PURGE OF MALIGNANT CELLS FROM BONE MARROW BY HEMATOPORPHYRIN DERIVATIVES AND LIGHT EXPOSURE IN VITRO

During autologous bone marrow graft in treatment of malignant diseases, it is critical to purge malignant cells from the marrow. In the present study, the sensitivity to photodynamic inactivation of 3 leukemic cell lines was compared with their counterpart normal hematopoietic cells. After mouse leukemic L1210 cells were treated with a preparation of hematoporphyrin derivatives, YHpD, 10 μg/ml for 1 hr. and irradiated with blacklight (peak wavelength 395 nm, light intensity 0.6 mW/cm2) for 5 minutes, the survival rate of clonogenic cells decreased to <10%, while that of bone marrow granulocyte macrophage progenitor cells (CFU-GM) in DBA/2 mice remained at nearly normal level (>80%). Similar results were obtained when human leukemic HL-60 cells were compared with human CFU-GM and mouse leukemic L615 cells with CFU-GM in 615 strain mice. It is suggested that hematoporphyrin photoradiation may be useful for Iselectively killing leukemic cells in bone marrow.     

自体骨髓体外净化的研究进展

近年来用大剂量化疗联合自体骨髓移植(ABMT)治疗传统方法无效的急性白血病、慢性粒细胞白血病、实体瘤等已取得很大进展。但是急性白血病、慢性白血病、伴骨髓侵犯的淋巴瘤或实体瘤等,即使在血液学检查正常的完全缓解期中采取的骨髓也残留癌细胞,而这些残存的癌细胞是引起疾病复发的主要原因。因此如何有效地清除自体骨髓中残存的癌细胞就成为自体骨髓移植能否用于癌症根治的关键之一。目前国内外试探用于净化骨髓的方法主要有药物学方法、免疫学方法、物理学方法以及这些方法的联合应用。