全文获取类型
收费全文 | 1138篇 |
免费 | 80篇 |
国内免费 | 3篇 |
专业分类
耳鼻咽喉 | 2篇 |
儿科学 | 27篇 |
妇产科学 | 25篇 |
基础医学 | 170篇 |
口腔科学 | 21篇 |
临床医学 | 126篇 |
内科学 | 174篇 |
皮肤病学 | 38篇 |
神经病学 | 196篇 |
特种医学 | 34篇 |
外科学 | 134篇 |
综合类 | 4篇 |
预防医学 | 92篇 |
眼科学 | 11篇 |
药学 | 83篇 |
中国医学 | 6篇 |
肿瘤学 | 78篇 |
出版年
2023年 | 20篇 |
2022年 | 14篇 |
2021年 | 47篇 |
2020年 | 35篇 |
2019年 | 33篇 |
2018年 | 42篇 |
2017年 | 34篇 |
2016年 | 23篇 |
2015年 | 36篇 |
2014年 | 49篇 |
2013年 | 47篇 |
2012年 | 72篇 |
2011年 | 79篇 |
2010年 | 45篇 |
2009年 | 38篇 |
2008年 | 67篇 |
2007年 | 47篇 |
2006年 | 52篇 |
2005年 | 47篇 |
2004年 | 44篇 |
2003年 | 30篇 |
2002年 | 28篇 |
2001年 | 8篇 |
2000年 | 30篇 |
1999年 | 19篇 |
1998年 | 9篇 |
1997年 | 9篇 |
1996年 | 13篇 |
1995年 | 13篇 |
1994年 | 9篇 |
1993年 | 10篇 |
1992年 | 19篇 |
1991年 | 9篇 |
1990年 | 8篇 |
1989年 | 18篇 |
1988年 | 9篇 |
1987年 | 9篇 |
1986年 | 10篇 |
1985年 | 4篇 |
1984年 | 4篇 |
1983年 | 11篇 |
1979年 | 11篇 |
1978年 | 5篇 |
1977年 | 6篇 |
1976年 | 4篇 |
1974年 | 4篇 |
1973年 | 5篇 |
1972年 | 5篇 |
1915年 | 2篇 |
1914年 | 3篇 |
排序方式: 共有1221条查询结果,搜索用时 0 毫秒
1.
Maud Bessems Benedict M Doorschodt Arlène K van Vliet Thomas M van Gulik 《Liver transplantation》2005,11(5):539-546
For experimental machine perfusion (MP) of the liver, the modified University of Wisconsin solution (UW-G) is most often used. In our search for an enriched MP preservation solution, Polysol was developed. Polysol is enriched with various amino acids, vitamins, and other nutrients for the liver metabolism. The aim of this study was to compare Polysol with UW-G for MP preservation of the liver. Rat livers were preserved during 24 hours with hypothermic MP using UW-G (n = 5) or Polysol (n = 5). Hepatocellular damage (aspartate aminotransferase [AST], alanine aminotransferase [ALT], lactate dehydrogenase [LDH], alpha-glutathione-S-transferase [alpha-GST]) and bile production were measured during 60 minutes of reperfusion (37 degrees C) with Krebs-Henseleit buffer. Control livers were reperfused after 24 hours of cold storage in UW (n = 5). MP using UW-G or Polysol showed less liver damage when compared with controls. Livers machine perfused with Polysol showed less enzyme release when compared to UW-G. Bile production was higher after MP using either UW-G or Polysol compared with controls. In conclusion, machine perfusion using Polysol results in better quality liver preservation than cold storage with UW and machine perfusion using UW-G. 相似文献
2.
Minerva Giménez y Ribotta Christelle Roudet Françoise Sandillon Alain Privat 《Brain research》1996,707(2):245
The synaptic connections established by grafted noradrenergic (NA) neurons into the lesioned adult rat spinal cord were analysed using immunocytochemistry at the electron microscopic level. An embryonic cell suspension of the locus coeruleus region from E-13 rat embryos was transplanted into the spinal cord following either: (1) spinal cord transection or (2), partial selective denervation by 6-hydroxy dopamine (6-OH DA). One month after grafting, the NA-neurons established, in the two models, an innervation pattern similar to that found in the intact spinal cord. In both models, the transplanted NA-immunoreactive neurons formed extensive synaptic contacts with dendrites, spines and perikarya. The proportion of axodendritic and axospinous contacts was inverse in the two models. The first model thus reproduced more closely the normal synaptic pattern prefering dendritic targets, which could correspond to a better integration of the graft. In the second model, a partially NA-denervated spinal cord, there existed a competition between residual intrinsic and grafted neuron-derived fibres, which presumably affects synaptogenesis. In conclusion, the present study illustrate the complexity of cell interations conducting to the formation of a specific circuitry. Recognition phenomenon are likely modulated by space constraints, which ultimately shape-up the geometry of synaptic contacts. 相似文献
3.
4.
Britta Hedlund Maud Lorentz Peter Arhem 《Basic & clinical pharmacology & toxicology》1987,60(2):156-160
Abstract: Responses of NIE-115 neuroblastoma cells to application of carbachol were studied using intracellular recording techniques. Activation of muscarinic cholinergic receptors by carbachol resulted in a depolarization of the cells. The response was blocked by pirenzepine (1 μM) and by CoCl2 (5 mM), verapamil (10 μM) and gallopamil (10 μM), and prolonged by quinine (5 mM). It is suggested that muscarinic receptors increase the membrane calcium permeability, and that the influx of calcium activates calcium dependent potassium channels. 相似文献
5.
6.
7.
In order to define precisely the relation between descending monoaminergic systems and the motor system, we measured in the ventral horn of spinal cord of adult rats the variations of extracellular concentrations of 5-HT, 5-HIAA, DA and MHPG. Measurements were performed during rest, endurance running on a treadmill, and a post-exercise period, with microdialysis probes implanted permanently for 45 days. We found a slight decrease in both 5-HT and 5-HIAA during locomotion with a more marked decrease during the post-exercise period compared to the mean of rest values. In contrast, the concentration of DA and MHPG increased slightly during the exercise and decreased thereafter. These results, when compared with those of a previous study, which measured monoamines in the spinal cord white matter [C. Gerin, D. Bécquet, A. Privat, Direct evidence for the link between monoaminergic descending pathways and motor activity: I. A study with microdialysis probes implanted in the ventral funiculus of the spinal cord, Brain Res. 704 (1995) 191–201], highlight the complex regulation of the release of monoamines that occurs in the ventral horn. 相似文献
8.
9.
10.
N K?nig M J Drian A Privat N Lamandé N Parés-Herbuté M Schachner 《Neuroscience letters》1986,66(1):67-72
The possible influence of noradrenaline (NA) upon cell differentiation has been studied by comparing NA-supplemented cultures of foetal pallial cells with control cultures grown in normal medium. Two days after plating, the cultures were processed for immunocytochemical detection of either an adhesion molecule and marker of early stages of neuronal differentiation (L1) or a marker expressed at relatively late stages (gamma-enolase). In both cases, the NA supplement reduced the expression of the antigen. The effects were more clear-cut for the late than for the early marker. In conclusion, the NA supplement to the culture medium, in our model, seemed to have a 'differentiation regulating' rather than a 'neurotrophic' function sensu stricto. It remains to be clarified, however, to which extent this finding can be generalized to in vivo situations. 相似文献