Amplatzer封堵器经导管治疗动脉导管未闭20例

目的　应用 Amplatzer封堵器经导管治疗动脉导管未闭并对其即刻和近期疗效进行评价 .方法　全组 2 0例 ,年龄 2 0± 17( 0 .8～ 5 1)岁 ,体质量 37± 2 0 ( 6～ 70 ) kg,动脉导管未闭最窄处直径为 5 .1± 1.8( 2 .9～ 10 .0 ) mm.经 6或 7F导管置入 Amplatzer封堵器 ,术后 10 min行侧位降主动脉造影 ,术后 1d及 1mo行超声心动图检查观察有无残余分流及动脉导管未闭再通 .结果　全组技术成功率为 10 0 % ,术后 10 min降主动脉造影示 ,6例有微 -少量残余分流 ,术后 2 4h,术后 1mo超声心动图未见残余分流及再通 .结论　应用 Amplatzer封堵器治疗直径达 10 mm的动脉导管未闭是一种安全有效的介入方法 ,操作简便 ,成功率高 ,近期疗效可靠 ,中远期疗效尚待进一步观察     

RP-HPLC进行甲氨蝶呤血药浓度监测时保留时间校准方法研究

目的 建立RP—HPLC进行MTX血药浓度监测时保留时间校准方法。方法应用容量因子与流动相比例的三次方多项式模型，建立MTX流动相比例与保留时间的函数，并通过此函数用保留时间求算流动相比例，与理论流动相比例比较，从而计算流动相应该补充组分的量，达到调整保留时间的目的。结果四种不同色谱条件下容量因子与流动相比例的三次方多项式模型拟合关系良好，R0均为1．000，保留时间经校准后RSD为0.9％(n=5)。结论本方法能准确校准因流动相比例引起变化的保留时间，适用于MTX血药浓度监测时保留时间的校准，方法新颖、有效、简单。     

系膜细胞来源的白细胞介素-13对系膜细胞细胞因子基因表达的研究(英文)

目的　白细胞介素 1 3(IL 1 3)是新近发现的一种抗炎性细胞因子 ,其在肾小球肾炎中的作用尚不清楚 ,该研究探讨脂多糖 (LPS)对体外培养的人肾小球系膜细胞 (HMC)表达IL 1 3作用以及IL 1 3对HMC促炎性细胞因子、趋化因子和促纤维化因子基因表达的影响。方法　体外培养HMC ,加入不同浓度的LPS和 (或 )IL 1 3后 ,用逆转录 -聚合酶链反应和ELISA检测HMCIL 1 3mRNA表达和细胞培养上清液中IL 1 3蛋白含量 ;应用核酸酶保护法检测HMC肿瘤坏死因子 α(TNF α)、白介素 - 1α(IL 1α)、白介素 - 1 β(IL 1 β)、单核细胞趋化蛋白 1(MCP 1 )、白介素 8(IL 8)、转化生长因子 - β1 (TGF β1 )mRNA的表达。 结果　未予LPS刺激的HMC不表达IL 1 3mRNA和蛋白 ;LPS呈剂量依赖性和时间依赖性诱导HMC表达IL 1 3mRNA和分泌IL 1 3蛋白。HMC受LPS刺激后 1 2h即可表达IL 1 3mRNA ,4 8h达高峰 ,72h仍维持在较高的水平。HMC受LPS刺激后 2 4h ,其培养上清液中检测到IL 1 3蛋白 ,4 8h和 72h进一步增加。外源性IL 1 3呈剂量依赖性地抑制LPS诱导的系膜细胞TNF α ,IL 1α ,IL 1 β ,MCP 1 ,IL 8,TGF β1mRNA的表达。应用抗IL 1 3抗体中和内源性IL 1 3后 ,上述炎症因子表达增强。结论　IL 1 3是HMC自分泌因子。IL 1 3可抑制LPS诱导     

两种免疫调节剂增强弓形虫W2b2a表位疫苗的小鼠保护作用

目的:探讨匹多莫德、沙利度胺增强弓形虫W2b2a表位疫苗刺激机体产生免疫应答和保护免疫的效果。方法:将匹多莫德、沙利度胺分别与弓形虫W2b2a表位疫苗混合肌注免疫小鼠,检测其细胞免疫和体液免疫,并观察其受到弓形虫攻击感染后的生存时间。结果:pc DNA3-W2b2a加匹多莫德组、pc DNA3-W2b2a加弗氏佐剂组、pc DNA3-W2b2a加沙利度胺组小鼠血清IFN-γ水平显著高于pc DNA3-W2b2a组(P0.05);pc DNA3-W2b2a加匹多莫德组、pc DNA3-W2b2a加弗氏佐剂组Ig G抗体水平、CD4+/CD8+比值、T细胞增殖活性显著高于pc DNA3-W2b2a组和pc DNA3-W2b2a加沙利度胺组(P0.05);小鼠攻击试验表明,免疫组小鼠存活时间明显长于对照组(P0.05)。结论:匹多莫德、沙利度胺可增强弓形虫W2b2a表位疫苗免疫小鼠的保护作用。     

MR diffusion-weighed imaging of rabbit liver

AIM: To study the techniques of MR diffusion-weighed imaging (DWI) for normal rabbit liver. METHODS: After 15 normal New Zealand white rabbits and one New Zealand white rabbit implanted with VX-2 tumor were anesthetized with 3% soluble pentobarbitone, DWI was performed respectively for different b values, repetition times (TR) or thicknesses, when other parameters were the same and magnetic resonance imaging (MRI) was performed respectively, or with different field of views (FOV) or coil when other parameters were the same. The distinction between groups was analyzed by SPSS10.0 with apparent diffusion coefficient (ADC), quality index (QI) or signal-noise ratio (SNR). RESULTS: As b value increased, liver ADC, QI and SNR of DWI became smaller and simultaneously (F=292.87, 156.1, 88.23, P<0.01). QI of DWI was high, when b value was 10, 50 or 100 respectively, but the distinction between them was insignificant; when b value was 800, QI and SNR of DWI were low. QI and SNR of DWI had no significant difference between TR=4000, 6000 and 8000. QI of DWI with 2 mm thickness was bigger than that with 5 mm thickness (t=3.04, P<0.01), but SNR of DWI with 2 mm thickness was significantly smaller (t=-17.86, P<0.01). SNR of MRI with knee joint coil was obviously bigger than that with cranium coil [t=-5.77 (T1WI) or -4.02 (T2WI), P<0.01], but QI of MRI was smaller on the contrary [t=7.10 (T1WI) or 3.97 (T2WI), P<0.01]. When FOV was enlarged gradually, SNR of MRI increased [F=85.81 (T1WI) or 221.96 (T2WI), P<0.01], but QI firstly increased, then decreased [F=68.67 (T1WI) or 69.46 (T2WI), P<0.01] and QI of MRI was the biggest when FOV was 20 cm脳15 cm. CONCLUSION: The scanning technique is very important in DWI of rabbit iiver and the overall quality of DWI with b (100 s/mm~2), thickness (2 mm), cranium coils and FOV (20 cm×15 cm) was best in our study, when other parameters were the same.     

An improved method for the isolation and culture of rat epidermal stem cells

The management of burns and injuries using novel treatment strategies involving epidermal stem cells (ESC) requires a better understanding of the biology of these cells, in particular, their isolation and the maintenance of their unique characteristics in culture. The purpose of this study was to describe an improved method for isolating putative ESC from fetal rat skin and to maintain them long term in culture. Single ESC suspensions were obtained from fetal rat skin by enzyme digestion containing 0.5% neutral protease. The target cells were harvested by rapid adherence on type IV collagen plates and were cultured in complex DMEM. After primary isolation, cells were continuously cultured in K-serum free medium. After reaching 70-80% confluence, the cells were digested with 0.25% trypsin at 37°C for 5-10 minutes, and passaged at a ratio of 1:2. The cultured ESC showed good growth, resulting in cell viability of over 98%. Four days later, clones containing 100-200 cells were detected, showing cobblestone-like characteristics. The rapidly adherent cells were positive for keratin 15, 19 and P63. Eighty three percent of cells expressed β1 integrin. The growth-curve showed that the rapidly adherent cells were in the exponential growth phase. The protocol described in this paper provides a simplified and effective method to isolate and maintain long-term culture of epidermal stem cells from fetal rat skin. This method should be valuable for isolating and studying ESC from various transgenic rat lines that are currently available.     

Structure and bioactivity of triterpenoids from the stems of Schisandra sphenanthera

Two new triterpenoids, schisphendilactone A and B (1 and 2), together with three known triterpenoids, were isolated from the stems of Schisandra sphenanthera. Their structures were elucidated by spectroscopic methods, and the absolute configuration of 1 was determined by single-crystal X-ray diffraction. Compound 2 showed moderate inhibitory activity against SW480 cancer cell line, and compound 5 exhibited promising anti-HIV-1 activity with EC₅₀ value of 0.52 μg ml^?1 and therapeutic index value of 117.12.     

二甲双胍治疗多囊卵巢综合征克罗米酚抵抗疗效观察

目的: 探讨二甲双胍(MTF)治疗多囊卵巢综合征(PCOS)不孕患者克罗米酚(CC)抵抗的效果. 方法: 22例CC抵抗PCOS不孕患者中合并胰岛素抵抗(IR)者20例,改为口服MTF,3 mo未妊娠者加服CC 3个周期. 采取自身对照法,观察其用药前后排卵恢复情况、妊娠结局及血胰岛素水平、IR指数、体重指数(BMI)、性激素等指标的变化. 结果: 20例合并IR的患者服用MTF 6 mo后12例(60%)恢复排卵;其中10例(50%)妊娠,均未发生流产;8例无效,但用药6 mo后BMI、空腹胰岛素(I0),IR指数,T及LH/FSH均明显降低(P均＜0.05). 结论: CC抵抗PCOS不孕患者多数存在IR,应用MTF治疗可改善患者的排卵率及妊娠率,且经济方便.     

杭州市青少年自杀问题现况调查

目的:了解青少年自杀问题的现况与相关因素。方法:采用分层整群随机抽样的方法,抽取7335例在校学生,包括初中生2695名、高中生3937名、大学生703名。采用2004年中国疾病预防控制中心编制的《中国青少年健康相关行为调查问卷》,对人口学资料、最近12个月内的自杀问题及相关因素进行调查。结果:本研究样本的自杀意念的检出率为14.3%,自杀计划的检出率为6.9%,自杀行为的检出率为2.1%,多次自杀行为的检出率为1.0%。自杀问题的相关因素分析发现:年级越低(OR=0.92)、求助行为越少(OR=0.62)、母亲受教育程度越高(OR=1.15)、孤独感越多(OR=1.41)、自我伤害行为越多(OR=1.40),自杀意念的检出率越高(均P0.05);求助行为越少(OR=0.67)、孤独感越多(OR=1.21)、自我伤害行为越多(OR=1.49),自杀计划的检出率越高(均P0.05);不安全感(OR=1.16)、失眠(OR=1.33)、抑郁情绪(OR=2.30)、自我伤害行为(OR=2.45)越多,自杀行为的检出率越高(均P0.05);年级越高(OR=1.28),抑郁情绪(OR=3.78)、自我伤害行为越多(OR=2.53),多次自杀行为的检出率越高(均P0.05)。结论:本研究显示杭州市青少年自杀问题的检出率较高,青少年自杀问题可能与孤独、不安全感、抑郁等负面情绪以及来自学校和家庭的压力和缺少社会支持等有关。     

痴呆患者血清和脑脊液中炎前和抗炎细胞因子的变化

目的:研究炎前和抗炎细胞因子在不同痴呆患者血清和脑脊液中的变化并探讨其临床意义. 方法:2003-09/2005-08我院和台山市人民医院神经科就诊的70例老年痴呆患者作为观察对象,其中阿尔茨海默病(AD)30例、混合性痴呆(MD)15例、血管性痴呆(VD)25例. 用夹心式酶联免疫吸附法测定各组老年人血清和脑脊液中白介素-1β(IL-1β),白介素-6(IL-6),肿瘤坏死因子α(TNFα)和白介素1受体拮抗剂(IL-1ra)的水平. 结果:各痴呆组患者脑脊液中TNFα的水平较正常老年人有不同程度升高,而IL-1ra则不同程度降低(P＜0.05或P＜0.01);AD组患者脑脊液中IL-1β水平高于对照组(P＜0.05),IL-6含量较对照组却无增高;VD组患者脑脊液中IL-6水平虽显著高于对照组(P＜0.05),但IL-1β含量与对照组相比无增高; MD组患者IL-1β,IL-6水平较对照组均有增高(P＜0.01). 各痴呆组血清中IL-1β,IL-6,TNFα和IL-1ra的水平与正常老年人相比无差异. 相关分析显示不同痴呆患者血清与其相对应的脑脊液中炎前和抗炎细胞因子水平之间无相关. 结论:痴呆患者脑内炎前和抗炎细胞因子的变化可能是其神经病理损害因素.