粒细胞、肺泡巨噬细胞在烟雾吸入伤发病中的作用及粉防己碱减轻肺损伤的实验研究

烟雾吸人性损伤(Smoke Inhalation Injury)是烧伤的重要死亡原因,病人主要死于气道阻塞和早期肺水肿引起的呼吸衰竭。目前,对其发病机理尚未完全了解,因而缺乏有效的防治措施。本研究的目的在于进一步探讨炎症细胞与吸入伤发病的关系及粉防已碱的疗效和作用机制。 材料与方法 采用本所建立的家兔重度烟雾吸入伤模型致伤,将72只家兔随机分成正常对照组(NC组,     

Effects of Ginsenosides and Ketoprofen on Smoke Inhalation Injury in Rabbits and Rats

Six hours after smoke inhalation injury in rabbits, the permeability of pulmonary vesselsand the aggregation of circulating platelets increased markedly accompanied with apparent patholog-ical changes in the trachea and lungs. Fifteen minutes after smoke inhalation injury in rabbits, an intravenous dose of ginsenosides or ketoprofenwas given to the animals respectively. 6 hours after medication, it was found that both the drugscould significantly alleviate the platelet aggregation, but only ginsenosides could alleviate theaugmentation of pulmonary vascular permeability and the pathological lesions in the trachea andlungs. In those rats injured by smoke inhalation, l hour after an intravenous dose of ginsenosides, theplasma PGI_2 level was elevated and TXA_2/PGI_2 ratio decreased significantly.     

粉防己碱对炎症白细胞第二信使物质的作用

目的:研究粉防己碱(Tet)对炎症白细胞第二信使物质cAMP、[Ca2 ]i及IP3的作用。方法:收集大鼠角叉菜胶性胸膜炎渗出液中炎症白细胞,采用竞争性蛋白结合分析、放射免疫分析、酶放射化学分析和荧光比色法,分别测定Tet对炎症白细胞cAMP生成     

医疗设备维修管理

医疗设备维修工程师的人员合理安排，技术档案的建立，监督管理体系的健全和维修方法的介绍。     

腹腔镜Smith—Nephew气腹机故障分析

九十年代后 ,腔镜手术被广泛应用于各大医院临床 ,特别是腹腔镜手术在肝胆及盆腔疾病中具有手术时间短、手术创面小、愈合快等优点。目前 ,世界上比较知名的品牌有日本的奥林巴斯 ,美国的史赛克、Ｓｍｉｔｈ -Ｎｅｐｈｅｗ、德国的ＷＯＬＦ及欧洲的一些品牌如Ｓｔｏｒｚ等等。腹腔镜手术设备主要包括气腹机、数码摄像监视系统及冷光源三部分。另外还需手术冲洗器、罐装ＣＯ2 气体及连接管作为外围设备使用。气腹机是腹腔镜手术中必不可少的仪器 ,其作用主要是在病人的腹腔内充入纯ＣＯ2 气体使腹腔内有足够的空间容纳手术器械在里面进行手术…     

Effects of tetrandrine on the changes of free calcium level in neutrophils after smoke inhalation injury in the rabbit

The changes of the cytoplasmic free calcium level in the neutrophils after smoke in-halation injury were observed in rabbits and then the effects of tetrandrine,a calcium antago-nist,on the changes of free calcium level were studied.It was found that the number of neu-trophils increased significantly preceded by a transient decrease in the blood and also increasedin the bronehoalveolar lavage fluid after smoke inhalation.and the level of cytoplasmic free calci-um in the blood neutrophil increased likewise.Administration of tetrandrine resulted in a reduc-tion of the neutrophils number in the lungs and the free calcium level in the blood neutrophils toalleviate the pulmonary injury due to smoke inhalation.It is believed that there is a close rela-tionship between the activation of neutrophils and the pathophysiological changes of the lungs,and tetrandrine can exert its therapeutic effects on the injury by decreasing the free calcium levelin the neutrophils to modulate their functions.     

粉防已碱抗炎作用机理的研究

粉防已碱(Tetrandrine,Tet)是从中药粉防已中提出的有效成分,单体为双苄基异喹啉衍生物,具有镇痛,消炎,抗风湿,抗过敏,抗心律失常,降压等作用,临床上已用于高血压的治疗,虽然五十年代吕富华等已证明Tet有治疗甲醛性关节炎的作用,但因条件限制,至今对其作用机制,尤其是抗炎作用机制仍不明确。为此。我们进行了一系列研究,目     

人参总皂甙对血小板聚集和环核苷酸代谢的影响

为了探讨人参总皂甙(Gensenosides GS)对血小板的作用及其机理,本文观察了GS对兔血小板聚集和环核苷酸代谢的影响。结果表明,GS(12mg/kg)由兔耳缘静脉给药后,曲花生四烯酸,ADP及胶胶诱导的血小板聚集率比给药前明显降低,同时测得血小板中cAMP含量比给药前明显升高,两个指标的时效曲线均大致呈双峰型,高