Experience of detection of rubella markers during local outbreaks in West Siberia

This paper presents an analysis of samples from 75 patients for the presence of rubella virus, viral RNA, and specific antibodies. For all samples, RNA detection was higher than virus isolation. It was found that it was not possible to isolate rubella virus in every sixth clinical sample containing the viral RNA. Primary structures of the site (from the 8072nd to 8291st nucleotide) of the rubella virus genome from 14 samples were determined. This paper shows that all the samples of rubella virus belong to the first genotype, subgroups 1h, and very likely to subgroups 1a and 1F. For the first time, the circulation of rubella virus of the genotype has been shown both prior to the start of mass vaccination in Western Siberia and after.     

HPLC in biopharmaceutical investigations of drugs representing pyrimidine derivatives (A review)

Pyrimidine derivatives are widely used for the treatment of viral diseases and cancer. The analysis of pyrimidine derivatives is typically performed using various chromatographic techniques, in particular, reversed-phase high performance liquid chromatography (HPLC). The separation is typically carried out with (7–30)-cm-long C₈ and C₁₈ silica gel columns, mainly at room temperature, and a 1–1.5 ml-min eluent flow rate. The column is eluted in an isocratic or gradient system, and a variety of mobile phases have been proposed. The detection is based on optical absorption or fluorescence measurements, or makes use of mass spectrometry. Various methods of extraction of pyrimidine derivatives from biological samples are discussed, and the corresponding detection limits are presented. __________ Translated from Khimiko-Farmatsevticheskii Zhurnal, Vol. 41, No. 5, pp. 26–34, May, 2007.     

Serum beta 2-microglobulin after five-day administration of cisplatin

Patients with testicular tumors receiving the Einhorn combination chemotherapy with cisplatinum administration for five consecutive days were evaluated for signs of glomerular filtration rate impairment by means of serum beta 2-microglobulin measurements before and on days 2 and 5 after cessation of cisplatin administration and then before repeated cycles of the same chemotherapeutic regimen. Though no significant changes in the level of serum beta 2-microglobulin could be found, an evident increase in the values was seen indicating that in more aggressive cisplatin administration schedules not only tubular impairment might contribute to possible nephrotoxic effects.     

Clinico-anatomical basis for using the variants of proximal gastric vagotomy in the treatment of gastroduodenal ulcers

Anatomical examinations (33 cadavers) for studying parasympathetic innervation of the upper abdominal organs formed the basis for developing a new variant of proximal gastric vagotomy--anterior seromyotomy of the body and fundus of the stomach in combination with posterior truncal or posterior selective vagotomy. This variant excludes mobilization of the esophagus and makes it possible to preserve the ligament apparatus of the esophago-gastric junction as well as the parasympathetic innervation of the pancreas, small and large intestine, kidneys, and adrenals, which may be a measure for the prevention of some ++post-vagotomy complications.     

Interaction between synthetic petides and individual components of the blood clotting system

Bulletin of Experimental Biology and Medicine -     

Cytokeratin expression in squamous-cell metaplasia of the cervix uteri: an immunohistochemical study using monoclonal antibodies]

Immunohistochemical study of the exo- and endocervix in squamous metaplasia is performed using EE21-06d monoclonal antibodies revealing simultaneously five cytokeratinous polypeptides characteristic of squamous epithelium, and f-12-19h revealing simultaneously wide spectrum of cytokeratinous polypeptides characteristic of the epithelium various types. PAP method and the reaction of immunofluorescence were used. Monoclonal antibodies 12-19d label cells of all layers of normal exocervix, endocervix and metaplastic squamous epithelium, both immature and differentiated. Monoclonal antibodies EE21-06d react in the exocervix with suprabasal and higher located cells and in the endocervix with reserve cells only. Positive reaction with EE21-06d in the metaplastic foci increases in proportion with the squamous cell differentiation, thus this antibody being the marker of reserve cells and the degree of the squamous metaplasia maturity.