检测血管生长因子作用的鸡胚绒毛尿囊膜技术

采用改良的鸡胚绒毛尿囊膜技术，探讨血管生长因子在肿瘤发生发展过程中的作用。方法是将肿瘤细胞培养上清液或某些细胞因子，置于孵育９ｄ鸡胚的ＣＡＭ膜上，继续孵育３ｄ后取膜，观察新生毛细血管的数量、长度和粗细，发现恶性程度高的肿瘤培养上清液促血管生长作用较强。     

一个具有肿瘤转移抑制基因活性的人DNA序列

目的 分离鉴定人类肿瘤转移抑制基因或相应ＤＮＡ序列，探讨转移表型逆转的分子生物学机制。方法 应用Ｉｎｔｅｒ Ａｌｕ ＰＣＲ技术，将导入正常人肺基因组ＤＮＡ片段后转移表型抑制细胞株中的人源性ＤＮＡ片段扩增出来，对其中的７００ｂｐ左右ＤＮＡ片段进行核苷酸序列分析，并在ＧｅｎＢａｎｋ序列数据库进行类似性检索。将该ＤＮＡ再次转染高转移小鼠瘤细胞株，通过体内、体外转移相关性实验，验证其是否具有抑制高转移小鼠     

TGFβ_1质粒DNA直接瘤内注射对小鼠肿瘤生长的影响

探讨裸ＤＮＡ质粒ＰＭＡＭｎｅｏ－ＴＧＦβ１小鼠瘤内直接注射后的表达及其对肿瘤生长的影响。方法肺腺癌细胞株ＬＭ３小鼠背部皮下接种，两周后成瘤。瘤体内多点多次直接注入质粒ＤＮＡＰＭＡＭｎｅｏＴＧＦβ１，并设空质粒和生理盐水注射组为对照，观察肿瘤生长情况。于第８周将小鼠处死，取新鲜的瘤组织提取ＲＮＡ，行Ｎｏｒｔｈｅｒｎ杂交，并病理制片观察肿瘤组织结构的变化。结果ＴＧＦβ１基因治疗组肿瘤生长速度增快，但各组间肿瘤转移的差异无显著性。Ｎｏｒｔｈｅｒｎ杂交证实，注入到瘤体内的ＴＧＦβ１基因获得了高效表达。结论ＴＧＦβ１基因的瘤内表达可能通过某种机制刺激肿瘤的生长；裸ＤＮＡ质粒直接瘤内注射法作为ｉｎｖｉｖｏ基因转移手段有其不可低估的应用前景。     

蛙皮素制备的抗人小细胞肺癌单克隆抗体

蛙皮素制备的抗人小细胞肺癌单克隆抗体付生法张朝山赵林普郭文君＊军事医学科学院基础医学研究所北京１００８５０文献报道肺癌的发生率有逐年增高的趋势，尤其是小细胞肺癌，其瘤细胞增殖快、易转移，因而对其早期诊断十分重要〔１〕。瘤细胞单克隆抗体是肿瘤的标志物，...     

THE EFFECT OF TGFb1 ON MURINE TUMOR GROWTH FOLLOWING DIRECT INTRATUMORAL INJECTION OF PLASMID DNA

ＴＨＥＥＦＦＥＣＴＯＦＴＧＦｂ１ＯＮＭＵＲＩＮＥＴＵＭＯＲＧＲＯＷＴＨＦＯＬＬＯＷＩＮＧＤＩＲＥＣＴＩＮＴＲＡＴＵＭＯＲＡＬＩＮＪＥＣＴＩＯＮＯＦＰＬＡＳＭＩＤＤＮＡＧａｏＰｉｎｇ１高平ＬｕＹｉｎｇｌｉｎ２陆应麟ＧｅＸｕｅｍｉｎｇ２葛学铭ＦａｎＷｅ...     

人克隆化LAK细胞对裸鼠人肺巨细胞癌细胞系模型的实验治疗

应用肿瘤病人外周血克隆化LAK细胞对裸鼠的人肺巨细胞癌细胞系高转移株(PLA801-D)模型进行实验治疗。结果表明,LAK细胞治疗组裸鼠14只平均活存天数118.3天,对照组12只平均活存88.8天,治疗组长于对照组,P<0.05。经LAK细胞治疗组有两例荷瘤裸鼠肿瘤消退。组织病理学观察,肿瘤细胞通过血行和淋巴转移于肺部和淋巴结,11例治疗组裸鼠中仅1例转移,而对照组裸鼠共观察8例中有4例发生转移,且1例除肺和淋巴结有转移外在心肌纤维间有广泛的肿瘤细胞转移。电镜观察,肿瘤细胞有超微结构的特点,部分细胞呈现凋亡的改变。本文对LAK细胞治疗的可能有效机理进行了初步探讨。     

质粒pUDKH刺激鸡胚绒膜尿囊膜血管生成效应的研究

目的 :探讨携带人肝细胞生长因子基因的质粒pUDKH对 13日龄鸡胚绒膜尿囊膜血管生成的刺激效应。方法 :以甲基纤维素为载体 ,将不同剂量的pUDKH(1.0 ,2 .0 ,5 .0 μg)或空白质粒pUDK(5 .0 μg)置入 13日龄鸡胚绒膜尿囊膜上 ,于孵化箱继续孵育 3d后 ,观察鸡胚绒膜尿囊膜上小血管的密度。结果 :应用质粒pUDKH各组小血管的密度均明显较对照组大 (P <0 .0 5 ) ,且有剂量_效应关系。结论 :质粒pUDKH可显著刺激鸡胚绒膜尿囊膜上小血管的形成 ,故认为其具有在体内应用于治疗缺血性疾病的潜能     

A novel human DNA sequence with tumor metastasis suppressive activity

　Objective To isolate human tumor metastasis suppressive DNA sequence and to study the molecular mechanisms regulating tumor metastasis. Methods A mouse lung adenocarcinoma cell clone 12 derived from its parent cell line LM2, which had been transduced with normal human genomic DNA, was previously reported. Compared with LM2, the metastatic potential of clone 12 was very much decreased. Clone 12 was used in this study to amplify the human DNA fragments by Inter Alu PCR technique. The human DNA fragments obtained were then transfected into LM2 cells and their malignant phenotype was tested in vitro and in vivo, and compared with that of the untransfected LM2 cells. Results Three human DNA fragments of 700, 500 and 300 bp were isolated. DNA sequencing revealed that the 700bp fragment does not show homology with hitherto reported genes and was accepted by the Genbank (pt712 U67835). In vitro proliferation and colony formation in soft agar of the 700 bp fragment-transfected LM2 cells were significantly inhibited as compared to the untransfected LM2 cells. Upon subcutaneous inoculation to syngeneic T739 mice, the 700bp–transfected LM2 cells grew more slowly and smaller tumors developed compared to the untransfected ones. Moreover, lung metastasis was not found in 6 of 10 mice inoculated with the 700bp-transfected LM2 cells, while it was found in 9 of 10 mice inoculated with the untransfected LM2 cells. The difference was statistically significant (P<0.001). The frequency of lymph node metastasis was also statistically different between the 2 groups of mice. Conclusion The newly isolated 700bp human DNA fragment may be a metastasis suppressor gene of malignant tumor.