左金益胃汤治疗慢性萎缩性胃炎的疗效观察

目的 :观察左金益胃汤治疗慢性萎缩性胃炎的疗效。方法 :60例疣状胃炎患者随机分为两组。治疗组给予左金益胃汤(处方:太子参、麦冬、石斛、沙参、黄芩、黄连、吴茱萸、蒲公英、炙甘草、陈皮、茯苓)对照组给予泮托拉唑胶囊。观察治疗前后临床症状改善情况。结果 :治疗组显效率66.7%,总有效率93.3%,对照组显效率43.3%,总有效率76.6%,可见治疗组总有效率明显高于对照组,差异有统计学意义(P0.05)。结论 :左金益胃汤治疗慢性萎缩性胃炎的临床疗效显著。     

转入WIF-1或5-氮杂-2’-脱氧胞苷去甲基化抑制骨肉瘤模型鼠的肿瘤生长

BACKGROUND:WIF-1 is a tumor suppressor gene. Promoter hypermethylation causes WIF-1 down- regulation in most tumors. DNA methylation inhibitor can lead to gene demethylation and restore its expression. OBJECTIVE:To observe the differences of tumor pathology and, WIF-1 mRNA and protein changes using WIF-1 or 5-aza-2'-deoxycytidine demethylation in animal models of osteosarcoma. METHODS:Murine osteosarcoma models were established and divided into three groups. In the control group, no treatment was given. In the 5-aza-2'-deoxycytidine group, an appropriate amount of 5-aza-2'-deoxycytidine was injected in each mouse daily. In the WIF-1 group, an appropriate amount of Wnt/β-catenin signal transduction pathway inhibitor WIF-1 was injected in each mouse daily. Seven days after medication, the weight of nude mouse was weighed every 7 days. Short tumor diameter (a) and the long diameter (b) were measured. The relative tumor volume was calculated. The relative growth rate of tumor was calculated at 7, 14, 21, 28 and 56 days. Four nude mice from ach group were sacrificed by pulling the neck at 7, 14, 21, 28 and 56 days after medication. Tumor tissues were stripped and the weight of them was weighed. Pathological analysis of the tumor was conducted. The expression of WIF-1 protein and WIF-1 mRNA was detected in osteosarcoma at 56 days after medication in the three groups. RESULTS AND CONCLUSION: (1) Compared with the medication and control groups, the weight of nude mice was increased at 7, 14, 21, 28 and 56 days in the treatment group. No significant difference was found between the medication and control groups. (2) The tumor size was significantly smaller in the medication group than in the control group. WIF-1 mRNA and WIF-1 protein expression was increased in the medication group compared with the control group to different degrees. (3) Results suggested that WIF-1 gene promoter methylation is one of the mechanisms of the development of osteosarcoma. Use of WIF-1 or 5-aza-2'-deoxycytidine demethylation can inhibit tumor growth in animal models of osteosarcoma.     

《临证指南医案》从肝论治胃痛医案的证治分析

《临证指南医案》中有关胃痛的医案丰富,叶天士认为胃痛多与肝有关,提出"肝为起病之源,胃为传病之所"。分析胃痛医案可归纳出下列5种证型:肝气犯胃、肝郁化火犯胃、肝犯胃兼痰饮胸痹、郁伤脾胃阳虚、肝风犯胃液虚,从肝论治胃痛是其特色。探究叶桂从肝论治胃痛的病因病机、治法及用药对当今临床胃痛的诊治具有启迪意义。