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排序方式: 共有587条查询结果,搜索用时 31 毫秒
1.
Andersson M Rönnmark J Areström I Nygren PA Ahlborg N 《Journal of immunological methods》2003,283(1-2):225-234
Measurement of human serum molecules with two-site ELISA can be biased by the presence of human heterophilic anti-animal immunoglobulin antibodies (HAIA) that cause false-positive signals by cross-linking the monoclonal (mAb) and/or polyclonal antibodies (pAb) used for the pre- (capture) and post-analyte steps (detection). To evaluate a novel ELISA format designed to avoid interference by HAIA, a target-specific non-immunoglobulin (Ig) affinity protein (affibody) was used to replace one of the antibodies. First, a human IgA-binding affibody (Z(IgA)) selected by phage display technology from a combinatorial library of a single Staphylococcus aureus protein A domain was used. The detection range of IgA standard using an ELISA based on Z(IgA) for capture and goat pAb against IgA (pAb(IgA)) for detection was comparable with that of using pAb(IgA) for both capture and detection. Secondly, another affibody (Z(Apo)) was combined with mAb and used to detect recombinant human apolipoprotein A-1. The affibody/antibody ELISAs were also used to quantify human serum levels of IgA and apolipoprotein A1. To verify that human serum did not cause false-positive signals in the affibody/antibody ELISA format, the ability of human serum to cross-link affibodies, mAb (mouse or rat) and/or pAb (goat) displaying non-matched specificities was assessed; affibodies and antibodies were not cross-linked whereas all combinations of mAb and/or pAb were cross-linked. The combination of affibodies and antibodies for analysis of human serum molecules represents a novel two-site ELISA format which precludes false-positive signals caused by HAIA. 相似文献
2.
Quantitative detection of respiratory Chlamydia pneumoniae infection by real-time PCR 总被引:3,自引:0,他引:3 下载免费PDF全文
Kuoppa Y Boman J Scott L Kumlin U Eriksson I Allard A 《Journal of clinical microbiology》2002,40(6):2273-2274
Real-time PCR was evaluated as a quantitative diagnostic method for Chlamydia pneumoniae infection using different respiratory samples. Real-time PCR had efficiency equal to or better than that of nested touchdown PCR. This study confirmed sputum as the best sampling material to detect an ongoing C. pneumoniae infection. 相似文献
3.
Seyrantepe V Poupetova H Froissart R Zabot MT Maire I Pshezhetsky AV 《Human mutation》2003,22(5):343-352
Lysosomal sialidase (EC 3.2.1.18) has a dual physiological function; it participates in intralysosomal catabolism of sialylated glycoconjugates and is involved in cellular immune response. Mutations in the sialidase gene NEU1, located on chromosome 6p21.3, result in autosomal recessive disorder, sialidosis, which is characterized by the progressive lysosomal storage of sialylated glycopeptides and oligosaccharides. Sialidosis type I is a milder, late-onset, normosomatic form of the disorder. Type I patients develop visual defects, myoclonus syndrome, cherry-red macular spots, ataxia, hyperreflexia, and seizures. The severe early-onset form, sialidosis type II, is also associated with dysostosis multiplex, Hurler-like phenotype, mental retardation, and hepatosplenomegaly. We summarize information on the 34 unique mutations determined so far in the sialidase gene, including four novel missense and one novel nonsense mutations found in two Czech and two French sialidosis patients. The analysis of sialidase mutations in sialidosis revealed considerable molecular heterogeneity, reflecting the diversity of clinical phenotypes that make molecular diagnosis difficult. The majority of sialidosis patients have had missense mutations, many of which have been expressed; their effects on activity, stability, intracellular localization, and supramolecular organization of sialidase were studied. A structural model of sialidase allowed us to localize mutations in the sialidase molecule and to predict their impact on the tertiary structure and biochemical properties of the enzyme. 相似文献
4.
Cell surface-associated elongation factor Tu mediates the attachment of Lactobacillus johnsonii NCC533 (La1) to human intestinal cells and mucins 总被引:6,自引:0,他引:6 下载免费PDF全文
Granato D Bergonzelli GE Pridmore RD Marvin L Rouvet M Corthésy-Theulaz IE 《Infection and immunity》2004,72(4):2160-2169
The aim of this work was to identify Lactobacillus johnsonii NCC533 (La1) surface molecules mediating attachment to intestinal epithelial cells and mucins. Incubation of Caco-2 intestinal epithelial cells with an L. johnsonii La1 cell wall extract led to the recognition of elongation factor Tu (EF-Tu) as a novel La1 adhesin-like factor. The presence of EF-Tu at the surface of La1 was confirmed by analysis of purified outer surface protein extract by immunoblotting experiments, by electron microscopy, and by enzyme-linked immunosorbent assays of live bacteria. Furthermore, tandem mass spectrometry analysis proved that EF-TU was expressed at the La1 surface as an intact molecule. Using recombinant La1 EF-Tu protein, we were able to determine that its binding to intestinal cells and to mucins is pH dependent. Competition experiments suggested that EF-Tu has an important role in La1 mucin binding capacity. In addition, immunomodulation studies performed on HT29 cells showed that EF-Tu recombinant protein can induce a proinflammatory response in the presence of soluble CD14. Our in vitro results indicate that EF-Tu, through its binding to the intestinal mucosa, might participate in gut homeostasis. 相似文献
5.
A non-parametric bootstrap approach for analysing the statistical properties of SPECT and PET images
Buvat I 《Physics in medicine and biology》2002,47(10):1761-1775
Knowledge of the statistical properties of reconstructed single photon emission computed tomography (SPECT) and positron emission tomography (PET) images would be helpful for optimizing acquisition and image processing protocols. We describe a non-parametric bootstrap approach to accurately estimate the statistical properties of SPECT or PET images whatever the noise properties in the projections and the reconstruction algorithm. Using analytical simulations and real PET data, this method is shown to accurately predict the statistical properties, including the variance and covariance, of reconstructed pixel values for both linear (filtered backprojection) and non-linear (ordered subset expectation maximization) reconstruction algorithms. 相似文献
6.
Iréne?LundEmail author Thomas?Lundeberg Louise?Sandberg Cecilia?Norrbrink?Budh Jan?Kowalski Elisabeth?Svensson 《BMC medical research methodology》2005,5(1):31
Background:
Rating scales like the visual analogue scale, VAS, and the verbal rating scale, VRS, are often used for pain assessments both in clinical work and in research, despite the lack of a gold standard. Interchangeability of recorded pain intensity captured in the two scales has been discussed earlier, but not in conjunction with taking the influence of pain etiology into consideration. 相似文献7.
ABCA1 gene deletion protects against cerebral malaria: potential pathogenic role of microparticles in neuropathology 下载免费PDF全文
Combes V Coltel N Alibert M van Eck M Raymond C Juhan-Vague I Grau GE Chimini G 《The American journal of pathology》2005,166(1):295-302
The ATP-binding cassette transporter A1 (ABCA1) modulates the transbilayer distribution of phosphatidylserine at the outer leaflet of the plasma membrane. This external exposure of phosphatidylserine is a hallmark of microparticle production and is impaired in ABCA1(-/-) mice. In this study, we report about the complete resistance to cerebral malaria of these mice. On analysis of histological and systemic parameters we evidenced an impairment of cellular responses to Plasmodium berghei ANKA infection in ABCA1(-/-) mice, as shown by lower plasma tumor necrosis factor levels, a weaker up-regulation of endothelial adhesion molecules in brain microvessels, a reduced leukocyte sequestration, as well as an ablated platelet accumulation. Besides, the number and the procoagulant activity of microparticles were dramatically reduced in the plasma of ABCA1(-/-) compared to ABCA1(+/+) mice. Moreover, microparticles derived from Plasmodium berghei ANKA-infected ABCA1(+/+) mice induced a significant increase of tumor necrosis factor release by noninfected macrophages. In ABCA1(-/-) mice platelet and macrophage responses to vesiculation agonists were ablated and reduced, respectively. Altogether, by pointing out the ABCA1 transporter as a major element controlling cerebral malaria susceptibility, these data provide a novel insight into its pathophysiological mechanisms and are consistent with a pathogenic role of microparticles in this neurological syndrome. 相似文献
8.
Gerson M. Struik MSc MD PhD Bram Schermers MSc Ingeborg Mares MSc MD PhD Harold E. Lont MSc MD Jennifer W. Bradshaw MSc MD Bernard ten Haken MSc Ir PhD Theo J. M. Ruers MSc MD PhD Jurgen E. M. Mourik PhD Erwin Birnie MSc PhD Taco M. A. L. Klem MSc MD PhD 《The breast journal》2021,27(8):638-650
Wire-guided localization (WGL) is the standard of care in the surgical treatment of nonpalpable breast tumors. In this study, we compare the use of a new magnetic marker localization (MaMaLoc) technique to WGL in the treatment of early-stage breast cancer patients. Open-label, single-center, randomized controlled trial comparing MaMaLoc (intervention) to WGL (control) in women with early-stage breast cancer. Primary outcome was surgical usability measured using the System Usability Scale (SUS, 0–100 score). Secondary outcomes were patient reported, clinical, and pathological outcomes such as retrieval rate, operative time, resected specimen weight, margin status, and reoperation rate. Thirty-two patients were analyzed in the MaMaLoc group and 35 in the WGL group. Patient and tumor characteristics were comparable between groups. No in situ complications occurred. Retrieval rate was 100% in both groups. Surgical usability was higher for MaMaLoc: 70.2 ± 8.9 vs. 58.1 ± 9.1, p < 0.001. Patients reported higher overall satisfaction with MaMaLoc (median score 5/5) versus WGL (score 4/5), p < 0.001. The use of magnetic marker localization (MaMaLoc) for early-stage breast cancer is effective and has higher surgical usability than standard WGL. 相似文献
9.
Lucie Matrat MD Mathias Ruiz MD Emmanuelle Ecochard-Dugelay MD Irène Loras-Duclaux MD Stéphanie Marotte MD Sophie Heissat MD Pierre Poinsot MD Anne-Laure Sellier-Leclerc MD Justine Bacchetta MD PhD Laurence Dubourg MD PhD Noël Peretti MD PhD 《JPEN. Journal of parenteral and enteral nutrition》2022,46(1):180-189
10.
J. Th. M. Groenewegen Ir. P. J. J. De Meijer 《Pharmacy World & Science》1980,2(1):965-972
Samenvatting Een systeem voor dunnelaagchromatografie wordt beschreven met behulp waarvan twaalf verschillende benzodiazepinen kunnen worden geïdentificeerd en waarmee tevens eventuele ontledingsprodukten kunnen worden aangetoond. Er wordt gebruik gemaakt vanhptlc Silica Gel 60 F254 plaatjes en als loopvloeistof wordt tolueen-methanol (96 + 6) gebruikt.De vlekjes kunnen direct onderuv-licht worden waargenomen; ze kunnen nader worden geïdentificeerd na bespuiten met zwavelzuur en/of het reagens van Bratton Marshall. Van enkele benzodiazepinen kan de identiteit echter pas worden vastgesteld nadat ze zijn gehydrolyseerd. 相似文献