液相芯片法验证蛋白质免疫印迹试验检测HIV-1结果的敏感性和特异性

目的初步分析采用液相芯片法研制人类免疫缺陷病毒(HIV)-1检测试剂的可行性。方法采用液相芯片技术用蛋白质免疫印迹试验(Western Blot)检测HIV-1阳性和阴性的标本;用HIV-1的gp120、gp41、p24抗原分别包被不同编号的免疫磁珠,与生物素化二抗、亲和素化荧光染料PE组成液相芯片检测系统,对标本进行检测分析。结果 55份标本经Western Blot法确认阳性样本检测符合率为100%;76份经酶联免疫吸附试验(ELISA)初检和复检阴性的标本检测HIV-1gp120、HIV-1gp41、HIV-1p24抗原均为阴性;86份经ELISA法检测初检和复检为阳性而Western Blot法确认为阴性的样本,其HIV-1gp120、HIV-1gp41、HIV-1p24单片段检测均为阴性。液相芯片法检测HIV-1抗体的敏感度和特异度均为100%。结论采用液相芯片技术组成的HIV-1抗体检测系统,其敏感度和特异度均优于ELISA法检测。     

Study on graded therapy of hemophilic arthritis by integrative traditional Chinese and Western medicine

Objective:To study the effect and safety of graded therapy featuring integrative traditional Chinese and Western medicine for the treatment of hemophilic arthritis.Methods:Forty patients with hemophilic arthritis were hospitalized randomly,with their blood coagulation factor activity determined by one-stage method and their arthritis classified into 4 stages.The treatment was applied according to the stage of arthritis and finding of intra-articular cavity puncture.For stageⅠ,based on the principle of RICE(rest,ice,compression and elevation),1.8g of Xuefuda(血府达)was medicated orally once per day,intravenous dripping of 250mL of hemostasis mixture twice a day and 1.2g of clindamycin per day were also given for hemostasis and anti-inflammation.For stageⅡ-Ⅲ,Kangyanling(抗炎灵)was additionally administered via intra-articular cavity injection twice a week,2mL every time,for 5-6 times in total.For stageⅣ,the drug for intra-articular cavity injection was replaced with 25mg of sodium hyaluronate and the frequency of injection reduced to every two weeks,for 5-6 times in total.Coagulation factorsⅢandⅣas well as blood plasma were not given in the whole treatment course.Short-term therapeutic effects and adverse reaction in patients were evaluated,and the long-term effects were followed-up after patients left the hospital with 6-month consolidation therapy by Xuefuda.Results:After a 3-week treatment,33 patients (82.5%)were completely remitted;5(12.5%)were partially remitted and 2(5.0%)un-remitted,setting the short-term effective rate at 95.0%(38 cases).The 6-month follow-up showed that except for a relapse in 2 and 4 patients of stageⅢandⅣrespectively,long-term remission displayed in all the other 34 patients,with the remission sustaining rate being 85.0%.No complication such as an infection,bleeding or aggravating pain occurred in the 215 times intra-articular puncturing conducted in the 40 patients.Normal figures were shown in liver and kidney function,electrolytes,ECG,blood glucose and routine test of blood and urine throughout the course.Conclusion:The graded treatment of integrative medicine for hemophilia with non-blood preparation has a favorable effect and is safe or without any adverse reaction,which opens a high efficacy and new safe path and thinking for the treatment of and deformity prevention in the hemophilic patients.     

九型人格在护理硕士研究生人才培养中的应用研究

目的通过探讨九型人格在护理硕士研究生人才培养中的应用,以期为护理硕士研究生人才培养提供一些方法。方法利用学校图书馆数据库进行文献检索,根据相关性保留部分文献后再对九型人格相关理论和各型人格特征进行分析和归纳总结。结果将九型人格应用于护理硕士研究生人才培养中,结合传统的培养方法和手段,可以有效地提高其教学、实践、管理以及科研和创新能力。结论将九型人格应用于护理硕士研究生人才培养中,可以对其教学、实践、管理、科研以及学术水平的提高提供帮助。     

超声波提取防风色原酮的研究

目的 优选防风色原酮的超声波提取工艺.方法 以升麻素苷、升麻素、5-O-甲基维斯阿米醇苷和亥茅酚苷的含量为指标,采用正交实验法优选超声波提取防风色原酮的最佳工艺.结果 最佳提取条件为料液比为1:30,超声时间45 min,超声温度50℃,超声频率90 kHz.结论 采用超声波提取技术提取防风色原酮,提取时间短,提取效率高,可用于防风色原酮的提取.     

Study on the efficacy and safety of Xueyou Mixture (血友合剂) in treating hemophilia

Objective:To observe the effect of Xueyou Mixture(血友合剂,XYM) on blood coagulation factors and its safety in treating hemophilia.Methods:To the randomly selected 65 inpatients of hemophilia,XYM was administered accompanied with intravenous dripping of liver cell growth factor 60-100 mg once a day to protect the liver,with no blood products like concentrated Ⅷ and FⅨ factors or blood plasma given.The treatment lasted for 3 weeks.The short-term eff icacy and adverse reactions were observed.The long-term efficacy in patients was observed in a follow-up study of 6-12 months after they were discharged from the hospital but continuously took XYM orally.Results:The short-term markedly effective rate in the patients was 95.38%(62/65).After they were treated for 3 weeks,the level of FⅧ factor activity increased in 56 patients of type A from(3.32±2.21) % to(4.18±2.23) %,and in 9 of type B from(4.92±1.81) % to(5.64±1.96) %.Compared with that before treatment,the difference was signif icant in both of them(P<0.01).No obvious adverse reaction was found in the treatment period.The follow-up study showed that in 22 patients of type A,the FⅧ factor activity ratio increased from(3.25±2.11) % to(6.31±2.16) %,(8.36±1.05) %,and(16.38±2.71) % in the 2nd,3rd and 6th month after discharge respectively,all showing significant difference to that before treatment(P<0.01);and in 4 patients of type B,it increased from(4.15±2.26) % to 7.8% and 11.6%(mean value) in the 2nd and 6th month respectively.Conclusion:XYM could raise the activity of factors Ⅷ and Ⅸ in patients with hemophilia,and the degree of the rise is related with the duration of the therapy,with no obvious adverse reaction,which strikes out a new path and new train of thinking for the treatment of the disease by non-blood preparation.     

中医药治疗多囊卵巢综合征思路与方法的初探

多囊卵巢综合征是一种多起因、病理生理和临床表现多态性的综合征,是青少年到育龄妇女发生高雄激素性无排卵现象中最常见的病种,属妇科疑难杂症。现通过对多囊卵巢综合征病因病机、治疗经验、个案举例的思考与总结,指出中医中药对治疗多囊卵巢综合征存在明显的治疗优势。     

鲍曼不动杆菌73株特征及耐药性分析

目的 分析鲍曼不动杆菌临床分布及其耐药性情况,为临床有效治疗感染性疾病提供依据.方法 对73株鲍曼不动杆菌感染患者的临床资料进行回顾性分析,并统计分析普通培养加血培养后进行鉴定和药敏的实验结果.结果 73株鲍曼不动杆菌感染患者平均年龄(55.69±20.72)岁,其中≥60岁为34例(占46.6％).临床标本以痰液为主,占65.7％(48株),其次为多种组织分泌物9例(12.3％)和尿液(5例,6.9％).呼吸内科13例为最多(占17.8％),其次为ICU病房和外科,分别为10例和8例.73株鲍曼不动杆菌对临床常用抗菌药物均存在一定的耐药性,其中对头孢噻肟、阿米卡星等10种临床抗菌药物的菌株耐药率均大于50％,尤以庆大霉素(65.7％)为最高.8株细菌(11.0％)对亚胺培南耐药,其中6株细菌对11种抗菌药物广泛耐药.结论 鲍曼不动杆菌具有日益严重的临床抗菌药物耐药特征,应该合理有效使用抗菌药物,加强高危患者和病区管理以减少院内感染的发生.