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NAKADA KAZUTO YAO YAO MASHIMA JUN KATOH MASABUMI MIYAZAKI JUN-ICHI HIRABAYASHI TAMIO 《Journal of muscle research and cell motility》1998,19(2):169-177
To examine whether the expression pattern of fast-muscle type troponin-T (TnT) isoforms was fixed in cell lineage, breast
muscle pieces (pectoralis major) from chick embryos and young and adult chickens were grafted on to chorio-allantoic membrane
of 9-day-old chick embryos and cultured until the host embryos hatched out. Muscle fibre formation of the grafts was investigated
by histological and immunohistochemical methods with anti-fast-muscle type and anti-slow-muscle type TnT sera, and the expression
of fast-muscle type TnT in the grafts from chick embryos and young chickens was studied by SDS-polyacrylamide gel electrophoresis
(SDS-PAGE), two-dimensional SDS-PAGE, and immunoblotting. In the chorio-allantoic grafting, the breast muscle initially degenerated
forming pyknotic nuclei and hyaline cytoplasm. The surviving cells, which were supposed to be satellite cells, regenerated
new muscle fibres of the same type as those of the grafted muscle in respect of TnT isoform expression. Therefore, we considered
that the ability to express specific isoforms of TnT was fixed in the satellite cells, and that chorio-allantoic grafting
was a useful technique for studying muscle differentiation.
This revised version was published online in August 2006 with corrections to the Cover Date. 相似文献
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KEN UMETANI YOSHIWO OKAMOTO SABURO MASHIMA KOHEI ONO HIDEHIRO HOSAKA BIN HE 《Pacing and clinical electrophysiology : PACE》1998,21(11):2043-2054
Body surface Laplacian maps (BSLMs) have been previously reported to provide enhanced capability in localizing and resolving multiple spatially separate myocardial events. However, only a few studies have been reported on the clinical applications of BSLM. To test the clinical utility of BSLMs, BSLMs and body surface potential maps (BSPMs) during ventricular depolarization for complete right or left ventricular bundle branch block (CRBBB or CLBBB) were studied in ten patients in each group. As a control group, ten healthy subjects were also studied using the same procedure. One hundred and twenty-eight electrodes were placed uniformly over the entire chest and back of the subjects. BSLMs were computed from recorded potentials, using a numerical algorithm. The BSLMs showed multiple and more localized positive and negative activities compared with the BSPMs. In healthy subjects, the BSLMs showed multiple areas of positive activity overlying the RV, LV, and the RV outflow, and negative activity corresponding to RV free-wall breakthrough and LV anterolateral breakthrough sites, whereas the BSPMs could not separate RV and LV activities. In the patients with CRRRR, the BSLMs showed more localized areas of activity corresponding to the LV apex breakthrough and LV lateral breakthrough, and separated LV lateral and posterior activation. In the patients with CLBBB, the BSLMs showed multiple RV activation, and propagating activation of LV from lateral to posterior. The BSLMs appear to provide enhanced capability in detecting multiple ventricular electrical events associated with normal and abnormal conduction and a more detailed activation sequence of both ventricles in healthy subjects and in the patients with CRBBB and CLBBB. BSLM may provide an important alternative to other imaging modalities in localizing cardiac electrical activity noninvasively. 相似文献
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MASHIMA KAZUHIKO; EBIHARA SATOSHI; KASUYA HIDEKI 《Japanese journal of clinical oncology》1987,17(1):41-47
An acoustic screening method for indicating the possible presenceof laryngeal cancer was investigated. Three acoustic parameters,comprising perturbations in pitch-period and peak-amplitudesequences, and vocal noise, were measured from a sustained vowel,e, spoken by the subjects taking part in the investigation.Experiments to discriminate between normal and cancer groupswere performed with voice samples taken from 64 normal controlsubjects and 57 patients with laryngeal cancer. Experimentswere also carried out to test the perceptual significance ofthe three parameters. From the results, we have been able toconclude that their combined use will enable us to build anacoustic screening system for laryngeal cancer. 相似文献
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