大鼠马桑内酯急性局灶型癫痫大脑运动皮质的形态计量研究

用微量马桑内酯注入Ｗｉｓｔａｒ大鼠左侧前肢运动皮质，造成急性局灶型癫痫。用光镜、电镜和体视学方法研究其运动皮质第Ｖ层结构的改变。结果显示：癫痫大鼠运动皮质灶区、灶旁区的神经细胞数和胶质细胞数均分别比对照大鼠灶区和灶旁区显著减少；灶区神经毡中突触性终末数，显著减少；突触性终末的面积分数明显减少，而树突的面积分数无变化；神经胶质突起的面积分数增加。     

免疫透射比浊法测定血清免疫球蛋白

本文报道免疫透射比浊法测定血清免疫球蛋白,批内CV小于2.0％,批间CV小于3.5％,表明该法简便,快速,精密度和准确度高,重复性好。     

氨咖黄敏片人工牛黄中的贝斯素薄层色谱鉴别

目的:建立一种测定氨咖黄敏片人工牛黄中的贝斯素的薄层色谱鉴别方法.方法:采用TLC鉴别方法.结果:通过改变提取方法和展开剂获得清晰、持久的TLC图谱.结论:本法简便,灵敏,准确,重现性好,能有效地控制氨咖黄敏片的质量.     

四川省德阳市城市居民脑卒中监测10年动态分析

目的　研究城市居民脑卒中发病的动态变化趋势。　方法　分析四川省德阳市居民 10年脑卒中发病监测资料。　结果　连续监测 10年 (1991年～ 2 0 0 0年 )发病呈上升趋势 ,总计发病 791例 ,年均发病率为 76 4 6 10万 ;男性年均发病率为 89 6 9 10万 ,女性年均发病率为 6 1 13 10万 ,男性高于女性 ,男女发病之比为 1 70∶1。脑卒中发病随年龄的增加而升高。主要集中在 5 0岁以上人群。全死因监测显示心脑血管病已从“八·五”期间的第二位升至“九·五”期间的第一位 ,脑卒中则居心脑血管病的首位。　结论　我市脑卒中处于低发但呈快速上升趋势。应加强以控制高血压、改变生活行为方式、改善社区卫生服务等综合防治措施 ,以遏制脑卒中发病的快速上升     

CD147-CD98hc Complex Contributes to Poor Prognosis of Non-Small Cell Lung Cancer Patients Through Promoting Cell Proliferation Via the PI3K/Akt Signaling Pathway

Background

It has been reported that CD147 and CD98 heavy chain (CD98hc) form a complex on the cell plasma membrane of several cancers; however, whether this complex exists in non-small cell lung cancer (NSCLC) cells and affects the prognosis of patients remains to be elucidated.

Methods

The expression of CD147 and CD98hc was assessed in tissue samples from 241 NSCLC patients and NSCLC cell lines. The correlation between CD147 and CD98hc expression and their association with the prognosis of NSCLC patients were analyzed. We also evaluated the impact of CD147 and CD98hc on the growth of NSCLC cells as well as Akt phosphorylation.

Results

Both CD147 and CD98hc were significantly upregulated in NSCLC cells, and their expression levels were significantly correlated (p < 0.001). Immunoflurenece staining and co-immunoprecipitation demonstrated that CD147 and CD98hc could form a complex on NSCLC cells. Compared with NSCLC patients with CD147?/CD98hc?, those with CD147+/CD98hc+ exhibited a significantly poor overall survival (OS) with a hazard ratio (HR) of 1.92 (p = 0.010), and a significantly increased risk of recurrence with a HR of 1.97 (p = 0.004). Also, we demonstrated that the proliferation of lung cancer cell lines was significantly affected by knockdown and force-expression of the CD147-CD98hc complex. Western blot analysis indicated that the phosphorylation of Akt in NSCLC cells was significantly affected by knockdown and overexpression of either or both CD147 and CD98hc.

Conclusions

Our findings indicate that the CD147-CD98hc complex significantly contributes to poor prognosis of NSCLC patients through promoting cell proliferation via the PI3K/Akt pathway.     

广州地区新型冠状病毒肺炎的高分辨率CT表现与临床特点

目的 初步探讨广州地区新型冠状病毒肺炎(COVID-19)的临床及首诊高分辨率CT(HRCT)影像表现特点。方法 回顾性分析2020年1月22日至30日广州市第八人民医院确诊的91例COVID-19患者,其中男39例、女52例,中位年龄50(33, 62)岁,分析91例COVID-19患者的临床及HRCT特点。结果 临床表现主要为发热70例,咳嗽57例,以干咳为主(39例)。首次HRCT有24例COVID-19患者呈阴性表现,67例HRCT有异常病灶,主要表现为肺内磨玻璃影65例、病灶内合并血管束增粗64例、病灶邻近胸膜增厚50例、间质间隔增厚47例、斑片影42例。所有患者均未见肿大淋巴结。病灶分布:双肺弥漫分布的2例,多发57例,单肺叶出现8例;病灶主要分布于胸膜下46例,肺下叶分布为著39例,上叶为著7例,在肺内无明显分布特征的有13例。结论 广州地区COVID-19早期影像主要表现为多发磨玻璃影,以胸膜下及下肺野多见,多有肺间质间隔的增厚,广州地区COVID- 19轻型及普通型患者比例较高,部分确诊患者首次HRCT呈阴性表现,对于HRCT呈阴性的患者,应及时复查。     

Functional Brain Network Classification for Alzheimer’s Disease Detection with Deep Features and Extreme Learning Machine

The human brain can be inherently modeled as a brain network, where nodes denote billions of neurons and edges denote massive connections between neurons.     

创建学习型医院 加快医院发展

创建学习型医院是实践"三个代表"重要思想,实现人的全面发展的迫切需要,是顺应时代潮流、紧跟时代脉搏的重要举措,是全面提升医院核心竞争力、保持医院可持续发展的内在要求.加强领导,提高创建学习型医院的认识,开展形式多样的主题教育活动,以丰富多彩的活动为载体,寓学习于工作中,寓教育于活动中,激励岗位成才,为职工构筑学习环境,是创建学习型医院的有力措施.     

创伤性湿肺的X线与CT诊断

目的 探讨X线摄影、CT在创伤性湿肺诊断中的应用价值及其临床意义。方法 回顾分析21例创伤性湿肺病例的X线、CT影像资料，并加以总结。结果 创伤性湿肺主要表现为肺纹影增强、模糊，占85.71%；肺内斑片影，占71.43%；面纱征或／和毛玻璃征，占23.81%；肺血肿影，占14.29%及其他如肋骨骨折、胸腔积液、膈肌破裂等影像表现。结论 X线摄影、CT检查目前仍是诊断创伤性湿肺的主要手段。