CD19 + CD21lo/neg cells are increased in systemic sclerosis-associated interstitial lung disease

Interstitial lung disease (ILD) represents a significant cause of morbidity and mortality in systemic sclerosis (SSc). The purpose of this study was to examine recirculating lymphocytes from SSc patients for potential biomarkers of interstitial lung disease (ILD). Peripheral blood mononuclear cells (PBMCs) were isolated from patients with SSc and healthy controls enrolled in the Vanderbilt University Myositis and Scleroderma Treatment Initiative Center cohort between 9/2017–6/2019. Clinical phenotyping was performed by chart abstraction. Immunophenotyping was performed using both mass cytometry and fluorescence cytometry combined with t-distributed stochastic neighbor embedding analysis and traditional biaxial gating. This study included 34 patients with SSc-ILD, 14 patients without SSc-ILD, and 25 healthy controls. CD21^lo/neg cells are significantly increased in SSc-ILD but not in SSc without ILD (15.4 ± 13.3% vs. 5.8 ± 0.9%, p = 0.002) or healthy controls (5.0 ± 0.5%, p < 0.0001). While CD21^lo/neg B cells can be identified from a single biaxial gate, tSNE analysis reveals that the biaxial gate is comprised of multiple distinct subsets, all of which are increased in SSc-ILD. CD21^lo/neg cells in both healthy controls and SSc-ILD are predominantly tBET positive and do not have intracellular CD21. Immunohistochemistry staining demonstrated that CD21^lo/neg B cells diffusely infiltrate the lung parenchyma of an SSc-ILD patient. Additional work is needed to validate this biomarker in larger cohorts and longitudinal studies and to understand the role of these cells in SSc-ILD.

     

French adaptation and validation of the Carroll rating scale for depression

The authors translated the Carroll Rating Scale for depression published in 1982. They evaluated the reliability of this scale in 50 normal volunteers. They validated the French Version against the Hamilton Rating Scale and the Beck Depression Inventory. The translation has been shown to be reliable. The correlation between the Hamilton Rating Scale and the Carroll Rating Scale is better than the one between the Hamilton Rating Scale and the Beck Depression Inventory. The Carroll Rating Scale appears to be useful for a quick evaluation of depressive symptoms.     

White spot syndrome virus and infectious hypodermal and hematopoietic necrosis virus simultaneous diagnosis by miniarray system with colorimetry detection

Highly sensitive and specific diagnostic tools are essential for monitoring the health status of farmed species. After the development of genomic probe diagnostic systems in the 1990s, followed by PCR-based systems, a miniarray method has been developed allowing one-step multiple detection. The miniarray method was developed to enable the accessibility of powerful array technology. To use this system, hybridisation and washing process were modified, resulting into a significant increase in the test's rapidity and sensitivity. With miniarray technology, hybridisation time is reduced to 20 min, whereas other methods require a longer hybridisation time. Hybridisation of the PCR product on a nylon membrane and revelation of the hybrids by an antibody increase considerably the ability of pathogen's detection. A first application is developed for the diagnosis of two specific viruses which are, by their geographical range and their impact on the production, very important in shrimp pathology, namely, the White Spot Syndrome Virus (WSSV) and the Infectious Hypodermal and Hematopoietic Necrosis Virus (IHHNV).     

Overt and covert personality traits associated with coronary heart disease.

Some authors suggested the existence of contradictory traits in the personality pattern associated with coronary heart disease: while presenting overt, active, adult-like traits, coronary subjects would be, at a more covert or repressed level, characterized by passive and infantile tendencies. To test this hypothesis, coronary and control subjects were submitted to three types of personality questionnaire, each of them measuring the same four personality traits (seclusion, impulsiveness, dependence and passivity) which, in the adult individual, are considered by Murray's (1938) theory of personality as persisting from infancy. No difference appeared between the two groups on type 1 questionnaires, describing behavioural features of individuals outwardly displaying the four traits. On type 2 questionnaires, describing tastes and similar areas less subject to social norms, coronary subjects revealed themselves higher than control subjects for passivity (P less than 0.05). and dependence (P less than 0.05). Similarly, they were higher for passivity (P less than 0.05), dependence (P less than 0.001) and impulsiveness (P less than 0.05) on type 3 questionnaires describing symptoms expected to occur occasionally among adults having such traits. Supplementary scales also showed coronary subjects to be more ego-defensive (P less than 0.001) and more self-assertive (P less than 0.05) than controls. These data were considered as supporting the hypothesis.