全文获取类型
收费全文 | 977篇 |
免费 | 65篇 |
国内免费 | 32篇 |
专业分类
耳鼻咽喉 | 5篇 |
儿科学 | 53篇 |
妇产科学 | 10篇 |
基础医学 | 144篇 |
口腔科学 | 13篇 |
临床医学 | 88篇 |
内科学 | 210篇 |
皮肤病学 | 20篇 |
神经病学 | 38篇 |
特种医学 | 63篇 |
外科学 | 161篇 |
综合类 | 59篇 |
预防医学 | 35篇 |
眼科学 | 10篇 |
药学 | 55篇 |
肿瘤学 | 110篇 |
出版年
2022年 | 10篇 |
2021年 | 20篇 |
2020年 | 9篇 |
2019年 | 5篇 |
2018年 | 16篇 |
2017年 | 9篇 |
2016年 | 17篇 |
2015年 | 24篇 |
2014年 | 28篇 |
2013年 | 32篇 |
2012年 | 26篇 |
2011年 | 40篇 |
2010年 | 38篇 |
2009年 | 30篇 |
2008年 | 37篇 |
2007年 | 41篇 |
2006年 | 45篇 |
2005年 | 38篇 |
2004年 | 36篇 |
2003年 | 35篇 |
2002年 | 35篇 |
2001年 | 32篇 |
2000年 | 33篇 |
1999年 | 31篇 |
1998年 | 41篇 |
1997年 | 42篇 |
1996年 | 38篇 |
1995年 | 25篇 |
1994年 | 18篇 |
1993年 | 23篇 |
1992年 | 17篇 |
1991年 | 9篇 |
1990年 | 13篇 |
1989年 | 19篇 |
1988年 | 14篇 |
1987年 | 20篇 |
1986年 | 16篇 |
1985年 | 20篇 |
1984年 | 5篇 |
1983年 | 9篇 |
1982年 | 8篇 |
1981年 | 6篇 |
1980年 | 4篇 |
1979年 | 6篇 |
1978年 | 4篇 |
1977年 | 6篇 |
1970年 | 3篇 |
1969年 | 6篇 |
1968年 | 16篇 |
1967年 | 9篇 |
排序方式: 共有1074条查询结果,搜索用时 15 毫秒
1.
2.
3.
A. Giacometti O. Cirioni G. Greganti A. Fineo R. Ghiselli M. Del Prete F. Mocchegiani B. Fileni F. Caselli E. Petrelli V. Saba G. Scalise 《European journal of clinical microbiology & infectious diseases》2002,21(7):553-556
The in vitro activities of povidone iodine, potassium peroxymonosulfate, and dimethyldidecylammonium chloride were investigated
against 379 nosocomial isolates of Staphylococcus aureus and Pseudomonas aeruginosa responsible for surgical wound infections in patients operated on between July 1995 and June 2001. Overall, the isolates
were inhibited by the antiseptics at concentrations below those used routinely. In spite of increasing resistance to the various
antibiotics used to treat surgical wound infections, no significant variation in the susceptibility to antiseptics was demonstrated
during this 6-year study.
Electronic Publication 相似文献
4.
G. Miragliotta R. Del Prete R. Sabato A. Cassano N. Carnimeo 《European journal of epidemiology》1992,8(5):748-749
In October 1990 pneumonia due toLegionella pneumophila was diagnosed in two employees working in the area of Apulia, southern Italy, where artesian wells were in construction. Although the exposure to excavation has been associated with Legionnaires' disease, in our investigation the illness occurred only in those employees who were present when the water emerged from the ground under high pressure. On the basis of this report, water appears as the most likely reservoir of the organism and the main route of infection.Corresponding author. 相似文献
5.
6.
7.
Relative carriage rates of nuclear dehydrogenating clostridia in two populations of different colorectal cancer risk
下载免费PDF全文
![点击此处可从《Journal of clinical pathology》网站下载免费的PDF全文](/ch/ext_images/free.gif)
Carriage of nuclear dehydrogenating clostridia has been associated with colon cancer and implicated in its aetiology. This study has compared the carriage of these organisms in a British population at high risk for the development of colon cancer with a low risk Nigerian population. Clostridia were found in all of the stools from both populations. Nuclear dehydrogenating clostridia were only found in the stools of the British subjects (32%). These results support the suggestion that the carriage rate of nuclear dehydrogenating clostridia in a population is related to the risk of colon cancer. 相似文献
8.
Development of hatching blastocysts from immature human oocytes following in-vitro maturation and fertilization using a co-culture system 总被引:8,自引:0,他引:8
Hwu YM; Lee RK; Chen CP; Su JT; Chen YW; Lin SP 《Human reproduction (Oxford, England)》1998,13(7):1916-1921
Recently, in-vitro maturation (IVM) of immature human oocytes recovered
from non-stimulated follicles has been applied in the treatment of
infertility. However, in previous reports, very few embryos cultured in
conventional medium have reached the expanded blastocyst stage following
in-vitro maturation and fertilization (IVM/IVF). The objective of this
study was to investigate whether the developmental competence of human
embryos following IVM/IVF could be enhanced by the use of a human ampullary
cell co-culture system. Immature human oocytes were aspirated from small
follicles at Caesarean section and then cultured in medium containing human
menopausal gonadotrophin for 36 to 48 h, followed by insemination. Zygotes
were randomly cultured either in conventional culture medium alone or in
the co-culture system. Of 48 embryos cultured in conventional medium alone,
all arrested at the 2-16- cell stage on day 3 after insemination. Of 46
embryos cultured in the co-culture system, 26 embryos (56.5%) arrested at
the 2-16-cell stage. Six embryos (13%) developed to the morula stage.
Fourteen embryos (30.4%) developed to expanded blastocysts and two
blastocysts were hatching on day 7 after insemination. We conclude that
co-culture significantly enhances the development of blastocysts in embryos
resulting from IVM/IVF.
相似文献
9.
In vitro production of IgE by human peripheral blood mononuclear cells. I. Rate of IgE biosynthesis
下载免费PDF全文
![点击此处可从《Clinical and experimental immunology》网站下载免费的PDF全文](/ch/ext_images/free.gif)
S. Romagnani E. Maggi G. F. Del Prete R. Troncone M. Ricci 《Clinical and experimental immunology》1980,42(1):167-174
IgE protein and grass-specific IgE antibodies were detected in the supernatants of 7-day cultures of unstimulated and pokeweed mitogen (PWM) stimulated human blood mononuclear cells from non-atopic and grass pollen-sensitive individuals. Significant amounts of IgE protein were detected in culture supernatants of grass-sensitive individuals and, even at lower levels, in those of non-atopic subjects. In contrast, detectable amounts of grass-specific antibodies were found only in the culture supernatants of grass-sensitive subjects. The mean values of total and grass-specific IgE detected in the supernatants of unstimulated and PWM-stimulated cultures did not differ statistically. Time sequence studies showed that IgE concentrations, measured in the 7-day supernatants, were due to a continuous release from the cells of IgE quantities progressively decreasing up to days 7 or 8. Comparison of the IgE protein and IgE antibody found in the 7-day culture supernatants to those released from initial cell pellets by treatment with acid buffer or freezing and thawing, showed that the IgE detected in 7-day supernatants could result, in part, from the release of cytophilic IgE bound to basophil or other cell types and in part also from the release of preformed lymphocyte cytoplasmic IgE into the supernatant fluids during the course of culture. In most non-atopic subjects and in some grass-sensitive patients the preformed IgE accounted virtually for the total IgE detected in the 7-day culture supernatants. However, the increase of IgE above the levels measured in the initial cell pellets, which was found in most grass-sensitive subjects, clearly reflected newly synthesized IgE. Both cycloheximide and puromycin were capable of reducing significantly the IgE concentration in culture supernatants when it was greater than the amount found in the initial cell pellets. The treatment of cells with mitomycin C was also able to decrease significantly the amount of IgE released in the supernatant after day 3 of culture. 相似文献
10.
Direct induction of human B-cell differentiation by recombinant interleukin-2. 总被引:5,自引:0,他引:5
下载免费PDF全文
![点击此处可从《Immunology》网站下载免费的PDF全文](/ch/ext_images/free.gif)
S Romagnani G Del Prete M G Giudizi R Biagiotti F Almerigogna A Tiri A Alessi M Mazzetti M Ricci 《Immunology》1986,58(1):31-35
Recombinant interleukin-2 (rIL-2) induced highly purified human tonsillar B cells to differentiate into immunoglobulin (Ig)-producing cells in vitro. The B-cell response was not due to rIL-2-contaminating substances, but reflected the activity of IL-2 itself, since it was inhibited by addition to the cultures of anti-TAC monoclonal antibody. The rIL-2-induced B-cell response was apparently not mediated by factors released by residual T cells present in B-cell suspensions at undetectable levels, since supernatants (SN) from unstimulated autologous T cells cultured at concentrations even much higher than those possibly contaminating B-cell suspensions did not induce any detectable Ig production. In addition, the Ig production by B cells cultured with SN prepared from high numbers of autologous T cells stimulated with rIL-2, as well as from allo-activated or mitogen-stimulated T cells, was of the same magnitude as the Ig production resulting from direct addition of rIL-2 concentrations comparable with those present in the supernatants. After centrifugation on Percoll density gradients, most of the tonsillar B cells responsive to rIL-2 were recovered in the lower density cell fraction containing a number of larger activated B cells. Moreover, B-cell enriched suspensions from peripheral blood (PB) (which usually contains a lower number of in vivo activated B cells than tonsil) showed poor or no response to rIL-2 alone, but displayed significant Ig production when rIL-2 was added to the cultures in the presence of Staphylococcus aureus Cowan I (SAC) bacteria.(ABSTRACT TRUNCATED AT 250 WORDS) 相似文献