排序方式: 共有13条查询结果,搜索用时 15 毫秒
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高效液相色谱法测定复方天麻益阴酒中天麻素的含量 总被引:1,自引:0,他引:1
目的:建立复方天麻益阴酒中天麻素的高效液相色谱含量测定方法。方法:C18色谱柱(4.6mm×150mm,5μm),流动相为乙腈-0.05%磷酸溶液(3:97),流速1.0mL/min,检测波长220nm。结果:天麻素与样品中其它组分分离效果较好,天麻素在0.0992~1.4880μg范围内具有良好线性关系(r=0.9999),平均回收率为97.3%(RSD=0.83%,n=6)。结论:采用此方法测定复方天麻益阴酒中天麻素的含量,准确可靠,简便易行。 相似文献
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Li Zhanxia Zhou Songwen Zhang Ling Su Chunxia Hang Jinqin Zhao Yinmin Su Bo Zhou Caicun 《Medical oncology (Northwood, London, England)》2011,28(2):572-577
We sought to improve the understanding of oncogene-dependent and independent non-small-cell lung cancer (NSCLC), which could
provide insight into mechanism of sensitivity and/or resistance to tyrosine kinase inhibitors or chemotherapeutics. NSCLC
cell lines with different EGFR genotypes were used in this study; MTT assay and flow cytometry were applied to study the sensitivities
of these cell lines to gefitinib and cisplatin. Western blot was performed to determine the expression levels of BIM and other
Bcl-2 family proteins pre- and pro-treatment. Gefitinib provoked apoptosis of caspase activation via the intrinsic pathways
and significantly up-regulated expression of BIM protein in drug-sensitive PC-9 cell line, but not resistant PC-9/BB4 cell
line. The knockdown of BIM expression by RNA interference virtually eliminated gefitinib-induced cell killing in PC-9 cells
in vitro. Cisplatin could induce apoptosis of the cell lines, including H1299, A549, PC-9, and PC-9/BB4 cells, but which was
not associated with overexpression of BIM. BIM is involved in TKI-induced apoptosis in sensitive EGFR-mutant cell line. Down-regulation
of BIM and resistance to gefitinib were both seen in the acquired resistant PC-9/BB4 cell line. The induction of BIM may have
a role in the treatment of TKI-resistant tumors. 相似文献
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糖尿病下肢深静脉血栓形成的相关因素分析及护理对策 总被引:1,自引:0,他引:1
目的 探讨糖尿病(DM)下肢深静脉血栓形成(DVT)的相关因素并制订临床护理措施.方法 对58例DM行双下肢99mTc-MAA深静脉显像(和/或超声检查),同期获取空腹血糖、血胆固醇,血三酰甘油等实验室资料及年龄、病程、下肢症状(肿胀、静脉曲张和DM足)等临床资料.分析各因素与DVT发生的关系.结果 28例(48.3%)DM并发DVT;年龄、病程、血糖、血胆固醇、血三酰甘油、下肢症状与下肢DVT发病率有关(P<0.05,P<0.01),血压、体重指教、糖尿病类型与DVT发病率关系不大(均P>0.05).结论 高龄、长病程、高血糖、高胆固醇、高三酰甘油等危险因素相互累积易致下肢DVT,应尽早采取综合治疗措施,加强护理,以减少DVT并发症的发生. 相似文献
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目的:建立同时测定丹红注射液中丹参素和原儿茶醛含量的超高效液相色谱法。方法:采用BEH ShieLd PR18色谱柱(2.1 mm ×100.0 mm,1.7μm),以甲醇-(体积分数)0.5%冰醋酸水溶液(11:89)为流动相,流速0.2 mL·min-1,检测波长279 nm,柱温30℃。结果:丹参素和原儿茶醛的峰面积与含量呈良好的线性关系( r=0.9999),两种成分的回收率分别为100.03%和99.22%,RSD值分别为0.08%和0.59%,三批丹红注射液中丹参素的含量为1.4599~1.4759 g·L-1,原儿茶醛的含量为0.2290~0.2360 g·L-1。结论:所建立的丹红注射液中丹参素和原儿茶醛含量的超高效液相色谱法简便、准确、快速,为该制剂的质量控制提供了有效手段。 相似文献
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目的探讨不同浓度大黄酚对原代培养小鼠星形胶质细胞活性及谷氨酸代谢功能的影响。方法原代培养小鼠星形胶质细胞,大黄酚10、20、40、60、80、100、200、250 mg/L处理细胞1、2、3d,MTT法检测大黄酚对星形胶质细胞活性的影响;RT-PCR检测谷氨酸/天门冬氨酸转运体(GLAST)及谷氨酸转运体1(GLT-1)mRNA表达水平。相关试剂盒检测谷氨酰胺合成酶(GS)和谷胱甘肽过氧化物酶(GSH-Px)活性。结果大黄酚可抑制星形胶质细胞活性,可诱导星形胶质细胞GLAST和GLT-1 mRNA表达上调,GSH-Px和GS活性增加,且均具有时间和浓度依赖性。但相似浓度且作用时间相同的大黄酚并不增加细胞的活性,即大黄酚对细胞外谷氨酸浓度的影响并不依赖细胞数量或活性的增加。结论大黄酚可抑制小鼠星形胶质细胞活性,对谷氨酸代谢的调节具有时间和浓度依赖性。 相似文献
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Dongyi Zhu Li Gu Zhanxia Li Wenjing Jin Qingchun Lu Tao Ren 《Pathology, research and practice》2019,215(5):861-872
Background
MiR-138-5p is regarded as a tumour suppressor in many cancers. Transforming growth factor beta (TGF-β) often acts as a tumor promotor at the late stages of human cancers. However, the function of miR-138-5p on lung adenocarcinoma cells induced by TGF-β remains to be further confirmed.Methods
RT-qPCR was used to detect the expression of human lung adenocarcinoma tissues, adjacent normal tissues, and relative cell lines. When the lung adenocarcinoma cells A549 and H1299 were transfected with negative control (NC), miR-138-5p mimics and miR-138-5p inhibitor by lipofectamine3000 and treated with or without TGF-β1, the lung adenocarcinoma cell function was detected by Immunofluorescence, Western blotting (WB), cell counting Kit-8 (CCK8), colony formation, EdU, Wound-healing and Transwell assays. The relation between miR-138-5p and zinc finger E-box-binding homeobox 2 (ZEB2) was detected by RT-qPCR, WB, and Luciferase reporter assays. When ZEB2 was knocked down, the lung adenocarcinoma cell function was detected by WB, CCK8 and Transwell assays.Results
The expression of miR-138-5p was decreased in lung adenocarcinoma tissues and cell lines. When treated with or without TGF-β1, overexpression of miR-138-5p suppressed EMT, proliferation and metastasis of A549 and H1299. ZEB2 was verified as the direct target of miR-138-5p. Downregulation of ZEB2 suppressed EMT, proliferation and metastasis of lung adenocarcinoma cell, which could be reversed by miR-138-5p inhibitor.Conclusions
MiR-138-5p inhibits epithelial-mesenchymal transition, growth and metastasis of lung adenocarcinoma cells through targeting ZEB2. 相似文献9.
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近年来随着超声仪器和技术的发展,冠状动脉血管内超声(IVUS)不仅可以显示血管壁的形态和病变性质,而且可以通过二维平面分析和三维重建对病变大小进行精确测量,为深入了解血管病变的形态和功能提供了新的视野,也为指导临床诊断和治疗提供了更加翔实可靠的信息。我院2013年5月~2014年5月,对20例冠心病患者行冠状动脉造影术检查,对这些患者20处血管行冠状动脉血管内超声(IVUS)检查,取得满意的效果,而实施护理措施的密切配合起到了重要作用。 相似文献