Effects of basic fibroblast growth factor on biological characteristics of osteoblasts

Objective: To elucidate the effects of exogenous basic fibroblast growth factor ( bFGF ) on biological characteristics of rat osteoblasts cultured in vitro. Methods: The osteoblasts isolated from a Sprague-Dawley rat and cultured in vitro were treated with different concentrations of bFGF ( 5-50 ng/ml) respectively. At 24 hours after treatment, the proliferating cell nuclear antigen was measured with immunocytochemistry, alkaline phosphatase (ALP) activity was determined and the expression of transforming growth factor beta 1 ( TGF-β1 )was detected to observe the effects of bFGF on growth and differentiation of osteoblasts. Resu/ts: bFGF ( 5-50 ng/ml ) could obviously promote the growth of osteoblasts. The intracellular expression of TGF-β1 mRNA increased significantly, but the intracellular ALP content decreased. Conclusions: bFGF can obviously stimulate the proliferation of osteoblasts and promote the synthesis of TGF-β1, but cannot promote the differentiation of osteoblasts.     

多发性骨折后全身炎症反应综合征的监控

目的　分析多发性骨折后全身炎症反应综合征 (SIRS)的监测因素。方法　将 87例多发性骨折患者分为SIRS组、非SIRS组和死亡组 ,比较各组血清肿瘤坏死因子 (TNF -α)、粒细胞集落刺激因子 (G -CSF)、补体C3 、免疫球蛋白 (IgG)等的变化。同时观察失血量分级与SIRS的关系。结果　SIRS组G -CSF和IgG水平明显低于非SIRS组 ,TNF -α水平明显高于非SIRS组 (P <0 .0 5 ) ;死亡组G -CSF、补体C3 及IgG水平较SIRS组进一步降低 ,TNF -α水平进一步升高 (P <0 .0 5 )。随失血量增大 ,SIRS发生率、病死率升高 (P <0 .0 5 )。结论　G -CSF ,IgG ,TNF -α水平及失血量可作为SIRS的监控指标。     

Abmessung und Analyse der Bezugsflche von Acetabulum-Prothese-Transplantationin unterschiedlichen Krperlagen

Bei der Permutationsoperation des Pfannenge-lenks ist die Genauigkeit des Transplantation-swinkels der H櫣ftpfannen-Prothese ein wichtigerFaktor bez櫣glich der Auswirkung der Operation.Schlechte Positionierung der H櫣ftpfannenprothesekann zu Komplikationen wie Luxation nach Opera-tion,starke Verschleiss der Prothese,Lockerungder Prothese usw.f櫣hren[1,2].Bei der Transplan-tation der H櫣ftpfannenprothese wird meistens dieFlache zwischen Ant.Sup.Iliac spine auf beidenSeiten und Pubic t…     

Ultrasonographic Evaluation of Osteosarcomas

The ultrasonographic appearances of osteosarcomas and the roles of ultrasonography in the diagnosis and surgical staging of osteosarcomas were investigated. A comparative study was performed on 45 cases of osteosarcomas by ultrasonography and radiography. Bony changes, periosteal reaction and soft tissue mass were evaluated for each lesion. The results showed that ultrasonography revealed a solid mass around bone in 42 patients, bone destruction in 24 patients and periosteal reaction in 16 patients. Plain radiographs showed bony changes in 44 patients and no bony change in remaining one patient, shadowing of soft tissue swelling in 30 patients, and pulmonary metastases in 3 patients. Surgical biopsy and pathological examination confirmed osteosarcoma in all 45 patients. Soft tissue mass was confirmed in 42 patients surgically. The diagnostic accuracy of soft tissue masses by ultrasonography and radiography was 100 % (42/42) and 71.4 % (30/42), respectively. The positive rate of ultrasonography and radiography in displaying bony changes was 53.3 % (24/45) and 97.8 % (44/45), respectively. In conclusion, in the detection of soft tissue mass of osteosarcoma, ultrasonography is superior to radiography, and in displaying bony changes of osteosarcomas, radiography is superior to ultrasonography. So it may come to a conclusion that plain radiography combined with ultrasonography can completely display the bony and soft tissue lesion of osteosarcomas.     

骨髓细胞检查在贫血病因诊断中的临床价值

目的研究分析骨髓细胞检查在诊断贫血病因诊断方面的临床价值。方法选择2011年3月~2014年3月在我院收治的200例贫血患者,对其行骨髓的细胞学检查,并对检查结果进行详细分析以判断其病因。结果有179例(89.5%)患者被诊断出分别患有12种以上的疾病,但仍有21例(10.5%)患者的疾病原因不能被诊断出。结论目前临床的骨髓细胞学检查为贫血患者的病因诊断提供了重要且可靠性的依据,但仍有一定的局限性存在。     

艾滋病合并肺结核35例CT征象分析

目的 分析艾滋病合并肺结核35例患者的临床资料及CT征象分析,总结其CT影像特点,提高对艾滋病合并肺结核的影像诊断水平.方法 收集35例艾滋病合并肺结核患者的临床资料及CT征象,分析和探讨艾滋病合并肺结核的CT特点.结果 艾滋病合并肺结核患者,其临床症状重,但胸部影像表现多不典型:(1)双肺不典型血播多见,表现为双肺弥漫粟粒结节影并广泛间质增厚,部分融合成小片状.(2)1～2个肺段的片团状实变多见,出现干酪坏死少见.(3)双肺毛玻璃影多见.(4)合并多浆膜腔积液(双侧胸腔积液及心包积液)多见.(5)空洞少见.结论 艾滋病合并肺结核的临床表现不典型,CT表现有一定特点,早期临床医生应该高度重视,提高其诊断率.     

广泛性焦虑症二重辨证施治在开放式心理病房的研究

目的　探讨二重辨证施治对广泛性焦虑症的疗效 ,及其在开放式心理病房的适应情况。方法　将 73例广泛性焦虑症患者按随机的原则在开放式心理病房分为研究组 (36例 )和对照组 (37例 )。研究组用二重辨证施治 ,对照组用认知疗法结合阿普唑仑、氟西汀等。研究时间共 5个月 ,前 2个月为积极治疗期 ,后 3个月为自由治疗期。采用临床疗效评定标准 ,贝克焦虑量表 (BAI) ,χ2 检验 ,t检验。结果　研究组和对照组 ,积极治疗期末痊愈差异有统计学意义 (χ2 =4 .39,0 .0 5 >P>0 .0 2 5 ) ,自由治疗期末痊愈差异有更大的统计学意义 (χ2 =13.0 8,P <0 .0 0 5 )。贝克焦虑量表 (BAI)标准总分 ,两组治疗前差异无统计学意义 ;积极治疗期末 ,两组差异有统计学意义 (0 .0 0 2

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