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BACKGROUND: Tropomyosin 4 level has been found to be an increase in the spinal cord based on the 2-DE/MALDI-TOF/MS method. However, there is little report about the relationship between tropomyosin 4 and pathogenesis and progress of spinal cord injuries.
METHODS/DESIGN:Randomized controlled trial: rat models of complete spinal cord transection were made and expression levels of tropomyosin 4 at 3-28 days after modeling were determined by two-dimensional electrophoresis, animo acid serie analysis, quantitative PCR and western blot. Experiment for exporing the genetic mechanism: effects of tropomyosin 4 scilencing by lentivirus recomnination technology on the dendrite length of spinal cord neurons in vitro were observed, and its effects on the neurological function of rats after complete spinal cord transaction were assessed through Basso, Beattie, and Bresnahan scoring.
DISCUSSION: This study will be powered to provide a novel and effective treatment strategy for neurological function recovery after spinal cord transection based on the lentivirus recomnination carrying tropomyosin 4, as well as optimistic future for clinical gene treatment of complete spinal cord transaction through figuring out the underlying mechanism.
ETHICAL APPROVAL: This study was approved by the Ethics Committee of Kunming Medical University, China. The surgical operation and postoperative care of rats were in line with the rules of Chinese Experimental Animal Protection and Ethics Committee, and the guideline of the National Institutes of Health
中国组织工程研究杂志出版内容重点：组织构建;骨细胞;软骨细胞;细胞培养;成纤维细胞;血管内皮细胞;骨质疏松;组织工程 相似文献
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BACKGROUND:Bacillary dysentery is one of the most common enteric infectious diseases in summer and autumn that is mainly caused by Shigella.
OBJECTIVE: To analyze the pathological features of the rat ileum after Shigella flexneri infection.
METHODS: Totally 30 male Sprague-Dawley rats were enrolled and equally randomized into three groups. Rats in non-streptomycin and streptomycin groups were firstly treated with normal saline and 50 g/L streptomycin sulphate via gastric lavage, and then given gastric lavage of 9×108 CFU/mL Shigella flexneri. Rats were given no any treatment as control group. 14 days later, morphological changes of the rat ileum were observed through hematoxylin-eosin staining.
RESULTS AND CONCLUSION: (1) After Shigella flexneri infection, the rat distal ileum and rectum were characterized by mucosal edema and hemorrhage, neutrophilic granulocyte exudation, pseudomembrane formation and destruction of goblet cells with hemorrhage. There were obvious fibrin exudation on the necrotic mucosal surface that constituted the pseudomembrane with necrotic debris, leukocytes and erythrocytes. After mucosa defulvium, non-uniform and superficial ulcers were mainly visible at the mucosa. (2) The occurrence time of positive systems, temperature and body mass before and after Shigella flexneri infection showed no significant differences among rats (P > 0.05). (3) These results suggest that the rat distal ileum and rectum after Shigella flexneri infection present the characteristic morphological futures of bacillary dysentery; therefore, it can be used as the animal model to undergo in-depth research about the etiology, pathogenesis and treatment of bacillary dysentery.
中国组织工程研究杂志出版内容重点：肾移植;肝移植;移植;心脏移植;组织移植;皮肤移植;皮瓣移植;血管移植;器官移植;组织工程 相似文献
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