逆行荧光标记观察猫上橄榄复合体向内耳的投射

Objective To investigate the distribution and projective feature of cat olivocochlear neurons. Methods Eleven adult cats were divided into two groups randomly. The experimental group of eight cats was injected of I% cholera toxin B (CTB) to the left cochlea, while injected of 5% fluoro gold (FG) to the right cochlea. The control group of three cats was injected of saline to bilateral cochlea. After a survival time of 7 days, serial frozen sections were cut in the cat brainstem. All the sections were processed by immunofluoreseent procedure for CTB and FG, and the labeled olivocochlear neurons were observed byfluorescent microscope. Results In the experimental group, the mean total of olivocochlear neurons labeled by CTB and FG was 3210 ± 168, including lateral olivocochlear neurons (LOC, 2298 ± 120) and medial olivocochlear neurons (MOC,913 ± 64). The labeled neurons were divided into three different types according to their feature of projection: neurons which only projected to the ipsilateral cochlea, neurons which only projected to the contralateral cochlea, and double-labeled neurons which projected both to the ipsilateral and contralateral cochlea, but the double-labeled neurons comprised 3.9% and 15. 1% in the LOC and MOC system respectively. No labeled neurons were found in the control group. Conclusions There are three types of neurons in the cat olivocochlear system. The neurons which projected to the bilateral cochlea may distribute both in the LOC and MOC system.     

缺血性结肠炎患者的血黏度及血脂分析

目的：分析缺血性结肠炎（IC）患者的血黏度及血脂状况，为防治IC提供依据。方法确诊为IC患者（观察组）与健康者（对照组）各43例，测定高切变率下的全血黏度、低切变率下的全血黏度、D-二聚体、总胆固醇（CHOL）、三酰甘油（TG）、高密度脂蛋白胆固醇（HDL-C）、低密度脂蛋白胆固醇（LDL-C）、载脂蛋白A1（Apo-A1）、载脂蛋白B（Apo-B）。结果观察组高切变率全血黏度、低切变率全血黏度、D-二聚体、CHOL、TG、LDL-C、Apo-B 水平，均高于对照组，HDL-C、Apo-A1低于对照组，差异均有统计学意义。结论缺血性结肠炎患者血黏度及血脂明显异常，血管栓塞风险高，积极改善微循环与合理的调脂治疗，可预防和减缓缺血性结肠炎的发生及进展。     

大黄在肛肠疾病中的临床应用

大黄药用历史悠久,自《神农本草经》始载至今,历代医家都把大黄作为治疗热毒疮疡之要药,在肛门直肠疾病中也有其不可取代的作用。肛门直肠疾病病机以湿、热、燥、火为主,治法分为保守疗法和手术疗法。保守疗法分为内治、外治,术后根据不同病变阶段可施以消、托、补等不同治法。从泻下攻积、清热泻火、凉血止血、清热解毒四个方面阐述大黄在肛门直肠疾病中的临床应用。     

早期下床活动在大隐静脉术后护理中的应用

文章对比观察早期下床活动应用于大隐静脉术后患者护理中的临床疗效、并发症发生情况。大隐静脉术后早期下床活动,可有效预防并发症发生,显著提高患者的护理满意度,适于临床推广使用。     

BDNF在甲状腺乳头状癌中的表达及临床意义

【目的】观察脑源性神经营养因子（brain derived neurotrophic factor ,BDNF）在甲状腺乳头状癌（Papillary thyroid carcinoma ,PTC）中的表达,探讨其与PTC发生及临床病理特征的关系。【方法】对56例P T C、22例甲状腺腺瘤、10例正常甲状腺组织,采用免疫组化链霉素抗生物素蛋白‐过氧化物酶法（S P法）检测BDNF的表达。【结果】BDNF在PTC、甲状腺腺瘤中的表达明显高于正常甲状腺组织（ P ＜0．05）;BDNF的表达水平与PTC肿块大小、有无淋巴结转移有关（ P ＜0．05）;BDNF表达水平与 PTC发病年龄、性别、临床分期无明显相关性（ P ＞0．05）;BDNF的表达水平与PTC的分化程度呈负相关（ r ＝－0．482,P＜0．05）。【结论】BDNF过表达可能与甲状腺腺瘤、PTC发生有关;BDNF表达水平为临床上判断PTC分化程度、淋巴结转移可能性提供有效参考,对指导PTC手术方式及辅助治疗具有实用价值。     

PET-CT在鼻咽癌诊断应用中的新进展

鼻咽癌早期症状不明显,发生部位较隐蔽,误诊率高。鼻咽癌恶性程度较高,易侵犯颅底组织及发生颈部淋巴结转移。最多见的鼻咽癌远处转移部位是骨（20%）,其次是肺部（13%）和肝脏（9%）。是否有淋巴结转移及远处转移是影响鼻咽癌患者预后的关键。因此,早期诊断、准确判断肿瘤侵犯范围和淋巴转移及远处转移状况显得尤为重要。传统的常规影像学检查CT及MRI主要显示人体组织解剖结构,对可疑病灶难以定性,且常遗漏小于5mm的病灶及难以发现远处转移灶,因此，难以全面评价鼻咽癌患者的状况。     

逆行荧光标记观察猫上橄榄复合体向内耳的投射

Objective To investigate the distribution and projective feature of cat olivocochlear neurons. Methods Eleven adult cats were divided into two groups randomly. The experimental group of eight cats was injected of I% cholera toxin B (CTB) to the left cochlea, while injected of 5% fluoro gold (FG) to the right cochlea. The control group of three cats was injected of saline to bilateral cochlea. After a survival time of 7 days, serial frozen sections were cut in the cat brainstem. All the sections were processed by immunofluoreseent procedure for CTB and FG, and the labeled olivocochlear neurons were observed byfluorescent microscope. Results In the experimental group, the mean total of olivocochlear neurons labeled by CTB and FG was 3210 ± 168, including lateral olivocochlear neurons (LOC, 2298 ± 120) and medial olivocochlear neurons (MOC,913 ± 64). The labeled neurons were divided into three different types according to their feature of projection: neurons which only projected to the ipsilateral cochlea, neurons which only projected to the contralateral cochlea, and double-labeled neurons which projected both to the ipsilateral and contralateral cochlea, but the double-labeled neurons comprised 3.9% and 15. 1% in the LOC and MOC system respectively. No labeled neurons were found in the control group. Conclusions There are three types of neurons in the cat olivocochlear system. The neurons which projected to the bilateral cochlea may distribute both in the LOC and MOC system.     

逆行荧光标记观察猫上橄榄复合体向内耳的投射

Objective To investigate the distribution and projective feature of cat olivocochlear neurons. Methods Eleven adult cats were divided into two groups randomly. The experimental group of eight cats was injected of I% cholera toxin B (CTB) to the left cochlea, while injected of 5% fluoro gold (FG) to the right cochlea. The control group of three cats was injected of saline to bilateral cochlea. After a survival time of 7 days, serial frozen sections were cut in the cat brainstem. All the sections were processed by immunofluoreseent procedure for CTB and FG, and the labeled olivocochlear neurons were observed byfluorescent microscope. Results In the experimental group, the mean total of olivocochlear neurons labeled by CTB and FG was 3210 ± 168, including lateral olivocochlear neurons (LOC, 2298 ± 120) and medial olivocochlear neurons (MOC,913 ± 64). The labeled neurons were divided into three different types according to their feature of projection: neurons which only projected to the ipsilateral cochlea, neurons which only projected to the contralateral cochlea, and double-labeled neurons which projected both to the ipsilateral and contralateral cochlea, but the double-labeled neurons comprised 3.9% and 15. 1% in the LOC and MOC system respectively. No labeled neurons were found in the control group. Conclusions There are three types of neurons in the cat olivocochlear system. The neurons which projected to the bilateral cochlea may distribute both in the LOC and MOC system.     

内镜清洗消毒的规范化管理

目的 规范内镜的清洗消毒管理工作,防止由内镜诊疗所造成的医院感染.方法 制定和完善内镜中心管理的各项制度,加大对内镜及有关消毒设施的投入,加强人员培训,规范清洗消毒工作流程.结果 清洗消毒后的内镜内壁和外壁的消毒合格率均达到100%.结论 内镜的清洗消毒的规范管理是内镜消毒灭菌达到标准的保证.     

中西医结合治疗高血压脑出血30例的体会

1998年7月～1999年1月,收治30例高血压脑出血患者,报告如下。1　病例与方法1.1　病例:30例患者中男17例,女13例;年龄43～87岁,平均(63.12±13.20)岁。颅脑CT证实出血部位:基底节区14例,丘脑7例,额叶4例,颞叶3例,顶叶1例,枕叶1例。既往均有高血压病史。1.2　治疗与护理:1迅速降颅压:20%甘露醇250ml静滴,每日3～4次,辅以速尿、地塞米松等。出血量大时及时手术治疗。2改善脑功能及催醒:醒脑静注射液20ml加生理盐水250ml静滴,每日2次。3防治并发症:昏迷者不能自主咳嗽排痰,要积极防治肺感染,适时行气管切开术,必要时据细菌培养和药敏试验选择有…