全文获取类型
收费全文 | 319篇 |
免费 | 21篇 |
国内免费 | 1篇 |
专业分类
儿科学 | 7篇 |
妇产科学 | 13篇 |
基础医学 | 43篇 |
口腔科学 | 3篇 |
临床医学 | 44篇 |
内科学 | 39篇 |
皮肤病学 | 6篇 |
神经病学 | 35篇 |
特种医学 | 22篇 |
外科学 | 33篇 |
综合类 | 1篇 |
预防医学 | 33篇 |
眼科学 | 2篇 |
药学 | 35篇 |
中国医学 | 3篇 |
肿瘤学 | 22篇 |
出版年
2023年 | 2篇 |
2022年 | 6篇 |
2021年 | 15篇 |
2020年 | 13篇 |
2019年 | 10篇 |
2018年 | 18篇 |
2017年 | 10篇 |
2016年 | 10篇 |
2015年 | 13篇 |
2014年 | 17篇 |
2013年 | 25篇 |
2012年 | 31篇 |
2011年 | 39篇 |
2010年 | 16篇 |
2009年 | 8篇 |
2008年 | 22篇 |
2007年 | 15篇 |
2006年 | 17篇 |
2005年 | 13篇 |
2004年 | 10篇 |
2003年 | 15篇 |
2002年 | 7篇 |
2001年 | 1篇 |
2000年 | 1篇 |
1999年 | 1篇 |
1998年 | 1篇 |
1997年 | 1篇 |
1993年 | 2篇 |
1968年 | 2篇 |
排序方式: 共有341条查询结果,搜索用时 15 毫秒
1.
Bojana Stefanovic Wolfram Schwindt Mathias Hoehn Afonso C Silva 《Journal of cerebral blood flow and metabolism》2007,27(4):741-754
The cerebrovascular coupling under neuronal nitric oxide synthase (nNOS) inhibition was investigated in alpha-chloralose anesthetized rats. Cerebral blood flow (CBF), cerebral blood volume (CBV), and blood oxygenation level dependent (BOLD) responses to electrical stimulation of the forepaw were measured before and after an intraperitoneal bolus of 7-nitroindazole (7-NI), an in vivo inhibitor of the neuronal isoform of nitric oxide synthase. Neuronal activity was measured by recording somatosensory-evoked potentials (SEPs) via intracranial electrodes. 7-Nitroindazole produced a significant attenuation of the activation-elicited CBF (P<10(-6)), CBV (P<10(-6)), and BOLD responses (P<10(-6)), without affecting the baseline perfusion level. The average DeltaCBF was nulled, while DeltaBOLD and DeltaCBV decreased to approximately 30% of their respective amplitudes before 7-NI administration. The average SEP amplitude decreased (P<10(-5)) to approximately 60% of its pretreatment value. These data describe a pharmacologically induced uncoupling between neuronal and hemodynamic responses to functional activation, and provide further support for the critical role of neuronally produced NO in the cerebrovascular coupling. 相似文献
2.
3.
Dragana Arsenijevic Bojana Stojanovic Jelena Milovanovic Aleksandar Arsenijevic Milos Simic Marija Pergal Igor Kodranov Olga Cvetkovic Danilo Vojvodic Elizabeta Ristanovic Dragan Manojlovic Marija Milovanovic Nebojsa Arsenijevic 《Nutrients》2021,13(3)
The main biologically active components of plants belonging to the genus Allium, responsible for their biological activities, including anti-inflammatory, antioxidant and immunomodulatory, are organosulfur compounds. The aim of this study was to synthetize the mixture of dipropyl polysulfides (DPPS) and to test their biological activity in acute hepatitis. C57BL/6 mice were administered orally with DPPS 6 h before intravenous injection of Concanavalin A (ConA). Liver inflammation, necrosis and hepatocytes apoptosis were determined by histological analyses. Cytokines in liver tissue were determined by ELISA, expression of adhesive molecules and enzymes by RT PCR, while liver mononuclear cells were analyzed by flow cytometry. DPPS pretreatment significantly attenuated liver inflammation and injury, as evidenced by biochemical and histopathological observations. In DPPS-pretreated mice, messenger RNA levels of adhesion molecules and NADPH oxidase complex were significantly reduced, while the expression of SOD enzymes was enhanced. DPPS pretreatment decreased protein level of inflammatory cytokines and increased percentage of T regulatory cells in the livers of ConA mice. DPPS showed hepatoprotective effects in ConA-induced hepatitis, characterized by attenuation of inflammation and affection of Th17/Treg balance in favor of T regulatory cells and implicating potential therapeutic usage of DPPS mixture in inflammatory liver diseases. 相似文献
4.
5.
6.
Katarina Gojko Kanurić Spasenija Danilo Milanović Bojana Branko Ikonić Eva Stjepan Lončar Mirela Dragoljub Iličić Vladimir Radovan Vukić Dajana Vukota Vukić 《Yao wu shi pin fen xi = Journal of food and drug analysis.》2018,26(4):1229-1234
The aim of this research was to investigate the effect of new, non-conventional starter culture on the kinetics of the lactose transformation during milk fermentation by kombucha, at pH 5.8; 5.4; 5.1; 4.8; and 4.6, at two different temperatures 37 °C and 42 °C. Milk fermentation at 42 °C lasted significantly shorter (about 5 h, 30 min) compared to the fermentation at 37 °C. Changes of lactose concentration at the both temperatures are consisting of two retaining stages and very steep decline in–between. The analysis of the rate curves showed that the reaction rate passes through the maximum after 9 h, 30 min at 37 °C and after 4 h at 42 °C. The sigmoidal saturation curve indicates a complex kinetics of lactose fermentation by kombucha starter. 相似文献
7.
Tatjana Adzic-Vukicevic Aleksandra Barac Ana Blanka-Protic Marija Laban-Lazovic Bojana Lukovic Vesna Skodric-Trifunovic Salvatore Rubino 《Infection》2018,46(3):357-363
Introduction
Non-tuberculous mycobacteria (NTM) are ubiquitous organisms associated with various infections. The aim of the study was to determine the most relevant clinical characteristics of NTM during the 7-year period.Methodology
A retrospective study of NTM infections was conducted between January 2009 and December 2016. The American Thoracic Society/Infectious Disease Society of America criteria were used to define cases of pulmonary or an extrapulmonary site.Results
A total of 85 patients were included in the study. Pulmonary cases predominated 83/85 (98%), while extrapulmonary NTM were present in 2/95 (2%) patients. Overall, ten different NTM species were isolated. The most common organisms were slow-growing mycobacteria (SGM) presented in 70/85 (82.35%) patients. Isolated SGM strains were Mycobacterium avium complex (MAC) in 25/85 (29.41%) patients, M. xenopi in 20/85 (23.53%) patients, M. kansasii in 15/85 (17.65%) patients and M. peregrinum and M. gordonae in 5/85 (5.88%) patients each. Isolated rapid-growing mycobacteria (RGM) strains were M. abscessus in 8/85 (9.41%) patients, M. fortuitum in 4/85 (4.71%) patients and M. chelonae in 3/85 (3.53%) patients. Almost all patients (98%; 83/85) had comorbidities. Among 75 (88.24%) patients who completed follow-up, 59 (69.41%), 10 (11.76%) and 6 (7%), were cured, experienced relapse and died, respectively.Conclusion
In the present study, pulmonary NTM infections were more frequent compared to extrapulmonary disease forms. SGM were most common isolates with MAC pulmonary disease the most frequently found. Comorbidities have an important role in NTM occurrence. Further investigation should focus on an NTM drug susceptibility testing.8.
Leticia Díez-Quijada Klara Hercog Martina tampar Metka Filipi
Ana M. Camen ngeles Jos Bojana
egura 《Toxins》2020,12(12)
Simultaneous occurrence of cylindrospermopsin (CYN) and microcystin-LR (MCLR) has been reported in the aquatic environment and thus human exposure to such mixtures is possible. As data on the combined effects of CYN/MCLR are scarce, we aimed to investigate the adverse effects related to genotoxic activities induced by CYN (0.125, 0.25 and 0.5 µg/mL) and MCLR (1 µg/mL) as single compounds and their combinations in HepG2 cells after 24 and 72 h exposure. CYN and CYN/MCLR induced DNA double-strand breaks after 72 h exposure, while cell cycle analysis revealed that CYN and CYN/MCLR arrested HepG2 cells in G0/G1 phase. Moreover, CYN and the combination with MCLR upregulated CYP1A1 and target genes involved in DNA-damage response (CDKN1A, GADD45A). Altogether, the results showed that after 72 h exposure genotoxic activity of CYN/MCLR mixture was comparable to the one of pure CYN. On the contrary, MCLR (1 µg/mL) had no effect on the viability of cells and had no influence on cell division. It did not induce DNA damage and did not deregulate studied genes after prolonged exposure. The outcomes of the study confirm the importance of investigating the combined effects of several toxins as the effects can differ from those induced by single compounds. 相似文献
9.
10.