全文获取类型
收费全文 | 11497篇 |
免费 | 553篇 |
国内免费 | 71篇 |
专业分类
耳鼻咽喉 | 89篇 |
儿科学 | 349篇 |
妇产科学 | 301篇 |
基础医学 | 1483篇 |
口腔科学 | 232篇 |
临床医学 | 686篇 |
内科学 | 2738篇 |
皮肤病学 | 254篇 |
神经病学 | 1058篇 |
特种医学 | 240篇 |
外科学 | 1646篇 |
综合类 | 33篇 |
一般理论 | 1篇 |
预防医学 | 889篇 |
眼科学 | 412篇 |
药学 | 577篇 |
中国医学 | 27篇 |
肿瘤学 | 1106篇 |
出版年
2023年 | 48篇 |
2022年 | 125篇 |
2021年 | 337篇 |
2020年 | 155篇 |
2019年 | 258篇 |
2018年 | 301篇 |
2017年 | 192篇 |
2016年 | 208篇 |
2015年 | 266篇 |
2014年 | 372篇 |
2013年 | 499篇 |
2012年 | 761篇 |
2011年 | 858篇 |
2010年 | 446篇 |
2009年 | 441篇 |
2008年 | 764篇 |
2007年 | 742篇 |
2006年 | 725篇 |
2005年 | 714篇 |
2004年 | 666篇 |
2003年 | 593篇 |
2002年 | 586篇 |
2001年 | 226篇 |
2000年 | 252篇 |
1999年 | 179篇 |
1998年 | 96篇 |
1997年 | 80篇 |
1996年 | 81篇 |
1995年 | 80篇 |
1994年 | 47篇 |
1993年 | 47篇 |
1992年 | 113篇 |
1991年 | 93篇 |
1990年 | 69篇 |
1989年 | 81篇 |
1988年 | 71篇 |
1987年 | 57篇 |
1986年 | 48篇 |
1985年 | 49篇 |
1984年 | 46篇 |
1983年 | 36篇 |
1982年 | 21篇 |
1981年 | 26篇 |
1980年 | 21篇 |
1979年 | 31篇 |
1978年 | 17篇 |
1977年 | 18篇 |
1975年 | 20篇 |
1974年 | 22篇 |
1973年 | 21篇 |
排序方式: 共有10000条查询结果,搜索用时 125 毫秒
1.
2.
3.
4.
5.
M. A. García-Pérez C. Climent P. Briones M. A. Vilaseca M. Rodés V. Rubio 《Journal of inherited metabolic disease》1997,20(6):769-777
Mutations P225L and P225R were identified in codon 225 of the gene for ornithine transcarbamylase (OTC) in two patients with the neonatal form of OTC deficiency. The mutations occur at a CpG dinucleotide and eliminate a unique MspI restriction site in exon 7 of the OTC gene. They do not alter existing splice sites or create new sites, as judged from the nucleotide sequence. Both mutations are associated with undetectable levels of OTC antigen in liver homogenates, and with either complete lack of OTC activity (P225R mutation) or very small residual activity (0.15% of normal in the P225L mutation). The residual activity observed with P225L exhibits normal pH dependence, little or no increases in the Km values for ornithine and carbamoyl phosphate and normal stability at either 37°C or, in the presence of 0.66 mol/L urea, at 0°C. The latter conditions were used to examine whether the P225L mutation favours dissociation of the active OTC trimer. Given the normal stability and lack of tendency to dissociation of the mutant enzyme, it appears likely that the dramatic reduction in the level of OTC protein is due to inefficient conversion of the mutant OTC precursor polypeptide (pOTC) into the correctly localized, appropriately folded, mature enzyme trimer, suggesting degradation of pOTC in transit to the mitochondria. 相似文献
6.
Fernanda Martini Alfredo Corallini Veronica Balatti Silvia Sabbioni Cecilia Pancaldi Mauro Tognon 《Infectious agents and cancer》2007,2(1):13-12
Simian virus 40 (SV40) is a monkey virus that was administered to human populations by contaminated vaccines which were produced
in SV40 naturally infected monkey cells. 相似文献
7.
Carmen Guillén Ponce María Pilar Garrido López María José Molina Garrido Gema Muñoz Molina Alfredo Carrato 《Clinical & translational oncology》2007,9(1):53-55
Pituitary metastases of solid tumours are infrequent, specially as a first manifestation. When they happen, they are usually
due to breast or lung cancer and are asymptomatic or produce diabetes insipidus. It is very strange that they produce hormonal
deficiency.
We present a case report of a bronchogenic adenocarcinoma in a 65-year-old man which began with panhypopituitarism, diabetes
insipidus and visual alterations. Magnetic resonance imaging revealed a large sellar mass, with clivus infiltration and invading
the right cavernous sinus. The biopsy result was adenocarcinoma metastases from lung cancer. 相似文献
8.
Enza-Maria Valente Anjum Misbahuddin Francesco Brancati Mark R Placzek Barbara Garavaglia Sergio Salvi Andrea Nemeth Charles Shaw-Smith Nardo Nardocci Anna-Rita Bentivoglio Alfredo Berardelli Roberto Eleopra Bruno Dallapiccola Thomas T Warner 《Movement disorders》2003,18(9):1047-1051
The epsilon-sarcoglycan gene (SGCE) on human chromosome 7q21 has been reported to be a major locus for inherited myoclonus-dystonia. Linkage to the SGCE locus has been detected in the majority of families tested, and mutations in the coding region have been found recently in families with autosomal dominant myoclonus-dystonia. To evaluate the relevance of SGCE in myoclonus-dystonia, we sequenced the entire coding region of the epsilon-sarcoglycan gene in 16 patients with either sporadic or familial myoclonus-dystonia. No mutations were found. This study suggests that epsilon-sarcoglycan does not play an important role in sporadic myoclonus-dystonia and supports genetic heterogeneity in familial cases. 相似文献
9.
J V Pernía Morán P Rubio Pascual C Siminiani I Lahoz 《Revista espa?ola de anestesiología y reanimación》1990,37(2):98-100
We report the case of a 32-year-old multipara who presented preeclampsia on the fourth day after childbirth without receiving proper treatment that progressed to eclampsia 4 days later. Pregnancy and delivery had been uneventful. The patient presented proteinuria (30 mg/dl), serum total proteins 5.3 g/dl and serum albumin 3.3 g/dl. Blood pressure was controlled with methyldopa, 500 mg at six-hour intervals by intravenous route. The patient presented hypoxemia secondary to bilateral pleural effusion and aspirative pneumonia requiring mechanical ventilation and invasive hemodynamic monitoring. Treatment with cefotaxime, 1 g at six-hour intervals by intravenous route and clindamycin, 600 mg at six-hour intervals by intravenous route was initiated. Sedation was maintained with thiopental sodium, 3 mg/kg/hour in continuous infusion. At dismission, the patient was completely recovered from her clinical picture and needed no antihypertensive therapy. Physiopathologic features and the aforementioned complications are discussed with particular reference to differential diagnosis. 相似文献
10.
Alfredo Marzano Silvia Gaia Valeria Ghisetti Silvia Carenzi Alberto Premoli Wilma Debernardi-Venon Carlo Alessandria Alessandro Franchello Mauro Salizzoni Mario Rizzetto 《Liver transplantation》2005,11(4):402-409
Hepatitis B virus (HBV) recurrence after liver transplantation is significantly reduced by prophylaxis with hepatitis B immune globulins (HBIG) or antiviral drugs in nonreplicating patients and by the combination of both drugs in replicating patients. However, the load of HBV DNA, which defines replicating status in patients undergoing liver transplantation, remains unclear. This study analyzes the correlation between the viral load, tested with a single amplified assay, at the time of liver transplantation, and the risk of hepatitis B recurrence in 177 HBV carriers who underwent transplantation in a single center from 1990 to 2002. Overall, HBV relapsed after surgery in 15 patients (8.5%) with a 5- and 8-year actuarial rate of recurrence of 8% and 21%, respectively. After liver transplantation hepatitis B recurred in 9% of 98 selected subjects treated only with immune globulins and in 8% of 79 viremic patients who received immune globulins and lamivudine (P = NS). A linear correlation was observed between recurrence and viral load at the time of surgery. In transplant patients with HBV DNA higher than 100,000 copies/mL, 200-99,999 copies/mL, and DNA undetectable by amplified assay, hepatitis B recurred in 50%, 7.5%, and 0% of patients, respectively. Overall, a viral load higher than 100,000 copies/mL at the time of liver transplantation was significantly associated with hepatitis B recurrence (P = .0003). In conclusion, spontaneous or antiviral-induced HBV DNA viral load at the time of surgery classifies the risk of HBV recurrence after liver transplantation and indicates the best prophylaxis strategy. 相似文献