驱白艾力勒思亚散乙醇提取物对小鼠B16黑素瘤细胞增殖及黑素生成的影响

目的研究维药复方驱白艾力勒思亚散(QBALLSYS)乙醇提取物对小鼠B16黑素瘤细胞增殖、黑素合成以及酪氨酸酶活性的影响。方法以MTT法测定培养的B16黑素瘤细胞的增殖活性;氢氧化钠法测定黑素生成量;体外氧化多巴反应方法测定酪氨酸酶活性。结果与空白对照组相比,驱白艾力勒思亚散乙醇提取物对B16黑素瘤细胞的增殖活性、黑素生成量、酪氨酸酶活性均有显著性作用(P<0.05或P<0.01)。驱白艾力勒思亚散乙醇提取物对小鼠B16黑素瘤细胞的增殖活性有上调作用,对细胞黑素生成以及酪氨酸酶的活性均有不同程度的促进作用。结论驱白艾力勒思亚散的乙醇提取物具有促进黑素细胞增殖和合成黑素的作用。     

比色法测定巴天酸模水提物中蒽醌类成分的含量

目的 测定巴天酸模水提取物中蒽醌类成分的含量.方法 运用比色法,以1,8-二羟基蒽醌为对照品,用0.8％乙酸镁甲醇溶液显色;在510 nm波长处测定巴天酸模水提取物中蒽醌类成分含量.结果 巴天酸模水提取物中蒽醌类成分含量达到31.07 mg/g,以结合型蒽醌为主.结论 比色法简便、精确、重现性好,可以作为巴天酸模水提取物中蒽醌类成分的含量测定方法.     

没食子药材HPLC指纹图谱研究

目的研究10个产地没食子药材的高效液相色谱指纹图谱,为科学评价和有效控制其质量提供可靠方法。方法采用Waters Symmetry ShieldTM RP C18(250 mm×4.6 mm,5μm)色谱柱,甲醇-0.2%磷酸水溶液为流动相,梯度洗脱,柱温30℃,体积流量1 mL/min,检测波长270 nm,进样量为20μL。结果对所采集的没食子药材进行测定,建立了没食子药材的指纹图谱,有7个共有峰,有2批药材的相似度低于0.9,与其他批次样品差异较大;此HPLC方法的精密度、稳定性和重现性良好。结论此方法准确、可靠、可控性强,可用于没食子药材的质量控制。     

湿疹的维吾尔医证候分类及其用药规律分析

目的:整理分析湿疹的维吾尔医证候分类及其用药规律。方法:采用回顾性整理分析方法,收集新疆6家维吾尔医医院5年内维吾尔医药治疗湿疹的临床住院病历,整理分析湿疹的维吾尔医证候分类及其治疗原则、方法、方药及其用药情况,探索湿疹的维吾尔医辨证施治方法及其规律。结果:符合纳入标准的湿疹住院病历621例,其中异常黏液质型患者327例(52.7%),异常胆液质为239例(38.5%),异常血液质33例(5.3%)和异常黑胆质型患者22例(3.6%)。采用成熟或调理及清除疗法,内服药物疗法,外用药物疗法和非药物疗法等综合治疗方法进行治疗。痊愈61例(9.8%),显效484例(77.9%),有效60例(9.7%),无效16例(2.6%)。结论:湿疹的维吾尔医证候分类及用药规律符合临床辨证实际,为其维吾尔医辨证施治的规范化提供了客观依据。     

白癜风证候分类及维吾尔医用药规律分析

目的:整理分析维吾尔医辨证施治白癜风的证候分类及维吾尔药用药规律。方法:采用回顾性整理分析方法,收集整理新疆5家维吾尔医医院10年内维吾尔医药治疗白癜风的临床住院病历,整理分析白癜风的维吾尔医证候分类与治疗原则、方法、方药及其用药情况,挖掘维吾尔医辨证施治白癜风的证候分类及用药规律。结果:符合纳入标准的异常黏液质型白癜风病历362例,其中无味黏液质173例(47.79%),石灰样黏液质46例(12.71%),酸味黏液质43例(11.88%),甜味黏液质40例(11.05%),涩味黏液质36例(9.94%),咸味黏液质24例(6.63%)。采用成熟及清除疗法,内服药物疗法,外用药物疗法和非药物疗法等综合治疗方法进行治疗。结论:白癜风的维吾尔医证候分类及用药规律符合临床辨证实际,为其维吾尔医辨证施治的规范化提供了客观依据。     

没食子提取物小鼠急性毒性实验研究

目的初步了解没食子提取物对小鼠急性毒性的强弱及特点,测定急性毒性的相关参数。方法小鼠观察检疫3 d,随机分为6组:空白对照组、没食子提取物组:3.25,4.25,5.75,7.5,10.0 g/kg.bw剂量组,灌胃给予没食子提取物,观察其毒性表现。结果没食子提取物组随着剂量的增加,毒性反应表现越明显,小鼠死亡只数也随之增加;空白对照组动物均无异常。结论没食子提取物小鼠口服急毒LD50为4.41 g/kg;没食子提取物可能对胃肠系统有一定的毒性作用。     

地锦草抗真菌有效部位中芦丁及槲皮苷含量的高效液相色谱法测定

目的 建立高效液相色谱(HPLC)法同时测定维吾尔医药地锦草抗真菌有效部位中芦丁及槲皮苷含量的方法.方法 色谱柱为waters symmetry C18(4.6 mm×150 mm,5μm);流动相为甲醇-0.4％磷酸水溶液(42:58,V/V),,检测波长为360nm;流速为1.0 ml· min-1;柱温25℃;进样量10μl.结果 芦丁和槲皮苷线性范围分别在0.42～2.52 μg,0.6～3.6μg内线性关系良好(r≥0.999 5),平均加样回收率分别为99.2％,101.1％,RSD≤3.3％.结论 该方法操作简便、准确、灵敏度高、重复性好,可用于地锦草抗真菌有效部位的质量控制.     

一枝蒿提取物对小鼠胃肠运动的影响

目的观察一枝蒿水提物对小鼠胃肠运动的影响,探讨其作用机制。方法将小鼠随机分为正常对照组、多潘立酮组及一枝蒿水提物高、中、低剂量组。灌胃给药5d后,以葡聚糖蓝2000为标记物,观察色素在5组小鼠胃内的残留比,以及幽门括约肌至色素最前端及至盲肠的距离比。结果一枝蒿高剂量组及多潘立酮组的胃残留率、肠推进率与对照组比较,差异有统计学意义（P〈0．05,P〈0．01）,2组之间差异无统计学意义（P〉0．05）。结论一枝蒿水提物能有效改善小鼠的胃肠动力。     

Antifungal Activity of Gallic Acid In Vitro and In Vivo

Gallic acid (GA) is a polyphenol natural compound found in many medicinal plant species, including pomegranate rind (Punica granatum L.), and has been shown to have antiinflammatory and antibacterial properties. Pomegranate rind is used to treat bacterial and fungal pathogens in Uyghur and other systems of traditional medicine, but, surprisingly, the effects of GA on antifungal activity have not yet been reported. In this study, we aimed to investigate the inhibitory effects of GA on fungal strains both in vitro and in vivo. The minimal inhibitory concentration (MIC) was determined by the NCCLS (M38‐A and M27‐A2) standard method in vitro, and GA was found to have a broad spectrum of antifungal activity, with MICs for all the tested dermatophyte strains between 43.75 and 83.33 μg/mL. Gallic acid was also active against three Candida strains, with MICs between 12.5 and 100.0 μg/mL. The most sensitive Candida species was Candida albicans (MIC = 12.5 μg/mL), and the most sensitive filamentous species was Trichophyton rubrum (MIC = 43.75 μg/mL), which was comparable in potency to the control, fluconazole. The mechanism of action was investigated for inhibition of ergosterol biosynthesis using an HPLC‐based assay and an enzyme linked immunosorbent assay. Gallic acid reduced the activity of sterol 14α‐demethylase P450 (CYP51) and squalene epoxidase in the T. rubrum membrane, respectively. In vivo model demonstrated that intraperitoneal injection administration of GA (80 mg/kg d) significantly enhanced the cure rate in a mice infection model of systemic fungal infection. Overall, our results confirm the antifungal effects of GA and suggest a mechanism of action, suggesting that GA has the potential to be developed further as a natural antifungal agent for clinical use. Copyright © 2017 John Wiley & Sons, Ltd.     

Active Achilles tendon kinesitherapy accelerates Achilles tendon repair by promoting neurite regeneration

Active Achilles tendon kinesitherapy facilitates the functional recovery of a ruptured Achilles tendon. However, protein expression during the healing process remains a controversial issue. New Zealand rabbits, aged 14 weeks, underwent tenotomy followed immediately by Achilles tendon microsurgery to repair the Achilles tendon rupture. The tendon was then immobilized or subjected to postoperative early motion treatment (kinesitherapy). Mass spectrography results showed that after 14 days of motion treatment, 18 protein spots were differentially expressed, among which, 12 were up-regulated, consisting of gelsolin isoform b and neurite growth-related protein collapsing response mediator protein 2. Western blot analysis showed that gelsolin isoform b was up-regulated at days 7-21 of motion treatment. These findings suggest that active Achilles tendon kinesitherapy promotes the neurite regeneration of a ruptured Achilles tendon and gelsolin isoform b can be used as a biomarker for Achilles tendon healing after kinesitherapy.