全文获取类型
收费全文 | 1310篇 |
免费 | 85篇 |
国内免费 | 8篇 |
专业分类
耳鼻咽喉 | 13篇 |
儿科学 | 32篇 |
妇产科学 | 31篇 |
基础医学 | 205篇 |
口腔科学 | 45篇 |
临床医学 | 85篇 |
内科学 | 240篇 |
皮肤病学 | 38篇 |
神经病学 | 62篇 |
特种医学 | 45篇 |
外科学 | 106篇 |
综合类 | 66篇 |
预防医学 | 114篇 |
眼科学 | 15篇 |
药学 | 165篇 |
中国医学 | 22篇 |
肿瘤学 | 119篇 |
出版年
2022年 | 14篇 |
2021年 | 22篇 |
2020年 | 15篇 |
2019年 | 21篇 |
2018年 | 17篇 |
2017年 | 17篇 |
2016年 | 26篇 |
2015年 | 22篇 |
2014年 | 33篇 |
2013年 | 53篇 |
2012年 | 84篇 |
2011年 | 89篇 |
2010年 | 79篇 |
2009年 | 52篇 |
2008年 | 80篇 |
2007年 | 78篇 |
2006年 | 61篇 |
2005年 | 65篇 |
2004年 | 74篇 |
2003年 | 56篇 |
2002年 | 43篇 |
2001年 | 32篇 |
2000年 | 29篇 |
1999年 | 36篇 |
1998年 | 8篇 |
1996年 | 9篇 |
1995年 | 10篇 |
1994年 | 5篇 |
1993年 | 7篇 |
1992年 | 19篇 |
1991年 | 14篇 |
1990年 | 13篇 |
1989年 | 24篇 |
1988年 | 22篇 |
1987年 | 18篇 |
1986年 | 23篇 |
1985年 | 10篇 |
1984年 | 19篇 |
1983年 | 16篇 |
1982年 | 5篇 |
1981年 | 7篇 |
1980年 | 11篇 |
1979年 | 15篇 |
1978年 | 6篇 |
1977年 | 5篇 |
1976年 | 5篇 |
1975年 | 5篇 |
1974年 | 4篇 |
1972年 | 4篇 |
1968年 | 3篇 |
排序方式: 共有1403条查询结果,搜索用时 15 毫秒
1.
P Aggarwal S K Sharma A B Dey T K Chattopadhyay M Mathur 《Postgraduate medical journal》1989,65(770):929-931
A case of malignant fibrous histiocytoma of the mediastinum presenting with unusual features of fever and leucocytosis is reported. This is the youngest patient reported in the literature who had this tumour in the mediastinum. 相似文献
2.
3.
4.
S K Chaudhuri R N Chattopadhyay S K Maitra S Ray S Chaudhuri 《Indian journal of physiology and pharmacology》1992,36(4):255-258
Effects of progesterone on four neurotransmitters (viz, noradrenaline, 5-HT, dopamine and histamine) of brain were seen in rats with intact ovaries. It was found that progesterone lowers the noradrenaline concentration in medulla, pons, midbrain, hypothalamus, thalami and pituitary, uniformly, when the rats were killed within 4 hours of progesterone injection. At longer intervals (48 hrs) effects of progesterone were seen when progesterone in heavy dose was administered to rats pretreated with estrogen. It is likely that one of the modes of action of the oral contraceptives may be the reduction of noradrenaline content in selected areas of brain, by progesterone. It is also suggested, therapeutic usage of progesterone carries the risk of development of depression in the user. 相似文献
5.
In vivo gene therapy for pyridoxine-induced neuropathy by herpes simplex virus-mediated gene transfer of neurotrophin-3. 总被引:6,自引:0,他引:6
Munmun Chattopadhyay Darren Wolfe Shaohua Huang James Goss Joseph C Glorioso Marina Mata David J Fink 《Annals of neurology》2002,51(1):19-27
Neurotrophic factors have been demonstrated to prevent the development of peripheral neuropathy in animal models, but the therapeutic use of these factors in human disease has been limited by the short serum half-life and dose-limiting side effects of these potent peptides. We used peripheral subcutaneous inoculation with a replication-incompetent, genomic herpes simplex virus-based vector containing the coding sequence for neurotrophin-3 to transduce sensory neurons of the rat dorsal root ganglion in vivo, and found that expression of neurotrophin-3 from the vector protected peripheral sensory axons from neuropathy induced by intoxication with pyridoxine assessed by electrophysiological (foot sensory response amplitude, and conduction velocity, and H-wave), histological (nerve morphology and morphometry), and behavioral measures of proprioceptive function. In vivo gene transfer using herpes simplex virus vectors provides a unique option for treatment of diseases of the sensory peripheral nervous system. 相似文献
6.
Scrub typhus vaccine candidate Kp r56 induces humoral and cellular immune responses in cynomolgus monkeys
下载免费PDF全文
![点击此处可从《Infection and immunity》网站下载免费的PDF全文](/ch/ext_images/free.gif)
Chattopadhyay S Jiang J Chan TC Manetz TS Chao CC Ching WM Richards AL 《Infection and immunity》2005,73(8):5039-5047
A truncated recombinant 56-kDa outer membrane protein of the Karp strain of Orientia tsutsugamushi (Kp r56) was evaluated in cynomolgus monkeys (Macaca fascicularis) for immunogenicity and safety as a vaccine candidate for the prevention of scrub typhus. This recombinant antigen induced strong humoral and cellular immune responses in two monkeys and was found to be well tolerated. Antigen-specific immunoglobulin M (IgM) and IgG were produced to almost maximal levels within 1 week of a single immunization. Peripheral blood mononuclear cells from vaccinated animals showed an induction of antigen-specific proliferation and gamma interferon production. The Kp r56 was not as efficient as infection with live organisms in preventing reinfection but was able to reduce the inflammation produced at the site of challenge. This report describes the results of the first systematic study of the immunogenicity of a recombinant scrub typhus vaccine candidate in a nonhuman primate model. 相似文献
7.
Regulated expression of the IL-31 receptor in bronchial and alveolar epithelial cells, pulmonary fibroblasts, and pulmonary macrophages. 总被引:1,自引:0,他引:1
Randeep S Jawa Souvik Chattopadhyay Erin Tracy Yanping Wang Kristin Huntoon Merril T Dayton Heinz Baumann 《Journal of interferon & cytokine research》2008,28(4):207-219
Interleukin-31 (IL-31), an IL-6 cytokine family member, is proposed to play a role in animal models of airway hyperreactivity. It is produced by activated T cells and signals via a heterodimeric receptor complex composed of IL-31Ralpha and OSMRbeta. Only low levels of IL-31Ralpha expression have been demonstrated in pulmonary epithelial cell lines, however, and little is known about the ability to regulate its expression and signaling. Therefore, primary cultures of human bronchial and alveolar epithelial cells, pulmonary fibroblasts, pulmonary macrophages, and established lines of immortalized bronchial epithelial cells (HBE) and alveolar carcinoma cells (A549) were analyzed by RT-PCR, immunoblotting, and thymidine incorporation. Distinct, cell type-specific regulation of IL-31Ralpha expression was detected. Transforming growth factor-beta (TGF-beta) enhanced IL-31Ralpha mRNA expression in primary cultures and established lines of epithelial cells, but not in macrophages. In contrast, interferon-gamma (IFN-gamma) induced IL-31Ralpha mRNA expression in macrophages. IL-31Ralpha protein expression was below detection threshold in primary epithelial cell cultures but was detectable in A549 cells and increased with TGF-beta treatment. In HBE and A549 cells, TGF-beta pretreatment increased IL-31-mediated Stat3 and extracellular signal-regulated kinase 1/2 (ERK1/2) phosphorylation. In A549 cells, TGF-beta magnified IL-31-dependent suppression of proliferation. The data suggest that increased IL-31Ralpha expression correlates with an enhanced response to IL-31. 相似文献
8.
The objective of these studies was to examine the mechanism by which the specific activity of heart sarcolemma 5'-nucleotidase decreases as function of age. We examined the kinetic properties and the lipid composition of the sarcolemma from animals with different ages. The age groups used were 1 month, 6-8 months and 13-15 months. It was found that the Km of this enzyme increases as the animal develops from 1 month to 6-15 months. The opposite was true with 5'-nucleotidase Vmax. There was no significant difference between the middle age and the older age groups in those parameters. The results of these experiments suggest that the increase in Km in sarcolemma 5'-nucleotidase could be due to the reduction of the sarcolemmal polyunsaturated fatty acid concentration, the only lipid alteration observed. 相似文献
9.
Chattopadhyay P Pakstis AJ Mukherjee N Iyengar S Odunsi A Okonofua F Bonne-Tamir B Speed W Kidd JR Kidd KK 《European journal of human genetics : EJHG》2003,11(10):760-769
We have constructed haplotypes based on normal variation at six polymorphic sites-five single nucleotide polymorphisms (SNPs) and one short tandem repeat polymorphism (STRP)-at the RET locus for samples of normal individuals from 32 populations distributed across the major continental regions of the world. The haplotyped system spans 41.6 kilobases and encompasses most of the coding region of the gene. All of the markers are polymorphic in all regions of the world and in most individual populations. Expected heterozygosities for the six-site haplotypes range from 82 to 94% for all populations studied except for two Amerindian groups from the Amazon basin at 61 and 76%. Individual populations had from four to eight haplotypes with frequencies exceeding 5%. In general, African, southwest Asian and European groups have the highest numbers of total and of commonly occurring haplotypes; the lowest numbers are observed in Amerindian populations. Overall linkage disequilibrium (LD) for the five SNP sites was very significant (P=0.001) for all the non-African populations, but significant at that level for only one of the seven African populations. In general, the permutation-based xi coefficient that quantifies overall LD tends to increase the farther the population is from Africa, but variability of this measure of LD is often large within geographic regions. Pairwise LD measures among the SNPs also show considerable variation among populations. Association of STRP alleles with the SNP-defined background haplotypes is generally higher outside of Africa than in Africa, but is highly variable. 相似文献
10.
Transfer RNAs were isolated from phage e4-infected Vibrio eltor Mak 757 cells. These were aminoacylated with 14 individual 3H-labeled L-amino acids. Hybridization of these [3H]aminoacyl-tRNAs with phage e4 DNA revealed that the phage e4 encodes tRNAs for arginine, tryptophan, tyrosine, leucine, and isoleucine. Direct aminoacylation of phage-coded tRNA molecules isolated from phage DNA-RNA hybrids also confirmed this observation. 相似文献