Improvement of bone repair with l-PRF and bovine bone in calvaria of rats. histometric and immunohistochemical study

Clinical Oral Investigations - The effect of leucocyte- and platelet-rich fibrin (L-PRF), associated with DBBM (deproteinized bovine bone mineral; Bio-Oss®) was investigated and compared with...     

Selective Cytotoxicity to Human Leukemic Myeloblasts Produced by Oligodeoxyribonucleotide Phosphorothioates Complementary to p53 Nucleotide Sequences

Cells were treated in vitro with oligodeoxyribonucleotide phosphorothioates (ODNs) complementary to sites common to both wild-type and mutant p53 nucleotide sequences. Acute myelogenous leukemia (AML) blasts from peripheral blood were exposed to four different p53 ODNs and showed anti-leukemic effects in suspension culture. This effect continued after removal of the ODN from the medium. Blocking of self-renewal of the leukemic blast stem cells in secondary plating of cells from cloning assays by two of the p53 ODNs was also observed. Control ODNs had no effect on leukemic blasts. Treatment of normal bone marrow cells with the four p53 ODNs did not influence their growth, nor was there any effect by the p53 ODNs on the leukemic cell-line, HL60, that does not express p53. These data suggest that p53 ODNs are selectively toxic to primary myelogenous blasts and may be therapeutically useful in AML.     

The status of hematopoietic stem cell transplantation in lysosomal storage disease

Lysosomal storage diseases are a group of disorders which have in common an inherited defect in lysosomal function—in most cases, a missing intralysosomal enzyme. Research into potential treatment options for this group of disorders has focused on enzyme replacement. Over the past two decades, hematopoietic stem cell transplantation has been used with increasing frequency to treat patients with lysosomal storage disease by providing a population of cells with the capacity to produce the missing enzyme. The success of marrow transplantation depends on the specific enzyme deficiency and the stage of the disease. Generally, visceral symptoms can be improved, whereas skeletal lesions remain relatively unaffected. The effect on neurologic symptoms varies. Hematopoietic stem cell transplantation remains a viable treatment option in those lysosomal storage diseases where data supportive of disease stabilization or amelioration are known. Early transplantation is the goal so that enzyme replacement may occur before extensive central nervous system injury becomes evident. When inadequate clinical data are available, the decision to perform transplantation requires experimental data demonstrating that the enzyme in question is both excreted from normal cells and taken up by affected cells as evidenced by elimination of storage material in vitro.     

Hurler syndrome with special reference to histologic abnormalities of the growth plate

Hurler syndrome is a mucopolysaccharide disorder resulting from an heritable deficiency in alpha-L-iduronidase, an enzyme required in the catabolism of heparan sulfate and dermatan sulfate glycosaminoglycan (GAGs). The resultant intracellular accumulation of GAG leads to disruption of the intracellular and extracellular environment and dysfunction of multiple organ systems. Among the most noted manifestations of this disease is disproportionate short trunk dwarfism, which develops during the first years of life. Histochemical and electron-microscopic observations on a 30-month-old child with Hurler syndrome showed marked irregularities in chondrocyte orientation within the growth plate, along with disruption of the normal columnar architecture. Vacuolization with enlargement of the cellular border was the characteristic ultrastructural finding. An heritable abnormality in the enzymatic degradation of structural glycosaminoglycans leads to profound disruption of the normal mechanisms of growth and development.     

Primary and radiation induced skull base osteosarcoma: a systematic review of clinical features and treatment outcomes

Journal of Neuro-Oncology - We aim to systematically review and summarize the demographics, clinical features, management strategies, and clinical outcomes of primary and radiation-induced...     

Three cases of severe verapamil poisoning

Three cases of verapamil intoxication are described. The first two patients took 6.4 g verapamil each and the third one took 0.8 g of verapamil and alcohol. All three developed a third-degree atrioventricular dissociation, the first patient developed a slow idioventricular rhythm without atrial activity, the second a temporary asystole. All were hypotensive, had anuria and shock, the first two were unconscious for several hours. The first one was treated with artificial cardiac pacing, orciprenaline and calcium gluconate, the second with cardiac pacing and orciprenaline. The third patient received fluids and a dopamine infusion. In the first case sinus rhythm returned 31 h after the swallowing of tablets, in the second after 32 h and in the third after 10 h. Pacing with intensive monitoring and care seemed to be the most beneficial treatments in these cases.     

Comparison of the anti-neoplastic effects of dirhodium(II) tetrapropionate and its adducts with nicotinate and isonicotinate anions in mice bearing Ehrlich tumors

Rhodium(II) propionate, [Rh2(prop)4], and its adduct with nicotinate (nic-) and isonicotinate (isonic-) anions, [Rh2(prop)4(nic)2](2-) and [Rh2(prop)4(isonic)2](2-), respectively, were prepared for study. The compound effects on the survival rate of mice bearing Ehrlich ascites tumors were tested and presented in the form of a survival table, and analyzed by the Mantel-Haenszel chi-square test for N animals in each group. The survival rates of animals were significantly higher than that of control group (P<0.001) without distinguishing among the experimental groups. The estimated probability for an animal in the control group to survive up to the end of the observation period (30 days) was below 33%, whereas the animal groups in the treated group with complex, and its nicotinate and isonicotinate groups showed 85%, 85% and 90%, respectively, of surviving over the same period. The T/C values (survival average of the animals treated group/survival average of the animals control group) were obtained for each compounds being for the dirhodium propionate T/C=250, and for its adducts with nicotinate and isonicotinate anions, 267 and 264, respectively.