Effects of Yinchenhao Decoction(茵陈蒿汤) for Non-alcoholic Steatohepatitis in Rats and Study of the Mechanism

Objective:To observe the effects of Yinchenhao Decoction(茵陈蒿汤) for non-alcoholic steatohepatitis(NASH) in rats and study the mechanism.Methods:Total 18 male SD rats were randomly divided into a normal control group,a model group and a treatment group,6 rats in each group.Rats in the model and treatment groups were fed with high-fat forage for 10 weeks to prepare the NASH model,and the rats in the treatment group were administrated with Yinchenhao Decoction from the 6th week for 5 weeks.All rats were sacrificed at the end of the10th week and the samples were collected.Serum alanine aminotransferase(ALT) activity,tumor necrosis factor-α(TNF-α) level,and hepatic triglyceride(TG) and free fatty acid(FFA) contents were determined.Hepatic pathological changes were detected by HE staining.Results:Serum ALT activity,TNF-α level,hepatic TG and FFA contents,and the fatty deposition in hepatocytes were significantly reduced in the rats of the treatment group.Conclusion:Yinchenhao Decoction has good therapeutic effects for NASH,protecting the liver function and reducing the fatty deposition in liver,which are possibly related with reduction of FFA content and inhibition of TNF-α expression.     

均匀设计法筛选茵陈蒿汤抗脂肪肝脂质代谢异常的效应组分

目的:运用均匀设计法筛选茵陈蒿汤抗脂肪肝脂质代谢紊乱的效应组分及其最优剂量配比,探讨揭示复方配伍理论科学内涵的研究方法。方法:将茵陈蒿汤(茵陈、栀子、大黄)进行分组设计,分为6种中药组合,对高脂饮食诱导的大鼠脂肪肝模型进行干预,观察各组大鼠肝脏的病理变化,并以血清三酰甘油(TG)、肝组织TG、血清低密度脂蛋白(LDL)测定作为筛选指标,分析不同作用途径的效应组分及其最优剂量配比。结果:较之正常组,模型组血清TG、LDL和肝组织TG含量均明显升高(P<0.05~0.01),肝组织存在明显脂肪变性;各药物组上述脂肪病理改变程度均明显减轻。根据均匀设计实验逐步回归分析,降低血清TG和降低肝组织TG的效应组分分别是茵陈、栀子。而茵陈与大黄对降低血清LDL有明显疗效,以茵陈0.3g+大黄0.15g为最优剂量组合。结论:茵陈蒿汤通过多途径调节脂肪肝的脂质代谢,其不同作用途径存在特定的效应组分及相应的最优剂量配比。     

茵陈蒿汤调节高脂饮食诱导大鼠脂质代谢紊乱的作用机制

目的:探讨经方茵陈蒿汤调节高脂饮食诱导大鼠脂质代谢紊乱的作用机制。方法:将18只雄性SD大鼠随机分成正常组、模型组和茵陈蒿汤组,模型组和茵陈蒿汤组给予高脂饮食喂养10周,茵陈蒿汤组第6周起给予茵陈蒿汤灌胃5周。10周后处死大鼠,收集标本,检测血清甘油三酯(TG)、高密度脂蛋白胆固醇(HDL-C)、低密度脂蛋白胆固醇(LDL-C)、胆固醇(Ch)含量,肝组织TG含量,对肝脏组织进行HE染色。结果:茵陈蒿汤明显降低模型大鼠血清TG、LDL-C、Ch含量,增加血清HDL-C含量,显著降低肝脏TG含量,改善肝组织脂质沉积的病理状态。结论:茵陈蒿汤能够有效的调节高脂饮食诱导的大鼠血清和肝组织脂质代谢紊乱,其机制可能是促进HDL-C合成,减少Ch、TG、LDL-C在体内的蓄积。