Effects of Moxibustion at Shenque(CV 8)on Serum IL-12 Level and NK cell Activities in Mice with Transplanted Tumor

The study was to investigate the effect of moxibustion on the serum IL-2,IL-12 levels and NK cell andascitic tumor cell activities in H-22 mice with tumor and its mechanisms.The method used in the studywas that the IL-2 and IL-12 levels were determined by the double antibody method with ELISA,and theNK cell activities by the MTT measure.The results showed that moxibustion elevated the serum IL-2 andIL-12 levels and the NK cell activities of the mice with tumor,and the elevations were of significance ascompared with the controls(P<0.05).It is concluded that moxibustion can inhibit the growth of tumor,which is related to the increase of the serum IL-2 and IL-12 levels and the strengthening of NK cellactivities.     

Effects of Moxibustion at Shenque （CV 8） on Serum IL－12 Level and NK cell Activities in Mice with Transplanted Tumor

The study was to investigate the effect of moxibustion on the serum IL-2, IL-12 levels and NK cell and ascitic tumor cell activities in H-22 mice with tumor and its mechanisms. The method used in the study was that the IL-2 and IL-12 levels were determined by the double antibody method with ELISA, and the NK cell activities by the MTT measure. The results showed that moxibustion elevated the serum IL-2 and IL-12 levels and the NK cell activities of the mice with tumor, and the elevations were of significance as compared with the controls (P<0.05). It is concluded that moxibustion can inhibit the growth of tumor, which is related to the increase of the serum IL-2 and IL-12 levels and the strengthening of NK cell activities.     

艾灸神阙穴对移植瘤小鼠血清白细胞介素12及NK细胞活性的影响

目的：观察艾灸对H－22荷瘤小鼠血清白细胞介素2（IL－2）、白细胞介素12（IL－12）水平、NK细胞活性及腹水瘤细胞活性的影响，探讨艾灸抗肿瘤的机制。方法：采用双抗体夹心ELISA法，测定小鼠血清IL－12、IL－2水平；采用MTT法测定小鼠NK细胞活性。结果：艾灸能提高荷瘤小鼠血清IL－12、IL－2水平，与对照组比较差异有显著性9P＜0．05）；艾灸能提高荷瘤小鼠NK细胞杀伤率，与移植瘤对照组比较差异有显著性（P＜0．05）。结论：艾灸有抑制肿瘤生长的作用，此作用与其提高血清IL－2、IL－12水平，增强NK细胞杀伤活性有关。