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C. R. Irwin T. Myrillas M. Smyth J. Doogan C. Rice S. L. Schor 《Journal of oral pathology & medicine》1998,27(6):255-259
Fibroblasts incorporated within collagen gels induce a cell-mediated contraction of the gel to form a three-dimensional, tissue-like structure by a mechanism thought to mimic wound contraction in vivo . In this study a gel contraction model was used to investigate the ability of fibroblasts derived from adult gingiva, adult skin and fetal skin to organise a collagen matrix. In addition the effects of interleukin-1β (IL-1β) on the contraction process was also investigated. Over the concentration range 5-50 U/ml, IL-1β induced a statistically significant inhibition of gel contraction in all fibroblast cell types ( P <0.05), although fetal fibroblasts appeared least responsive and gingival fibroblasts most responsive to the inhibitory effects of this cytokine. Comparison of gel contraction by the different fibroblast strains indicated that fetal and gingival fibroblasts shared similar contraction kinetics. For the adult skin fibroblasts, three of five strains studied showed significantly diminished levels of gel contraction compared to fetal and gingival cells. This apparent difference in fibroblast phenotype may, at least in part, explain the fetal-like wound healing pattern seen in the oral mucosa. 相似文献
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Irwin L. Flink 《Brain structure & function》2002,205(3):235-244
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Four patients between 58 and 81 years of age undergoing investigation and endoscopic biopsy for gastric carcinoma also were subjected to direct-vision fine needle aspiration cytology of their mucosal lesions which yielded malignant cells. The relevance of this technique is discussed regarding both intrinsic and extrinsic lesions of the gastrointestinal tract. 相似文献
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F. P. Kolb K. B. Irwin J. R. Bloedel V. Bracha 《Experimental brain research. Experimentelle Hirnforschung. Expérimentation cérébrale》1997,114(2):255-270
Temporary inactivation of the cerebellar interposed nuclei was used to assess the role of the intermediate cerebellum in
the performance of forelimb cutaneo-muscular reflexes in the cat. The following types of reflexive responses were evaluated:
the classically conditioned and unconditioned forelimb withdrawal responses and the forelimb tactile placing, hopping and
magnet responses. The experiments tested the hypothesis that the intermediate cerebellum is involved in the performance of
all the above forelimb reflexes. The forelimb withdrawal reflex was classically conditioned in a newly developed paradigm
in which animals were first operantly conditioned to stand on four elevated platforms. Trained animals were microinjected
with a γ-aminobutyric acid (GABA) agonist, muscimol, in the interposed nuclei, and the effects of inactivation of the intermediate
cerebellar output on the forelimb reflexes were examined. The main findings of these experiments are that unilateral muscimol
inactivation of the interposed nuclei in the cat abolished the expression of the classically conditioned limb flexion reflex,
suppressed the performance of the unconditioned withdrawal reflex and, in parallel, downregulated the tactile placing, hopping
and magnet postural responses in the ipsilateral forelimb. These observations are inconsistent with concepts indicating exclusive
involvement of the intermediate cerebellum in the classically conditioned reflexes elicited by aversive stimuli. On the contrary,
they support the hypothesis of a more global involvement of this structure in learned and unlearned defensive flexion reflexes
and in automatic postural response systems.
Received: 29 July 1996 / Accepted: 26 September 1996 相似文献