ESTABLISHMENT OF A NOVEL CELL LINE (HNE_(1)) DERIVED FROM A NASOPHARYNGEAL CARCINOMA

A novel epithelial cell line, designated HNE1 was established from a biopsy specimen of a naso-pharyngeal carcinoma (NPC). Electron microscopic examination of the HNE1 cells demonstrated bi-directional differentiation, with some cells displaying features of poorly differentiated squamous cell carcinoma, while other cells appeared to have the morphology of poorly differentiated adenocarcinoma. The HNE1 cell line has been passaged more than 100 times over a period of one year. We recently reported that the Epstein-Barr virus (EBV) nuclear antigen (EBNA) was detected in a low pe rcentage of the HNE1 cells examined at subcultures 5-81; the cells were also shown to be EBV DNA positive. Tumorigenicity of the HNE1 cells was demonstrated by xentransplanta tion in athymic nude mice. The developed tumors were characterized as well-differentiated squamous cell carcinomas upon histological examination. Kar yotypic analysis of the HNE1 cells demonstrated an aneuploidy with a modal chromosomal number of 74 at passage     

长期接触化学致癌物的实验室工作人员的姊妹染色单体互换频率

本文观察了长期接触化学致癌物的实验室工作人员的姊妹染色单体互换(SCE)频率的变化;发现第一、第二次测定的结果极其接近,且其SCE值的明显高于正常的非接触者。结果还表明,接触者与非接触者外周淋巴细胞对交联剂/致突变剂丝裂霉素C有相同的敏感性,证实接触者的淋巴细胞染色体的稳定性没有改变。文中讨论了接触者SCE 值改变的可能机理,为长期接触致癌物的实验室工作人员改善工作环境,减轻致癌物接触水平提供了实验根据。     

剖腹产术后母乳喂养的心理护理

加强母乳喂养宣教工作,做好心理护理。协助哺乳、消除疼痛心理,纠正乳汁不足、消除产妇顾虑。     

论优质护理下持续负压引流在压疮伤口感染的应用体会

目的:探讨优质护理服务下封闭式负压引流在治疗压疮伤口的疗效观察和体会。方法:回顾收治院外带入和他科转入行压疮感染治疗的46例患者。随机分为对照组和治疗组各23例,对照组采用传统创面进行处理,治疗组采用持续封闭负压引流(VSD)技术处理,对两组治疗效果进行比较分析。结果:在46例烧伤感染性压疮患者中,治疗组患者的引流时间均为7~21 d,平均为10 d,形成健康新鲜的肉芽组织,肉芽生长的速度快,正常恢复的有效率为100%。对照组有效率为69.5%,治疗组患者的压疮创面愈合率显然高于对照组,平均愈合时间较对照组明显减少,差异有统计学意义(P<0.05)。结论:持续封闭负压引流治疗感染性压疮,疗效确切,可以有效的保持深部伤口的引流畅通,促进新鲜肉芽的生长,加速伤口愈合,积极配合优质的护理技术对压疮患者封闭负压引流的治疗起着不可忽视的作用。同时,做好患者的生活护理和基础护理尤为重要。     

二亚硝基哌嗪诱发人胚鼻咽上皮姊妹染色单体交换的观察

本实验用间接致癌物二亚硝基哌嗪(dinitrosopiperazine DNP)处理人胚鼻咽上皮细胞,以人胚肾上皮为对照,观察不同组织内DNP诱发的姊妹染色单体交换(Sistar Chromatid exchanges SCEs)的频率。结果表明:人胚鼻咽上皮可以活化DNP,表明诱发的SCE濒率增加,具量效关系;相同剂量DNP处理后,鼻咽上皮内诱发的SCEs频率明显高于肾脏上皮细胞,表现器官特异性的SCE反应,证实了人胚鼻咽上皮细胞有活化DNP酶系的性能。