Prokinetic and laxative activities of Lepidium sativum seed extract with species and tissue selective gut stimulatory actions

Aim of the study

To provide ethnopharmacological basis for the medicinal use of Lepidium sativum seeds in indigestion and constipation.

Materials and methods

The in vivo studies were conducted in mice, while isolated tissues of mouse, guinea-pig and rabbit were suspended in tissue bath to measure isotonic contractions.

Results

The aqueous-methanolic extract of Lepidium sativum seeds (Ls.Cr) at 30 and 100 mg/kg showed atropine-sensitive prokinetic and laxative activities in mice, which were partially sensitive to atropine. In isolated gut preparations of mouse and guinea-pig, Ls.Cr (0.1-1 mg/mL) caused a concentration-dependent stimulatory effects both in jejunum and ileum, which was blocked in the presence of atropine. In rabbit jejunum, the stimulant effect of Ls.Cr remained unchanged in the presence of atropine, pyrilamine or SB203186, while in rabbit ileum, the stimulatory effect was partially blocked by atropine. The Ls.Cr was more efficacious in gut preparations of rabbit than in guinea-pig or mouse. The phytochemical analysis of the plant extract detected alkaloids, saponins and anthraquinones as plant constituents.

Conclusion

This study showed the prokinetic and laxative effects of Lepidium sativum in mice, which were partially mediated through a cholinergic pathway. The in vitro spasmodic effect of the plant extract mediated through a similar mechanism with species and tissue-selectivity, provides a rationale for the medicinal use of the seeds of Lepidium sativum in indigestion and constipation, and suggests studying the plant extracts on more than one species to get the wider picture.     

An inventory of the ethnoveterinary practices for reproductive disorders in cattle and buffaloes, Sargodha district of Pakistan

AIM OF THE STUDY: This study was conducted to document the ethnoveterinary practices (EVPs) used for the treatment and/or management of reproductive disorders of cattle and buffaloes. MATERIALS AND METHODS: The information was collected using rapid and participatory rural appraisal techniques through interviews and focused group discussions with 217 traditional veterinary healers (TVHs) over a period of 16 months. RESULTS: The use of 66 plant species was documented for the treatment of reproductive disorders in the two dairy species. Most frequently reported (>or=20 times) plants represented Apiaceae, Poaceae, Pedaliaceae, Linaceae, Arecaceae, Brassicaceae, Malvaceae, Zingiberaceae, Rosaceae, Cannabaceae, Fabaceae, Moraceae, Rubiaceae and Mimosaceae families. All the documented plant species were indigenous to the study area. Genital prolapse, retention of fetal membranes, silent estrous/delayed puberty, anestrous, dystokia associated with incomplete cervical dilatation and retention of lochial discharge were the frequently reported reproductive disorders. Some of the plants were reported to be used in more than one condition. Materials other than plants used for the treatment of these disorders included camel milk, butter and butter fat, minerals, eggs, organic matters, chemical substances and meat preparations. These materials were used either alone or as adjunctive therapy. CONCLUSION: Richness of EVPs in the study area and extensive variation in the doses, methods of preparation, indications, and claims regarding efficacy of plants for various disorders merit controlled studies for their validation.