A Method for Assessing the Kinetics of Evoked Secretion of Transmitter Quanta Determining the Generation of Multiquantum Endplate Currents

Neuroscience and Behavioral Physiology -     

The Effects of Noradrenaline on the Amplitude-Time Characteristics of Multiquantum Endplate Currents and the Kinetics of Induced Secretion of Transmitter Quanta

Experiments on frog neuromuscular junctions using a two-electrode membrane potential clamping method were used to study the effects of noradrenaline on the amplitude-time characteristics of multiquantum endplate current (EPC) parameters and the time course of secretion of transmitter quanta during the process of EPC generation. Noradrenaline (10 M) induced significant increases in EPC amplitude (by 16%), with a decrease in the ratio of the duration of the leading front of the EPC to the duration of the leading front of the miniature endplate current (mEPC). Analysis of the time course of induced secretion, based on sequential subtraction of signals with displacement on the time scale, showed that noradrenaline induced synchronization of the process of secretion of quanta involved in generating multiquantum EPC, resulting in a 25% decrease in parameter P₉₀, which characterizes the extent of synchronization of quantum release. The quantum composition of EPC, measured by dividing the area of induced and spontaneous signals and by analysis of the time course of the secretion of quanta, showed no changes in response to noradrenaline. Thus, in conditions in which responses to single stimuli applied to the motor nerve results in the release of several tens of quanta, noradrenaline can lead to increases in the amplitude of multiquantum EPC by increasing the level of synchronization of secretion of the transmitter quanta forming this signal.     

Characteristics of the Time Course of Evoked Secretion of Transmitter Quanta in Different Parts of the Motor Nerve Ending in the Frog

Experiments were performed on neuromuscular preparations from frogs, in which three extracellular microelectrodes were used to record nerve ending currents and single-quantum endplate currents simultaneously from the proximal, central, and distal parts of single synaptic contacts. The rate of propagation of excitation across terminals was measured, along with the minimum synaptic delay, the intensity, and the degree of synchronicity of the secretion of transmitter quanta in different parts of the nerve ending, and the relationships between these factors and the calcium ion concentration in the medium. These studies showed that along with gradients in the rate of conduction of excitation and the intensity of secretion in different parts of the ending, there were also differences in the kinetics of the release of transmitter quanta. As the distance from the end of the myelinated part of the axon increased, the rate of conduction of the nerve impulse and the duration of the synaptic delay decreased, while the synchronicity of the release of quanta increased. Increases in the calcium concentration in the medium produced greater increases in the synchronicity of transmitter quantum release in the distal parts of the synapse than in the proximal parts. Mathematical modeling of multiple-quantum endplate currents showed that the characteristics of the kinetics of the secretion process observed here in different parts of the nerve ending represent a factor which partially compensates for the decrease in the amplitude and extending of the duration of the leading front of the multiple-quantum endplate current which are associated with the low rate of conduction of excitation across the nerve ending. The contribution of this compensation increases as the intensity of secretion of transmitter quanta increases in the distal parts of the synaptic contact.