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1.
HepG2 ceils were treated with various concentrations of tumor necrosis factor-or (TNF-α) for 24 hours. RT-PCR and Western blot were used to measure protein tyrosine phosphatase-1 B(PTP-1B)expression, and luciferase reporter assay was used to detect NF-kB activity. The results showed that treatment of HepG2 cells with TNF-α for 24 hours led to upregulation of PTP-1B and NF-kB activity in a dose-dependent manner. Inhibition of NF-kB by PI)TC significantly attenuated TNF-α-induced PTP-IB expression in HepG2 cells. Thus, the transactivation of NF-kB seems to play an important role in the expression of PTP-1B in HepG2 cells induced by TNF-α. 相似文献
2.
肝细胞癌端粒酶活性及hTERT mRNA表达与术后早期复发的关系 总被引:3,自引:0,他引:3
目的:研究肝细胞癌端粒酶活性及人端粒酶逆转录酶(hTERT)mRNA表达与肝细胞癌术后早期复发的关系。方法:采用ELISA—TRAP法检测60例肝癌组织及其癌旁组织端粒酶活性,RT—PCR法检测hTERT mRNA表达,5例正常肝脏组织作为对照。分析端粒酶活性及hTERT mRNA表达与临床病理之间的关系。结果:肝癌组织端粒酶活性及hTERT mRNA表达阳性率分别为86.7%(52/60)及90%(54/60),癌旁组织端粒酶活性及hTERT mRNA表达阳性率分别为40%(24/60)及43.3%(26/60)。正常肝脏组织均未检测到端粒酶活性及hTERT mRNA表达。癌旁组织端粒酶活性及hTERT mRNA表达与术后早期复发及包膜浸润、门静脉侵犯、肝内转移等恶性肿瘤的恶性生物学行为有关。结论:癌旁组织端粒酶活性及hTERT mRNA表达可能是肝细胞癌术后早期复发的预后指标。 相似文献
3.
HepG2 ceils were treated with various concentrations of tumor necrosis factor-or (TNF-α) for 24 hours. RT-PCR and Western blot were used to measure protein tyrosine phosphatase-1 B(PTP-1B)expression, and luciferase reporter assay was used to detect NF-kB activity. The results showed that treatment of HepG2 cells with TNF-α for 24 hours led to upregulation of PTP-1B and NF-kB activity in a dose-dependent manner. Inhibition of NF-kB by PI)TC significantly attenuated TNF-α-induced PTP-IB expression in HepG2 cells. Thus, the transactivation of NF-kB seems to play an important role in the expression of PTP-1B in HepG2 cells induced by TNF-α. 相似文献
4.
目的研究p53对二乙基亚硝胺(DEN)诱导肝细胞恶变过程的影响,探讨p53功能缺失与细胞恶变的关系。方法使用p53特异性抑制剂pifithrin-α(PFT-α)和(或)人p53小RNA干扰质粒sip53抑制L02细胞p53的转录活性。采用荧光素酶报告基因法检测不同时间点、不同浓度DEN对p53功能正常和缺失的L02细胞AP-1转录激活活性的影响,检测不同DEN处理时间对p53转录激活活性的影响及PFT-α对AP-1转录激活活性的影响。转录激活活性用相对荧光强度表示。结果应用PFT-α或转染sip53质粒24h后,p53的转录活性明显下降。单纯DEN处理后AP-1的相对荧光强度轻度上调,而DEN+PFT-α和DEN+sip53处理的细胞内AP-1相对荧光强度与单纯DEN处理组比较均有上调,呈现时间和浓度双重依赖性;在作用24h时点或DEN浓度为100ng/L时作用达高峰,随后回落。DEN处理后p53相对荧光强度明显上调,且存在时间依赖性,在24h达高峰,随后回落。DEN+PFT-α处理的细胞p53相对荧光强度在各时间点的变化不明显,始终保持在较低水平。以PFT-α抑制p53功能后,L02细胞内AP-1的相对荧... 相似文献
5.
HepG2 ceils were treated with various concentrations of tumor necrosis factor-or (TNF-α) for 24 hours. RT-PCR and Western blot were used to measure protein tyrosine phosphatase-1 B(PTP-1B)expression, and luciferase reporter assay was used to detect NF-kB activity. The results showed that treatment of HepG2 cells with TNF-α for 24 hours led to upregulation of PTP-1B and NF-kB activity in a dose-dependent manner. Inhibition of NF-kB by PI)TC significantly attenuated TNF-α-induced PTP-IB expression in HepG2 cells. Thus, the transactivation of NF-kB seems to play an important role in the expression of PTP-1B in HepG2 cells induced by TNF-α. 相似文献
6.
HepG2 ceils were treated with various concentrations of tumor necrosis factor-or (TNF-α) for 24 hours. RT-PCR and Western blot were used to measure protein tyrosine phosphatase-1 B(PTP-1B)expression, and luciferase reporter assay was used to detect NF-kB activity. The results showed that treatment of HepG2 cells with TNF-α for 24 hours led to upregulation of PTP-1B and NF-kB activity in a dose-dependent manner. Inhibition of NF-kB by PI)TC significantly attenuated TNF-α-induced PTP-IB expression in HepG2 cells. Thus, the transactivation of NF-kB seems to play an important role in the expression of PTP-1B in HepG2 cells induced by TNF-α. 相似文献
7.
CXCL16基因与红色荧光蛋白pDsRed2-N1融合基因表达载体的构建 总被引:1,自引:0,他引:1
目的:构建人趋化因子配体16(CXC ligand 16,CXCL16)与增强型红色荧光蛋白(red fluorescent protein,RFP)的融合基因表达载体pDsRed2-N1/CXCL16,使CXCL16-RFP融合蛋白在人293T细胞中稳定表达。方法:针对CXCL16基因设计引物,扩增人CXCL16 cDNA,退火后插入含有RFP的表达载体pDsRed2-N1。测序正确的CXCL16和RFP的融合基因表达载体pDsRed2-N1/CXCL16,经脂质体转染人293T细胞株,G418筛选获得稳定转染的细胞株。荧光显微镜观察细胞转染情况,Western blotting比较转染前后293T细胞中CXCL16蛋白的表达变化。结果:经转化细菌、抽提质粒、酶切鉴定和DNA序列分析证实,CXCL16基因已正确插入pDsRed2-N1中RFP基因的上游,获得融合基因表达载体pDsRed2-N1/CXCL16。空载体pDsRed2-N1和融合基因表达载体pDsRed2-N1/CXCL16经脂质体转染人293T细胞后,在荧光显微镜下可观察到表达RFP的细胞发出红色荧光,转染效率分别可达95%和85%。pDsRed2-N1/CXCL16转染293T细胞后,CXCL16蛋白的表达水平显著上调。结论:成功构建的pDsRed2-N1/CXCL16融合基因表达载体可在293T细胞中稳定表达CXCL16蛋白,为进一步研究CXCL16在细胞增殖调控中的作用奠定了基础。 相似文献
8.
HepG2 ceils were treated with various concentrations of tumor necrosis factor-or (TNF-α) for 24 hours. RT-PCR and Western blot were used to measure protein tyrosine phosphatase-1 B(PTP-1B)expression, and luciferase reporter assay was used to detect NF-kB activity. The results showed that treatment of HepG2 cells with TNF-α for 24 hours led to upregulation of PTP-1B and NF-kB activity in a dose-dependent manner. Inhibition of NF-kB by PI)TC significantly attenuated TNF-α-induced PTP-IB expression in HepG2 cells. Thus, the transactivation of NF-kB seems to play an important role in the expression of PTP-1B in HepG2 cells induced by TNF-α. 相似文献
9.
HepG2 ceils were treated with various concentrations of tumor necrosis factor-or (TNF-α) for 24 hours. RT-PCR and Western blot were used to measure protein tyrosine phosphatase-1 B(PTP-1B)expression, and luciferase reporter assay was used to detect NF-kB activity. The results showed that treatment of HepG2 cells with TNF-α for 24 hours led to upregulation of PTP-1B and NF-kB activity in a dose-dependent manner. Inhibition of NF-kB by PI)TC significantly attenuated TNF-α-induced PTP-IB expression in HepG2 cells. Thus, the transactivation of NF-kB seems to play an important role in the expression of PTP-1B in HepG2 cells induced by TNF-α. 相似文献
10.
HepG2 ceils were treated with various concentrations of tumor necrosis factor-or (TNF-α) for 24 hours. RT-PCR and Western blot were used to measure protein tyrosine phosphatase-1 B(PTP-1B)expression, and luciferase reporter assay was used to detect NF-kB activity. The results showed that treatment of HepG2 cells with TNF-α for 24 hours led to upregulation of PTP-1B and NF-kB activity in a dose-dependent manner. Inhibition of NF-kB by PI)TC significantly attenuated TNF-α-induced PTP-IB expression in HepG2 cells. Thus, the transactivation of NF-kB seems to play an important role in the expression of PTP-1B in HepG2 cells induced by TNF-α. 相似文献