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T A Benke J W Clark P J Wisoff S Schneider C Balasubramaniam H K Hawkins J Laurent L Perling A Shehab 《Lasers in surgery and medicine》1989,9(6):602-615
Conventional suture repair of peripheral nerves results in a fibrotic reaction that is detrimental to nerve regeneration. As an alternative procedure known as "laser-assisted" repair, a laser can be used, along with a reduced number of sutures, to reanastomose served peripheral nerves. To explore the long-term implications of this technique, the right sciatic nerves of Sprague-Dawley rats were surgically cut and reanastomosed either by means of four epineurial sutures or two epineurial sutures and CO2 laser welds. Tensile strength, electrophysiology, histology, and functional studies were performed up to 11 months postoperatively. Tensile strength measurements indicate no long-term disadvantage with the laser-assisted technique, although the short-term tensile strength is lower than with conventional suture repair. The conduction velocities of the repaired nerves were similar for both techniques; however, laser-assisted repaired nerves were found to have lower stimulation thresholds and reduced branching compared to the suture repaired nerves. The measured functional recovery was similar for both repair techniques. 相似文献
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Vikram Sharma Kevin Chen Shehab A.R. Alansari Beni Verma Edward G. Soltesz Douglas R. Johnston Michael Zhen-Yu Tong Eric E. Roselli Per Wierup Gösta B. Pettersson A. Marc Gillinov Stephen G. Ellis Conrad Simpfendorfer Eugene H. Blackstone Samir Kapadia Lars G. Svensson Faisal G. Bakaeen 《The Annals of thoracic surgery》2021,111(5):1494-1501
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An enzyme-linked immunosorbent assay (ELISA) for antibody to mumps virus is described. It is standardized with sera from putatively susceptible and immune populations. The ranges for 49 susceptible and 19 immune children were an optical density of -0.020 to 0.028 and 0.133 to 0.640, respectively. When compared with the microneutralization assay for evaluating vaccine responses, the ELISA appeared to be more sensitive while retaining its specificity. The failure rate after mumps vaccination in 301 children was 1.7% (5 of 301) by the ELISA and 12% (36 of 301) or 7.6% (23 of 301) by the microneutralization assay, depending on whether a high (30-100 50% tissue culture infectious dose [TCID50]) or low (10-20 TCID50) dose of virus was used. 相似文献
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Enzyme-linked immunosorbent assay for susceptibility to varicella 总被引:13,自引:0,他引:13
Nonspecific reactions to the membrane antigen of varicella-zoster virus were found by fluorescent antibody assay in the sera of three children susceptible to the virus; one of these children subsequently developed varicella. None of the three sera, however, contained antibody to varicella-zoster virus that was detectable by enzyme-linked immunosorbent assay (ELISA). Moreover, the results of the ELISA clearly distinguished immune from susceptible individuals. The negative range was established with sera obtained from 15 individuals prior to the onset of varicella, and the positive range was established with sera obtained from 75 healthy donors with a history of varicella. The results of the ELISA correlated well with those of the fluorescent antibody assay (r = +0.84). Heterologous reactions to herpes simplex virus were found in one of seven sera tested, and such reactions to cytomegalovirus were found in two of five sera. Heterologous reactions to Epstein-Barr virus were found in none of eight sera tested. ELISA is easy, sensitive, and reproducible and can be used for routine screening for susceptibility to varicella. 相似文献
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Abdulla Shehab Asim A. Elnour Adel Sadik Mahmoud Abu Mandil Ali AlShamsi Aesha Al Suwaidi AkshayaSrikanth Bhagavathula Pinar Erkekoglu Farah Hamad Saif K. Al Nuaimi 《Saudi medical journal》2015,36(11):1290-1298