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排序方式: 共有114条查询结果,搜索用时 15 毫秒
1.
Jiang WZ Jin NY Li ZJ Zhang LS Wang HW Zhang YJ Han WY 《第二军医大学学报》2006,27(4):434-434
To express the core protein of HIV-1 of Chinese prevalent strain (HIV-1 (CN)) in Pichia pastoris, the fulllength gag gene was inserted into the secretory expression vector pHILS1. Linearized recombinant plasmid pHILGAG by Sail was electrotransformed into the yeast strain GS115, and the yeast transformants were identified by PCR. To induce the interest protein to be expressed, the PCR positive transformants were inoculated in the medium of BMGY and BMMY, mRNA of the strain was detected by RT-PCR, and the expressed protein was analyzed by SDS-PAGE, Western blotting and thin layer scanning. mRNA (1.3 kb) was amplified by RT-PCR. SDS-PAGE and Western blotting analysis showed that the molecular mass of the expressed protein was 55 kD, which was similar to the expected value, and the expressed protein could react with McAb to HIV-1 p24. Thin layer scanning analysis demonstrated that the whole amount of the expressed protein was approximately 13 % of the soluble protein in the supernatant. The recombinant yeast had good genetic stability. The optimal expression conditions of the engineering yeast were as follows: BMMY medium, 80-90% of dissolved oxygen, 1% methanol, and 3-day-cultivation course. Gag proteins were expressed under the optimal expression condition and purified via gel filtration chromatography. The purity of the interest protein was up to 85 %. After the purified proteins were inoculated into BALB/c mice, the anti-HIV-1 antibodies in the immunized mice could be detected by Western blotting. 相似文献
2.
Maries Joseph Taoufik Zoubeidi Sherina M. Al-Dhaheri Aysha Ahmed Al-Dhaheri Afra A. Al-Dhaheri Fatima M. Al-Kaabi 《The Journal of asthma》2013,50(2):175-178
Consanguinity is known to increase the burden of genetic disorders among offspring. However, the effect of consanguinity on a complex disorder like childhood asthma has not been studied previously. Therefore, we explored this relationship by studying the asthma prevalence in children between 6 and 14 years of age among the local Arab families of the United Arab Emirates (UAE) where consanguinity is known to be highly prevalent. A total of 1136 children from 295 families met our inclusion criteria. The prevalence of childhood asthma was higher among children in consanguineous families (43.3%) compared to non-consanguineous (22.6%, p < 0.001). There was a significant correlation between the degree of consanguinity and the number of asthmatic children per family (p = 0.0002). Girls from consanguineous families had proportionately more asthma (42.9%, p < 0.001) compared to boys (23.1%, p = 0.539).Paternal asthma in consanguineous families increased asthma risk for both boys and girls (p = 0.021 for boys, p < 0.001 for girls), while maternal asthma had no significant impact on asthma in offspring. Prevalence of childhood asthma was significantly higher in consanguineous families. The significant asthma predictors for girls from the consanguineous families were the degree of consanguinity and paternal asthma. The only predictor for boys was paternal asthma. These interesting observations merit further studies on both larger samples and in other consanguineous communities for confirmation. 相似文献
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目的对自闭症病人给予被动性语言刺激,评价采用功能性磁共振(MR)成像作为判断病人有无语言缺陷的客观指标的可行性。材料与方法本研究为前瞻性研究,研究方 相似文献
6.
Yulia M. Ulybina Ekatherina Sh. Kuligina Nathalia V. Mitiushkina Nathalia Yu. Sherina Grigoriy A. Yanus Tatiana V. Gorodnova Anna S. Katanugina Andrey V. Koloskov Alexandr V. Togo Evgeny N. Imyanitov 《Experimental gerontology》2010
Our previous studies, which included genotyping of multiple coding apoptotic gene polymorphisms, unexpectedly demonstrated a depletion of heterozygous CASP5 Ala90Thr (rs507879, c.268 G > A) genotypes in elderly subjects. Present investigation was aimed to validate this trend. An analysis of 510 subjects aged 75–103 years revealed 205 (40%) CASP5 Ala90Thr heterozygotes as compared to 254 (50%) expected from the minor allele frequency 0.470 (p = 0.000014). This deviation was not observed in 549 middle-aged (18–50 years) controls (270 (49%) heterozygotes observed vs. 274 (50%) expected; minor allele frequency 0.475; p = 0.743). Unfavorable significance of CASP5 heterozygous genotype may be explained by the role of the caspase-5 in inflammation-related processes. Almost all prior gene-longevity association studies focused on discrimination between “good” and “bad” gene variants. Here we present a distinct situation, where the combination of alternative alleles (i.e., heterozygosity) appears to be unfavorable as compared to the homozygous carriership of either gene variant. 相似文献
7.
Sokolenko AP Rozanov ME Mitiushkina NV Sherina NY Iyevleva AG Chekmariova EV Buslov KG Shilov ES Togo AV Bit-Sava EM Voskresenskiy DA Chagunava OL Devilee P Cornelisse C Semiglazov VF Imyanitov EN 《Familial cancer》2007,6(3):281-286
Previous studies indicate that founder mutations may play a noticeable role in breast cancer (BC) predisposition in Russia.
Here we performed a systematic analysis of eight recurrent mutations in 302 BC cases (St.-Petersburg, Russia), which were
selected due to the presence of clinical indicators of hereditary disease (bilaterality and/or early onset (≤40 years) and/or
family history). BC-associated alleles were revealed in 46 (15.2%) women. BRCA1 5382insC mutation was detected in 29 (9.6%)
patients, CHEK2 1100delC in 9 (3.0%), BRCA1 4153delA in 3 (1.0%), CHEK2 IVS2+1G>A in 2 (0.7%), and BRCA1 185delAG, BRCA2 6174delT
and NBS1 657del5 in 1 (0.3%) patient each. No cases with BRCA1 300T>G (C61G) mutation was identified. The obtained data suggest
that a significant fraction of hereditary BC cases in Russia can be diagnosed using only a limited number of simple PCR tests. 相似文献
8.
Sherina Mohd-Sidik Mehrnoosh Akhtari-Zavare Ummavathy Periasamy Lekhraj Rampal Siti Irma Fadhilah Rozi Mahmud 《Patient education and counseling》2018,101(5):862-871
Objectives
The aim of this study was to implement and evaluate the outcomes of chemotherapy counselling based on the “Managing Patients on Chemotherapy” module on self-esteem and psychological affect (anxiety, depression) of cancer patients by pharmacists in ten selected government hospitals in Peninsular Malaysia.Methods
A randomized control trial was conducted among 2120 cancer patients from April 2016 to January 2017 in ten selected government hospitals in Peninsular Malaysia. Cancer patients were randomly assigned to intervention and control groups. The intervention group received chemotherapy counselling by pharmacists based on the “Managing Patients on Chemotherapy” module. The outcomes were assessed at baseline, 1st, 2nd and 3rd follow-ups after counselling. In the course of data analysis; independent sample t-test, chi-square and two-way repeated measures ANOVA were conducted.Results
Mean scores of self-esteem in the intervention group had significant difference in comparison with those of the control group in the 1st, 2nd and 3rd follow-ups after counselling (P?<?0.0001). Also, among those with depression and anxiety at baseline, there was reduction in depression and anxiety scores after the 1st, 2nd and 3rd follow-ups after counselling (p?<?0.05).Conclusion
Repetitive counselling by pharmacists based on the “Managing Patients on Chemotherapy” module had positive effect on improving self-esteem and psychological affect of cancer patients undergoing chemotherapy in Peninsular Malaysia.Practice implications
This module can be used for all Malaysian cancer patients undergoing chemotherapy to improving self-esteem and psychological affect. 相似文献9.
The influence of pretransplant lipoprotein abnormalities on the early results of renal transplantation 总被引:3,自引:0,他引:3
E. DIMÉNY G. TUFVESON† H. LITHELL‡ E. LARSSON§ A. SIEGBAHN¶ B. FELLSTRÖM 《European journal of clinical investigation》1993,23(9):572-579
Abstract. Lipoprotein patterns were investigated before and after renal transplantation in a prospective study including 151 patients. Kidney graft losses during the first 6 months were associated with higher total cholesterol ( P = 0.03), LDL cholesterol ( P = 0.003) and LDL triglyceride levels ( P = 0.01) before transplantation. Patients with serum cholesterol ±6.9 mmol l-1 before transplantation had more acute rejections (1.7 vs. 0.9), a worse graft function and more vascular intimal hyperplasia and glomerular mesangial changes in transplant biopsies at 6 months. Patients with serum creatinine levels exceeding 160 μmol l-1 at 6 months had more severe lipid disorders already before transplantation.
Serum creatinine at 6 months was influenced by the number of acute rejection episodes ( P = 0.0001) and the age of the donor ( P = 0.009) while the number of acute rejections was found to be related to pretrans plant total cholesterol levels ( P = 0.0086) and the age of the recipient ( P = 0.025).
In conclusion, pretransplant lipoprotein disturbances have an impact on the early outcome of renal transplantation. Since there is a progresssion of hyperlipidaemia following transplantation, this may have an influence also on the cardiovascular morbidity and late graft dysfunction. 相似文献
Serum creatinine at 6 months was influenced by the number of acute rejection episodes ( P = 0.0001) and the age of the donor ( P = 0.009) while the number of acute rejections was found to be related to pretrans plant total cholesterol levels ( P = 0.0086) and the age of the recipient ( P = 0.025).
In conclusion, pretransplant lipoprotein disturbances have an impact on the early outcome of renal transplantation. Since there is a progresssion of hyperlipidaemia following transplantation, this may have an influence also on the cardiovascular morbidity and late graft dysfunction. 相似文献
10.
目的:探讨心脏克隆钾离子通道Kv1.4的C型失活(Kv1.4△N)在非洲蟾蜍卵母细胞上表达后的动力学特性以及酸中毒时的改变。方法:将Kv1.4△N cRNA(最大体积为50 n1)注入非洲爪蟾的卵母细胞内,于18℃孵育16h以上。电极采用两步法拉制,微电极由1.5 mm口径的电极拉制。电极内充3M KCl,电阻0.5~1.0MΩ。采用双微电极电压钳制法(two electrode voltage clamp,TEV)在室温下(20~24℃)记录电流。结果:与正常pH时相比,酸性环境下,Kv1.4△N的峰电流减小,在pH7.4时通道在去极化至-40mv激活,而pH 6.8时为-30mv激活;通道在pH 7.4时最大失活为0.384±0.072,而在pH6.8时为0.197±0.013;在pH6.8时通道复活减慢(P<0.05)。结论:酸中毒导致通道电流减小,并且使通道失活加快和恢复减慢。 相似文献