基于BMI调节管电压的前瞻性大螺距CT冠状动脉成像研究

目的 CT冠状动脉成像目前已经广泛应用于临床,然而它的高辐射剂量一直受到人们的关注,降低CT冠状动脉检查的辐射剂量有重要的社会意义。文中探讨基于患者BMI调节管电压的前瞻性大螺距CT冠状动脉成像(CT coronary angiography,CTCA)的可行性。方法选取102例患者并行CTCA检查,其中实验组(72例)采用基于BMI调整管电压,BMI<23 kg/m2者选择70 k V,BMI 23~25 kg/m2之间者选择80 k V,BMI>25 kg/m2者行自动管电压选择技术;对照组(30例)采用120 k V前瞻性序列心电门控技术行CTCA。评估并比较2组的主观和客观图像质量及辐射剂量。结果实验组右冠状动脉CT值高于对照组[(562.2±152.3)HU vs(399.6±61.3)HU];实验组左冠状动脉主干CT值高于对照组[(571.1±159.5)HU vs(380.3±46.2)HU];实验组前降支CT值高于对照组[(559.4±140.9)HU vs(356.0±39.7)HU];实验组回旋支的CT值高于对照组[(553.0±168.6)HU vs(348.9±52.3)HU],差异均有统计学意义(P<0.001)。而实验组右冠状动脉、左冠状动脉主干、前降支和回旋支的对比噪音比与对照组比较,差异均无统计学意义(P>0.05)。2组主观图像质量的差异亦无统计学意义(P=0.604)。实验组的有效剂量明显低于对照组[(0.5±0.5)m Sv vs(4.5±1.3)m Sv,P<0.001]。实验组相比于对照组120 k V前瞻性序列扫描CTCA辐射有效剂量降低了88.9%。结论基于患者BMI调节管电压的前瞻性大螺距CTCA具有可行性。     

浅谈血液透析与血液灌流联合应用在治疗蜈蚣咬伤中的应用

<正>1资料与方法1.1一般资料2011年我科收治一名重症中毒男性患者,29岁,入院前12小时睡梦中突然疼醒,发现右腕部桡侧有2个小眼,中央有小疱,局部红肿,疼痛明显,自行用缝衣针挑刺,挤出清亮疱液,当时伴有寒战、发热、肢体乏力,未在意。之后上述症状逐渐加重,头痛、头晕、恶心明显来院就诊,详细询问病史,患者居住在仓库,仓库周围即是猪牛圈,常可见蜈蚣、壁虎等生物爬行,考虑为蜈蚣咬伤。     

上下直肌联结术治疗外直肌麻痹15例临床观察

目的 观察采用直肌联结手术治疗外直肌麻痹的手术效果(15例15眼).方法 回顾分析从2006年1月-2010年7月行直肌联结术联合同侧内直肌后徙,分步矫正外直肌麻痹的手术效果,效果较为满意.结论 二次手术后正位12例,有两例欠矫＜10°一例欠矫＞15°,外观上效果较理想术后,第一眼位正位,外转有一定改善.     

铅中毒对婴幼儿智能发育的影响

为了探讨铅中毒对婴幼儿智能发育的影响 ,作者于 2 0 0 2年 7～ 12月 ,对来湖北省妇幼保健院儿保科进行健康体检的 6个月～ 1岁的婴幼儿进行了血铅水平、智能发育和运动发育水平的测定 ,并对相关因素进行了分析 ,现报告如下。1　对象和方法1.1　对象　 2 0 0 2年 7～ 12月间到我院儿保科进行健康体检的散居儿童 ,剔除出生体重低下、早产、难产、新生儿窒息、重度黄疸 ,以及脑膜炎、脑外伤等影响智力的疾病 ,生长发育及外周血微量元素检测铁、锌均正常 ,年龄 6个月～ 1岁 ,其中男5 6岁 ,女 44例 ,共 10 0例。根据血铅水平将研究对象分为中毒…     

喂养方式与婴儿智能、运动发育的关系

[目的]分析不同喂养方式与婴儿智能、运动发育的关系,为促进婴幼儿健康成长提供依据.[方法]问卷调查后对资料进行卡方与方差分析.[结果]母乳喂养组婴儿在3、6、9、12个月龄时智能发育指数(mental devel-opment index,MDI),6、9和1 2个月龄时运动发育指数(psychomtive development index,PDI)均高于部分母乳喂养组与人工喂养组(P＜0.01或0.05).[结论]母乳喂养可促进婴儿智能和运动发育.     

转染血管内皮生长因子C反义寡核苷酸对子宫颈癌Hela细胞增生和凋亡的影响

Objective To explore effect on the proliferation and apoptosis of Hela cells in vitro by using vascular endothelial growth factor-C(VEGF-C) antisense oligonucleotides (ASODN). Methods VEGF-C ASODN was transfected into Hela cells by liposome-mediated; the cells transfected with the oligodeoxynuclecotide (SODN) and saline were used as control groups. The efficiency of transfection was detected by fluorescence microscope. The inhibitive rate of cell growth was detected with MTT methods. Apoptosis and cell cycle were evaluated using FCM. The mRNA expression of VEGF-C was determined by RT-PCR, and the expression of VEGF-C was determined by western blotting. Results VEGF-C ASODN had been transfected into Hela cells by liposome-mediated. The index of apoptosis after transfection 48 h was (19.39±1.81)%. G2/M phase cell increased after transfection, meanwhile the expression of VEGF-C degraded on the level of mRNA and protein (P<0.05). Conclusion Transfeeted with VEGF-C ASODN could down-regulate the expression of VEGF on the level of mRNA and protein, block the cell circle, inhibit cell proliferation and induce apoptosis in Hela cells in vitro.     

逆行导丝置入在中心静脉导管置换术中的临床研究

中心静脉导管置管术可避免给患者带来再次穿刺的痛苦，能延长静脉导管的留置时间，以确保化疗药物及其他刺激性药物如营养液的顺利完成。本研究将导丝逆行置入用于原位中心静脉导管置换术，提高了置管成功率，取得了满意效果，现报告如下。     

转染血管内皮生长因子C反义寡核苷酸对子宫颈癌Hela细胞增生和凋亡的影响

Objective To explore effect on the proliferation and apoptosis of Hela cells in vitro by using vascular endothelial growth factor-C(VEGF-C) antisense oligonucleotides (ASODN). Methods VEGF-C ASODN was transfected into Hela cells by liposome-mediated; the cells transfected with the oligodeoxynuclecotide (SODN) and saline were used as control groups. The efficiency of transfection was detected by fluorescence microscope. The inhibitive rate of cell growth was detected with MTT methods. Apoptosis and cell cycle were evaluated using FCM. The mRNA expression of VEGF-C was determined by RT-PCR, and the expression of VEGF-C was determined by western blotting. Results VEGF-C ASODN had been transfected into Hela cells by liposome-mediated. The index of apoptosis after transfection 48 h was (19.39±1.81)%. G2/M phase cell increased after transfection, meanwhile the expression of VEGF-C degraded on the level of mRNA and protein (P<0.05). Conclusion Transfeeted with VEGF-C ASODN could down-regulate the expression of VEGF on the level of mRNA and protein, block the cell circle, inhibit cell proliferation and induce apoptosis in Hela cells in vitro.     

转染血管内皮生长因子C反义寡核苷酸对子宫颈癌Hela细胞增生和凋亡的影响

Objective To explore effect on the proliferation and apoptosis of Hela cells in vitro by using vascular endothelial growth factor-C(VEGF-C) antisense oligonucleotides (ASODN). Methods VEGF-C ASODN was transfected into Hela cells by liposome-mediated; the cells transfected with the oligodeoxynuclecotide (SODN) and saline were used as control groups. The efficiency of transfection was detected by fluorescence microscope. The inhibitive rate of cell growth was detected with MTT methods. Apoptosis and cell cycle were evaluated using FCM. The mRNA expression of VEGF-C was determined by RT-PCR, and the expression of VEGF-C was determined by western blotting. Results VEGF-C ASODN had been transfected into Hela cells by liposome-mediated. The index of apoptosis after transfection 48 h was (19.39±1.81)%. G2/M phase cell increased after transfection, meanwhile the expression of VEGF-C degraded on the level of mRNA and protein (P<0.05). Conclusion Transfeeted with VEGF-C ASODN could down-regulate the expression of VEGF on the level of mRNA and protein, block the cell circle, inhibit cell proliferation and induce apoptosis in Hela cells in vitro.     

转染血管内皮生长因子C反义寡核苷酸对子宫颈癌Hela细胞增生和凋亡的影响

Objective To explore effect on the proliferation and apoptosis of Hela cells in vitro by using vascular endothelial growth factor-C(VEGF-C) antisense oligonucleotides (ASODN). Methods VEGF-C ASODN was transfected into Hela cells by liposome-mediated; the cells transfected with the oligodeoxynuclecotide (SODN) and saline were used as control groups. The efficiency of transfection was detected by fluorescence microscope. The inhibitive rate of cell growth was detected with MTT methods. Apoptosis and cell cycle were evaluated using FCM. The mRNA expression of VEGF-C was determined by RT-PCR, and the expression of VEGF-C was determined by western blotting. Results VEGF-C ASODN had been transfected into Hela cells by liposome-mediated. The index of apoptosis after transfection 48 h was (19.39±1.81)%. G2/M phase cell increased after transfection, meanwhile the expression of VEGF-C degraded on the level of mRNA and protein (P<0.05). Conclusion Transfeeted with VEGF-C ASODN could down-regulate the expression of VEGF on the level of mRNA and protein, block the cell circle, inhibit cell proliferation and induce apoptosis in Hela cells in vitro.