and hemiparesis. Computed tomography (CT) showed a hypodense (17/21) or isodense (4/21) expansion of the pons. Five tumors had areas of contrast enhancement. Following information about prognosis and possible types of management, parents decided for or against radiation therapy: twentyfour children underwent irradiation and 12 did not. Median survival among children receiving a full course of irradiation was 280 days, compared to 140 days in an equivalent group of non-irradiated children. Hemiparesis presenting without cranial nerve symptoms and contrast enhancement on CT scan were poor prognostic factors, whereas sex, age, and duration of symptoms at diagnosis were unrelated to prognosis. 相似文献
2.
J W Petersen A Holm P H Ibsen K Haslv C Capiau I Heron 《Infection and immunity》1992,60(10):3962-3970
Ten adult humans were vaccinated with the Japanese acellular pertussis vaccine JNIH-3, containing detoxified pertussis toxin (PT), formaldehyde, and filamentous hemagglutinin. The vaccination induced a specific antibody response to PT and filamentous hemagglutinin, and a Western blot (immunoblot) analysis of the antibody response to PT revealed antibodies to PT subunits S1, S2, S3, S4 and S5. The response of peripheral lymphocytes to PT was assessed in an in vitro proliferation assay. A proliferative response to detoxified PT and PT dimers S2-S4 and S3-S4 was found, and it was further demonstrated that the proliferative response to detoxified PT and dimer S2-S4 was mediated by T cells of the CD4+ phenotype. The specificity of the proliferative response to subunit S4 was analyzed with a range of synthetic peptides synthesized on the basis of the primary sequence of subunit S4. The proliferative response to the peptides revealed two major and one minor T-cell epitope located in the NH2-terminal end of subunit S4. 相似文献
3.
Francis J Podbielski Heron E Rodriguez Andrew M Brown Matthew J Blecha Mario R Salazar Mark M Connolly 《JSLS, Journal of the Society of Laparoendoscopic Surgeons》2004,8(3):213-216
Management of an indeterminate pulmonary nodule is a diagnostic challenge that commonly confronts primary care physicians and specialists. Patients often present with this radiographic finding in the course of an unrelated medical evaluation. We examined our institution's experience with percutaneous biopsy of lung nodules to determine the impact of this procedure on overall patient care. Although significant complications are uncommon, the expedience of percutaneous lung biopsy often supplants a surgical opinion prior to initiation of therapy without added diagnostic benefit or cost-savings. Hence, we caution practitioners to use this technique as an adjunct to diagnosis and not a substitute for multidisciplinary care. 相似文献
4.
Hodgkin disease: CT of the thymus 总被引:2,自引:0,他引:2
The computed tomography (CT) scans in two groups of patients with Hodgkin disease were reviewed to determine the frequency of thymic enlargement. In 50 CT scans from 50 patients with evidence of thoracic disease on CT scans who were examined for primary staging, the thymus was enlarged in 15 of 50 (30%). Fifty CT scans were obtained from 44 patients at the time of 50 separate episodes of known or suspected relapse. Relapse occurred in the mediastinum in 12 episodes, lung parenchyma in five, and both sites in one. Thymic enlargement thought to be due to involvement by disease was present in seven of 18 (38%). Mediastinal disease was associated with thymic enlargement in all but one patient in whom a thymic cyst developed after radiation therapy. Differentiation of thymic enlargement from enlarged superior mediastinal lymph nodes was easily made in all but two patients. Thymic enlargement in the absence of lymph node enlargement may indicate a different disease, since isolated Hodgkin disease of the thymus is uncommon. Primary thymic tumor should be considered initially, whereas after treatment, rebound hyperplasia of the thymus may be the cause of enlargement. 相似文献
5.
6.
Henrik Aggerbeck Bent Nørgaard-Pedersen Iver Heron 《Journal of immunological methods》1996,190(2):171-183
A dual, double antigen, time-resolved fluorescence immunoassay (DELFIA) for the simultaneous detection and quantitation of diphtheria (D) and tetanus (T) antibodies in sera has been developed. In the double antigen format one arm of the antibody binds to antigen coated microtitre wells and the other arm binds to labelled antigen to provide a fluorescent signal. This assay was found to be functionally specific for IgG antibodies and showed a good correlation with established toxin neutralization assays. Furthermore, the double antigen set-up was species independent, permitting the direct use of existing international references of animal origin to measure protective antibody levels in humans in international units (IU/ml). The detection limit corresponded to 0.0003 IU/ml with Eu3+-labelled toxoids and to 0.0035 IU/ml using Sm3+-labelled toxoids. The assay was fast with a high capacity making it a suitable method for serological surveillance studies. 相似文献
7.
Specificity of a protective memory immune response against Mycobacterium tuberculosis. 总被引:7,自引:11,他引:7
下载免费PDF全文
![点击此处可从《Infection and immunity》网站下载免费的PDF全文](/ch/ext_images/free.gif)
We have investigated the memory T-cell immune response to Mycobacterium tuberculosis infection. C57BL/6J mice infected with M. tuberculosis were found to generate long-lived memory immunity which provided a heightened state of acquired resistance to a secondary infection. The T-cell response of memory immune mice was directed to all parts of the bacilli, i.e., both secreted and somatic proteins. Major parts of the memory T-cell repertoire were maintained in a highly responsive state by cross-reactive restimulation with antigens present in the normal microbiological environment of the animals. A resting non-cross-reactive part of the memory repertoire was restimulated early during a secondary infection to expand and produce large amounts of gamma interferon. The molecular target of these T cells was identified as a secreted mycobacterial protein with a molecular mass of 3 to 9 kDa. 相似文献
8.
Incidence of chromosomal imbalances in advanced colorectal carcinomas and their metastases 总被引:6,自引:0,他引:6
Knösel T Petersen S Schwabe H Schlüns K Stein U Schlag PM Dietel M Petersen I 《Virchows Archiv : an international journal of pathology》2002,440(2):187-194
Comparative genomic hybridization (CGH) was used to screen 54 advanced colon carcinomas. i.e., 24 primary tumors and 30 metastases, for chromosomal alterations. Using a sensitive statistical method for the determination of DNA imbalances and histograms for analysis of the incidence of changes, we identified the DNA over-representation of chromosome 20q as the most common alteration being present in 100% of cases. High incidence deletions were observed on 18q21-18q23 (96%), 4q27-4q28 (96%), 4p14 (87%), 5q21 (81%), 1p21-1p22 (72%), 21q21 (74%), 6q16 (72%), 3p12 (66%), 8p24-8p21 (66%), 9p21 (64%), 11q22 (64%), and 14q13-14q21 (64%). Further frequent over-representation was found on 7q12-7q11.2 (75%), 16p11-16p12 (70%), 19p13 (70%), 9q34 (67%), 19q13 (67%), 13q34 (64%), 13q13 (64%), 17q21 (59%), 22q11 (61%), 8q24 (57%), and 1q21 (57%). Pronounced DNA gains and losses being defined as regions in which the ratio profiles exceeded the values of 1.5 and 0.5, respectively, frequently colocalized with peaks of incidence curve. The use of difference histograms for the comparison of tumor subgroups as well as case-by-case histogram for the analysis of 15 paired tumor samples identified several of the above alterations as relevant for tumor progression and metastasis formation. The study identified additional loci and delineates more precisely those that have been previously reported. For comparative purposes, we have made our primary data (ratio profiles, clinicopathological parameters, histograms) available at the interactive web site http://amba.charite.de/cgh, where the incidence of changes can be determined at individual loci and additional parameters can be applied for the analysis of our CGH results. 相似文献
9.
A. S. Eadie J. R. Pugh B. Winn G. Heron 《Medical & biological engineering & computing》1988,26(2):143-146
The paper describes a low-cost system for the measurement of ocular accommodation. Digitisation of the low-frequency signals
obtained from the measurement is straightforward and high-speed methods are not necessary. The methods described are, therefore,
of interest to workers involved in the study of low-frequency signals, which are common in biological systems. 相似文献
10.
Schewe C Goldmann T Grosser M Zink A Schlüns K Pahl S Ulrichs T Kaufmann SH Nerlich A Baretton GB Dietel M Vollmer E Petersen I 《Virchows Archiv : an international journal of pathology》2005,447(3):573-585
The present study is based on the initiative for quality assurance in pathology of the German Society of Pathology and the Professional Association of German Pathologists. Four panel laboratories with experience and expertise in polymerase chain reaction (PCR) detection of Mycobacterium tuberculosis were selected to establish the prerequisites for continuous external laboratory trials, in particular, by providing pre-tested specimens and evaluation criteria for participating institutes. In the first step, the four panel laboratories performed an internal trial to test their own reliability and reproducibility. Paraffin sections and DNA preparations from 34 tissues (25 clinical specimens and 9 controls) totalling to 66 samples were evaluated by each panel institute according to their own protocols. The methodologies differed and are described in detail. Despite these differences, a high degree of inter-laboratory reliability was achieved. In this report, we summarise our results including the correlation with the histology and provide recommendations for applying PCR-based methodology for the detection of mycobacterial DNA in surgical specimens. Supplementary data are available online at (rubric Forschung). Pre-tested specimens are now available for the external trial and can be ordered from the steering institute via Oligene (). All molecular pathology laboratories are invited to participate in this quality assurance initiative. 相似文献