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排序方式: 共有375条查询结果,搜索用时 62 毫秒
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K Kunishio T Maeshiro T Matsuhisa N Mishima K Tsuno H Shigematsu K Matsumoto T Furuta A Nishimoto 《No shinkei geka. Neurological surgery》1992,20(1):39-44
The correlation between the extent of peritumoral edema and the proliferative potential or the infiltration of mononuclear cells was studied in 17 gliomas. The peritumoral edema was evaluated on contrast enhanced CT scan as the ratio of the low density area around the tumor to the enhanced high density area. The proliferative potential and the infiltration of mononuclear cells into the tumor were investigated immunohistochemically using monoclonal antibody (MAb) against DNA polymerase alpha and anti-Leu MAb's respectively. There was a significant correlation between the extent of the peritumoral edema and the percentage of DNA polymerase alpha positive cells. The degree of the infiltration of mononuclear cells into the tumor tissue also correlated with the extent of peritumoral edema. In gliomas with high proliferative potential and/or severe infiltration of mononuclear cells, the peritumoral edema may be aggravated by disruption of the blood-brain-barrier and increased vascular permeability. 相似文献
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Y Tsuno 《[Zasshi] [Journal]. Nihon Kyōbu Geka Gakkai》1989,37(12):2439-2448
Radical operation for patients with thoracic esophageal cancer is one of the surgical procedures with the most severe surgical stress in the digestive system because of frequent serious postoperative complications particularly with respiratory problems. For the purpose of reducing surgical influences and postoperative pulmonary complications in patients with esophageal cancer, we have been insisting on "one-stage operation" with preceding reconstruction of the esophagus on the basis of our clinical experiences and model experiments. In the model experiments minimizing radical operation for thoracic esophageal cancer, the increase of intra-operative and post-operative pulmonary water content in the laparotomy-preceding group (LPG) is lesser than in the thoracotomy-preceding group (TPG). This results were also supported by histological findings. In the TPG, a tendency of pulmonary water accumulation was accelerated with an increase of surgical influences and, in addition, pulmonary water accumulation was noted even in the lung of the opposite side. Furthermore, the host defense reaction to the surgery and the influences of respiratory and circulatory systems were observed predominantly in this group. It is concluded that one-stage operation with preceding reconstruction of the esophagus shows a rationality in reducing the surgical influences to the body, especially to the pulmonary systems. 相似文献
5.
K Kunishio N Mishima T Matsuhisa K Tsuno N Matsumi T Satoh K Matsumoto T Furuta A Nishimoto T Shiraishi 《Journal of neurosurgery》1990,72(2):268-272
The proliferative capacity of brain-tumor cells was analyzed in vitro and in situ using monoclonal antibody (MAb) against deoxyribonucleic acid (DNA) polymerase alpha. For the in vitro studies, two cultured human glioma cell lines were investigated using MAb against DNA polymerase alpha, the MAb Ki-67, a serum against proliferating cell nuclear antigen (PCNA/cyclin), bromodeoxyuridine (BUdR), and an anti-BUdR MAb. During exponential growth of the cells, the percentage of polymerase alpha-positive cells (the "polymerase alpha score") ranged from 72.0% to 77.1%, the Ki-67-positive cells (the "Ki-67 score") ranged from 43.4% to 59.4%, the PCNA/cyclin-positive cells from 30.9% to 41.4%, and the BUdR labeling index from 28.6% to 39.3%. For the in situ studies, tissue from 60 human brain tumors and from two normal human brains was investigated and the polymerase alpha scores and Ki-67 scores were compared. In normal brain tissue, no immunostaining was found by either method. In brain tumors, both the polymerase alpha scores and the Ki-67 scores correlated with the histological grade of malignancy. Polymerase alpha scores were generally higher than Ki-67 scores in the same specimen, especially in malignant brain tumors. These findings suggest that immunostaining of DNA polymerase alpha is a convenient and important new method by which to estimate the cellular proliferation rate of brain tumors. Polymerase alpha scores may be closer to the growth fraction of the individual tumor than the MAb Ki-67 or other scores. 相似文献
6.
Suyama H Tsuno S Takeyoshi S Takiyama R 《Masui. The Japanese journal of anesthesiology》2000,49(7):765-767
An extremely low birth weight (832 g) and 29 gestational week neonate underwent surgical ligation of patent ductus arterious 20 days after birth and repair of coarctation of the aorta two months after the first operation. She developed asphyxia neonatorum and was artificially ventilated because of IRDS and attack of apnea. At the first operation, anesthesia was maintained with fentanyl and sevoflurane. The only perioperative complication was severe hypothermia (34.5 degrees C). At the second operation, anesthesia maintenance was identical to the first operation. The only perioperative complication was mild hyperthermia (37.7 degrees C). The key points of anesthetic management were the use of a low inspired oxygen fraction to avoid retrolental fibroplasia at a gestational age before 32 weeks and management of the baby's temperature. 相似文献
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Activated protein C decreases plasminogen activator-inhibitor activity in endothelial cell-conditioned medium 总被引:12,自引:0,他引:12
van Hinsbergh VW; Bertina RM; van Wijngaarden A; van Tilburg NH; Emeis JJ; Haverkate F 《Blood》1985,65(2):444-451
Confluent cultures of endothelial cells from human umbilical cord were used to study the effect of activated human protein C (APC) on the production of plasminogen activators, plasminogen activator-inhibitor, and factor VIII-related antigen. Addition of APC to the cells in a serum-free medium did not affect the production of tissue-type plasminogen activator (t-PA) or factor VIII-related antigen; under all measured conditions, no urokinase activity was found. However, less plasminogen activator-inhibitor activity accumulated in the conditioned medium in the presence of APC. This decrease was dose dependent and could be prevented by specific anti-protein C antibodies. No decrease was observed with the zymogen protein C or with diisopropylfluorophosphate-inactivated APC. APC also decreased the t-PA inhibitor activity in endothelial cell-conditioned medium in the absence of cells, which suggests that the effect of APC is at least partly due to a direct effect of APC on the plasminogen activator- inhibitor. High concentrations of thrombin-but not of factor Xa or IXa-- had a similar effect on the t-PA inhibitor activity. The effect of APC on the plasminogen activator-inhibitor provides a new mechanism by which APC may enhance fibrinolysis. The data suggest that activation of the coagulation system may lead to a secondary increase of the fibrinolytic activity by changing the balance between plasminogen activator(s) and its (their) fast-acting inhibitor. 相似文献
9.
Yasushi Okuaki Hiroshi Miyazaki Mikio Zeniya Tomohisa Ishikawa Yasuhiko Ohkawa Shinichi Tsuno Masami Sakaguchi Masaki Hara Hiroki Takahashi Gotaro Toda 《Liver international》1996,16(3):188-194
Abstract: In the present study, we investigated the role of the spleen in experimental hepatic ischemia/reperfusion in the rat. After a 90-min period of ischemia in the left and middle hepatic lobes, the ischemia was released and the liver was reperfused for up to 24 h. Plasma alanine aminotransferase reached a peak 3 h after the onset of reperfusion, and gradually decreased thereafter. A histological examination revealed evidence of hepatocellular necrosis and degeneration, especially 24 h after the onset of reperfusion. In addition, there was a noticeable accumulation of polymorphonuclear cells in the liver following ischemia/reperfusion. A splenectomy performed just prior to ischemia/reperfusion reduced both biochemical and histological hepatocellular injury. The number of polymorphonuclear cells in the liver following ischemia/reperfusion was significantly reduced in rats subjected to splenectomy, suggesting that the increase in polymorphonuclear cells may contribute to liver injury. The number of mononuclear cells also increased in the marginal zones of the spleen following ischemia/reperfusion, and appeared to be derived from the splenic monocyte/macrophage population, based on immunohistochemical studies. The spleen plays an important role in the pathogenesis of hepatic ischemia/reperfusion injury and the splenic monocyte/macrophage population contributes to liver damage. 相似文献
10.
Mari Terada Satoshi Kutsuna Tomiteru Togano Sho Saito Noriko Kinoshita Yumiko Shimanishi Tetsuya Suzuki Yusuke Miyazato Makoto Inada Takahito Nakamoto Hidetoshi Nomoto Satoshi Ide Mitsuhiro Sato Kenji Maeda Akihiro Matsunaga Masahiro Satake Keiji Matsubayashi Hirokazu Tsuno Makiko Kojima Madoka Kuramistu Kenta Tezuka Emi Ikebe Kazu Okuma Isao Hamaguchi Katsuyuki Shiratori Motohiko Sato Yuiko Kawakami Kumi Inaba Saori Igarashi Reina Yamauchi Mina Matsumura Keiko Ishimaru Bijuan Zhang Chika Kuge Maiko Ishihara Miho Gouda Keiko Tanaka Yukihito Ishizaka Norio Ohmagari 《Transfusion》2021,61(7):1998-2007