排序方式: 共有21条查询结果,搜索用时 15 毫秒
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Cheng-Chi Wang Juine-Ruey Chen Yung-Chieh Tseng Che-Hsiung Hsu Yu-Fu Hung Shih-Wei Chen Chin-Mei Chen Kay-Hooi Khoo Ting-Jen Cheng Yih-Shyun E. Cheng Jia-Tsrong Jan Chung-Yi Wu Che Ma Chi-Huey Wong 《Proceedings of the National Academy of Sciences of the United States of America》2009,106(43):18137-18142
Recent cases of avian influenza H5N1 and the swine-origin 2009 H1N1 have caused a great concern that a global disaster like the 1918 influenza pandemic may occur again. Viral transmission begins with a critical interaction between hemagglutinin (HA) glycoprotein, which is on the viral coat of influenza, and sialic acid (SA) containing glycans, which are on the host cell surface. To elucidate the role of HA glycosylation in this important interaction, various defined HA glycoforms were prepared, and their binding affinity and specificity were studied by using a synthetic SA microarray. Truncation of the N-glycan structures on HA increased SA binding affinities while decreasing specificity toward disparate SA ligands. The contribution of each monosaccharide and sulfate group within SA ligand structures to HA binding energy was quantitatively dissected. It was found that the sulfate group adds nearly 100-fold (2.04 kcal/mol) in binding energy to fully glycosylated HA, and so does the biantennary glycan to the monoglycosylated HA glycoform. Antibodies raised against HA protein bearing only a single N-linked GlcNAc at each glycosylation site showed better binding affinity and neutralization activity against influenza subtypes than the fully glycosylated HAs elicited. Thus, removal of structurally nonessential glycans on viral surface glycoproteins may be a very effective and general approach for vaccine design against influenza and other human viruses. 相似文献
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Modulation of calmodulin by UV and X-rays in primary human endothelial cell cultures 总被引:1,自引:0,他引:1
Watson CA Chang-Liu CM Woloschak GE 《International journal of radiation biology》2000,76(11):1455-1461
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Regulation of protein kinase C by ionizing radiation 总被引:3,自引:0,他引:3
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