全文获取类型
收费全文 | 1001篇 |
免费 | 65篇 |
国内免费 | 2篇 |
专业分类
耳鼻咽喉 | 5篇 |
儿科学 | 47篇 |
妇产科学 | 21篇 |
基础医学 | 101篇 |
口腔科学 | 9篇 |
临床医学 | 117篇 |
内科学 | 276篇 |
皮肤病学 | 20篇 |
神经病学 | 105篇 |
特种医学 | 47篇 |
外科学 | 102篇 |
综合类 | 15篇 |
一般理论 | 2篇 |
预防医学 | 56篇 |
眼科学 | 14篇 |
药学 | 64篇 |
1篇 | |
肿瘤学 | 66篇 |
出版年
2023年 | 4篇 |
2022年 | 7篇 |
2021年 | 22篇 |
2020年 | 18篇 |
2019年 | 15篇 |
2018年 | 27篇 |
2017年 | 20篇 |
2016年 | 20篇 |
2015年 | 23篇 |
2014年 | 30篇 |
2013年 | 31篇 |
2012年 | 54篇 |
2011年 | 47篇 |
2010年 | 33篇 |
2009年 | 31篇 |
2008年 | 46篇 |
2007年 | 40篇 |
2006年 | 43篇 |
2005年 | 52篇 |
2004年 | 56篇 |
2003年 | 43篇 |
2002年 | 43篇 |
2001年 | 30篇 |
2000年 | 48篇 |
1999年 | 41篇 |
1998年 | 20篇 |
1997年 | 27篇 |
1996年 | 14篇 |
1995年 | 10篇 |
1994年 | 15篇 |
1993年 | 11篇 |
1992年 | 13篇 |
1991年 | 4篇 |
1990年 | 8篇 |
1989年 | 14篇 |
1988年 | 11篇 |
1987年 | 13篇 |
1986年 | 12篇 |
1985年 | 8篇 |
1984年 | 5篇 |
1983年 | 5篇 |
1982年 | 4篇 |
1981年 | 7篇 |
1977年 | 9篇 |
1976年 | 6篇 |
1974年 | 4篇 |
1969年 | 2篇 |
1967年 | 2篇 |
1966年 | 3篇 |
1964年 | 2篇 |
排序方式: 共有1068条查询结果,搜索用时 15 毫秒
1.
2.
Andrea Vliz Ramírez Susanne Krmer Strenger Melissa Solar Lpez Pamela Muoz Cortes Camila Corral Núez 《Special care in dentistry》2019,39(2):225-230
Cerebral palsy (CP) is a condition caused by brain damage before, during, or shortly after birth. Communication can be a challenge when treating patients with CP. Some patients can communicate verbally, while others use augmentative alternative communication tools or have individualized means of communication. Therefore, professional dental treatment in individuals with CP is challenging, especially if the patient is affected by dental trauma and requires emergency treatment. This report shows how individualized communication skills assessment allowed us to successfully manage a 9‐year‐old patient with CP, who suffered extrusive luxation of the permanent lower incisor. In the present case, the teeth were repositioned briefly after the trauma had occurred and then stabilized with a flexible splint according to international guidelines. The teeth remained vital and periodontal repair was observed during the 4‐year follow‐up. 相似文献
3.
Immunophenotypic, genomic and clinical characteristics of blast crisis of chronic myelogenous leukaemia 总被引:1,自引:0,他引:1
J. M. Hernández R. González-Sarmiento C. Martin M. González I. Sánchez J. Corral A. Orfao M. C. Canizo J. F. san Miguel A. López-borrasca 《British journal of haematology》1991,79(3):408-414
We have studied phenotypic and clinical features in a consecutive series of 45 patients with chronic myelogenous leukaemia (CML) in blast crisis (BC). In addition, in 22 of these patients we have analysed the genotypic characteristics including immunoglobulin, T-cell receptor (TCR) and major breakpoint cluster region (M-bcr) gene organization. The granulomonocytic and megakaryoblastic lineages are the most commonly involved in these BC of CML (33% and 33% of cases, respectively); only 18% of our cases displayed a lymphoid phenotype. Moreover, both morphological and immunophenotypic studies revealed the frequent coexistence of two or three cell populations, especially when the megakaryoblast component is involved. The lymphoid BC displayed the highest incidence of complete remissions although this was not associated with a longer survival. Only minor differences between the different myeloid subgroups were observed. Immunoglobulin heavy chain (IgH) gene rearrangement was found in five of the six lymphoid BC and in one myeloid BC. Only one case showed k light chain gene rearrangement. In all but one myeloid BC the TCR-beta gene was in germline configuration. The TCR-gamma gene was rearranged in all lymphoid and one myeloid BC, while TCR-delta gene rearrangement was detected in 67% and 16% of the lymphoid and myeloid BC, respectively. Most of the lymphoid BC (4/5) had the M-bcr breakpoint in subregion 3, while the myeloid BC had the breakpoint either in subregion 2 or 3. No differences between the different myeloid phenotypic subgroups were observed in relation to breakpoint. 相似文献
4.
M.F. López-Fernández MD C. López-Berges M. Corral J.R. García-Talavera A. López Borrasca and J. Batlle 《Vox sanguinis》1987,52(1-2):15-19
The multimeric structure of von Willebrand factor (vWF) and its ristocetin-induced binding to platelets, using a simple and very sensitive radiomonoclonal antibody-labeled vWF method, was compared in normal plasma, single-donor cryoprecipitate (CP) and five different antihemophilic factor (AHF) concentrates. All the AHF showed a lack of larger vWF multimers, an abnormal 'triplet' pattern, and much lower vWF binding to platelets than that of plasma or CP, vWF being the lowest for those with a lesser proportion of larger vWF multimers. These results suggest that the combination of vWF multimeric analysis and the radiomonoclonal-labeled vWF method may be very useful in the assessment of AHF preparations. 相似文献
5.
6.
Rada P Tucci S Teneud L Paez X Perez J Alba G García Y Sacchettoni S del Corral J Hernandez L 《Journal of chromatography. B, Biomedical sciences and applications》1999,735(1):1-10
Due to its low electrophoretic mobility, few studies have been able to measure gamma aminobutyric acid (GABA) in biological samples by means of capillary zone electrophoresis. Nevertheless, in micellar electrokinetic chromatography (MEKC) by adding a surfactant to the mobile phase separation can be carried out on the basis of the partition coefficient of the molecules rather than their electrophoretic mobility. In the present study microdialysis coupled to MEKC with laser induced fluorescence detection was used to successfully monitor GABA from cerebrospinal fluid and plasma dialysates. Moreover, we monitored changes in extracellular GABA from a human brain. Microdialysis samples were collected from a Parkinson's disease patient undergoing a thallamotomy as part of her treatment. Significant decreases in extracellular GABA were detected during high frequency electrical stimulation and following a thermolesion of the thalamus. These results demonstrate the feasibility of MEKC coupled to laser-induced fluorescence detection in resolving neutral amino acids, specifically GABA, from different human body fluids. 相似文献
7.
8.
Changes in the human immunodeficiency virus p7-p1-p6 gag gene in drug-naive and pretreated patients
下载免费PDF全文
![点击此处可从《Journal of clinical microbiology》网站下载免费的PDF全文](/ch/ext_images/free.gif)
Resistance to antiretroviral agents often results from mutations within the human immunodeficiency virus (HIV) pol gene. Moreover, insertions within the p6 gag-pol region have recently been found to be involved with resistance to nucleoside analogs. Overall, we found that 21% of 156 specimens collected from HIV-infected individuals (17.6% from 74 drug-naive patients and 24.4% from 82 pretreated patients) harbored these insertions. Insertions around the KQE (Lys-Gln-Glu) motif were found in 12.2% of the pretreated patients but in none of the drug-naive subjects (P = 0.002). In contrast, insertions around the PTAP (Prol-Thre-Ala-Prol) motif were seen at similar rates ( approximately 15%) among drug-naive and pretreated patients, which supports the idea that they may be natural polymorphisms. 相似文献
9.
Different modulation by histamine of IL-4 and interferon-gamma (IFN-γ) release according to the phenotype of human Th0, Th1 and Th2 clones
下载免费PDF全文
![点击此处可从《Clinical and experimental immunology》网站下载免费的PDF全文](/ch/ext_images/free.gif)
Histamine, an important inflammatory mediator in allergic diseases and asthma, has been reported to have modulator effects on T cells, suggesting that the bronchial microenvironment may regulate the function of resident T cells. We examined the effect of histamine on the release of the Th2-associated cytokines IL-4 and IL-5 and the Th1-associated cytokine IFN-γ by 30 CD4+ T cell clones from peripheral blood or bronchial biopsy of one atopic subject. Based on the IL-4/IFN-γ ratio, the clones were ascribed to the Th2 (ratio >1), Th0 (ratio 0.1 and 1) or Th1 (ratio <0.1) phenotype. Histamine inhibited IFN-γ production by Th1-like cells (P<0.02, Kruskall–Wallis), especially from bronchial biopsy, but had no effect on IL-4 release. Regarding Th0 clones, histamine inhibited IL-4 production (P<0.02) in a dose-dependent manner and slightly inhibited IFN-γ production, but had no effect on Th2-like cells. Histamine had a heterogeneous and insignificant effect on IL-5 production. The H2-receptor antagonist ranitidine completely reversed the inhibition of IL-4 and IFN-γ production, whereas the agonist dimaprit mimicked this effect. In contrast, H1- and H3-receptor agonists and antagonists had no significant effect. These data demonstrate that histamine has different effects on IL-4 and IFN-γ release by T helper cells according to their phenotype via H2-receptors. This study extends the immunomodulatory effects of histamine which may contribute to the perpetuation of airway inflammation in asthma. 相似文献
10.