人肺癌CEA单克隆抗体免疫组化研究

应用间接酶标法对人体不同组织类型的61例肺癌进行了CEA单克隆抗体的免疫组化研究,鳞癌阳性率为92％,腺癌86.7％。CEA在鳞癌呈明显的细胞异质性分布,在同一癌巢中,仅位于癌巢中心、分化较好的细胞CEA阳性。CEA在鳞癌呈膜性分布;腺癌为胞浆分布。尽管鳞癌和腺癌CEA阳性率较高,但对其应用于示踪导向疗法的价值尚有待探讨。     

人肺肿瘤瘤复合糖类和CEA—糖蛋白组化定位研究

Sixty-six human lung neoplasms of different histological types and normal bronchial epithelial cells of newborn babies and adults were studied histochemically using ConA and PSA and the result was compared with that of CEA. Normal mucosal epithelium could bind to ConA, and the location of ConA receptors was related to the maturation of mucosal epithelial cells. Normal mucosal epithelium in adult bronchi failed to be stained with PSA and anti-CEA, and most of lung neoplasms could bind to PSA and positive for CEA, indicating that new glycoconjugate and CEA-glycoprotein could be synthesized after malignant transformation of mucosal epithelium. The binding of ConA, PSA and anti-CEA to cell membrane and nucleus membrane was characteristic of squamous cell lung cancer while lung adenocarcinoma mainly showed cytoplasmic staining. The weak staining of ConA, PSA and anti-CEA in small cell carcinoma and negative staining in carcinoid and malignant melanoma help testify that their origin may differ from that of squamous cell carcinoma and adenocarcinoma.     

伴有肺广泛出血病变的Wegener’s肉芽肿病(附一例尸检报告)

作者对1例wegener’s肉芽肿病的尸体解剖基本病变进行了研究,结果显示除具有呼吸系统的肉芽肿病损、全身性血管炎及肾小球炎的典型病变外,肺弥漫性出血、肺泡隔坏死性毛细血管炎以及肝、肾等多个器官的坏死性血管炎及广泛组织纤维化较显著。     

AN IMMUNOHISTOCHEMICAL STUDY OF OCCULT MICRO- METASTASES IN REGIONAL LYMPH NODES OF 94 PATIENTS WITH STAGE I NON-SMALL CELL LUNG CARCINOMA

In the study, 739 regional lymph nodes from 94 patients with stage I non- small cell lung carcinoma (NSCLC) were studied by immunohistochemical techniques. These lymph nodes contained no metastatic tumor as assessed by conventional histopatholgy were recut. A series of consecutive sections from the original blocks were immunostained with poly-and monoclonal antibodies to cytokeratins, carcinoembryonic antigen (CEA), and human milk fat globulin membrane antigen (HMFG-2). Single tumor cells or small clusters of tumor cells, not visible on routine examination, were readily detected. The actual number of lymph nodes that contained occult tumor cells was 123 (16.6%) from 53 patients (56.4%). The majority of 102 immunostalned positive nodes were distributed in the hllar (29% ) and peribronchlal (25%) regions. Our data indicate that (1) a series of consecutive sections and immunohistochemistry may greatly Increase the diagnostic yield of occult micrometastases in lymph nodes; (2) the high incidence of occult m     

子宫颈上皮细胞癌变过程中细胞复合糖类变化研究

作者应用凝集素PNA、PSA、BSL观察了新疆妇女宫颈上皮细胞癌变过程中细胞复合糖类的变化。PNA受体的表达与宫颈上皮细胞的分化关系密切。正常宫颈上皮基底细胞阳性,棘层和表层细胞PNA受体阴性,去分化后有丰富的PNA受体表达。宫颈上皮细胞PNA受体的出现可被认为是上皮细胞去分化的标志。PSA受体在宫颈上皮细胞癌变过程中呈递增趋势,然而PSA受体与宫颈上皮细胞的分化状态关系不大。